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Effect of GeO2 Concentration on Hydroponically-Grown Lettuce (Lactuca sativa)
이긍표,박희승,Jae Hee Won,In-Myung Choi,Kuen Woo Park 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2
Various concentrations of germanium dioxide (GeO2) was treated such as 1.0, 2.0, 4.0, 6.0, and 8.0 mgL-1 to lettuce (Lactuca sativa cv. Cheongchima) cultured in hydoponics for 4 weeks. Ge was linearly accumulated in the plants, primarily in the roots, as GeO2 concentration in the nutrient solution increased from 1.0 to 8.0 mgL-1. In addition, Ge once taken by the root was hardly moved to the shoot. The plant showed the best growth at 4.0 mgL-1 GeO2 for 7 days. In all ranges of the Ge treatment, the plant showed no phenotypic changes in leaf shape and color including necrosis or chlorosis, but the roots were stunted and thickened. The roots treated with GeO2 at 6 or 8 mgL-1 showed thickened cortex, but they had similar sizes of cells and vascular tissues. The leaf tissues treated with GeO2 had denser mesophyll cells than the control. Interestingly, the Ge-treated leaf looked harder, more greenish, and more glossy than the control. With increasing GeO2 concentration in the nutrient solution, the root activity was decreased, whereas lipid peroxidation increased.
Genetic Mapping of Resistant Genes in Brassica pekinensis Against Plasmodiophora brassicae Race 6
이긍표,백남권,박권우 한국식물병리학회 2002 Plant Pathology Journal Vol.18 No.5
Inbred lines of Chinese cabbage KU-101 (resistant line against Plasmodiophora brassicae race 6) and CS-113 (susceptible line) were crossed and their progeny lines F1, BC1F1, F2, and F3 were produced for the construction of the genetic linkage map of P. brassicae race 6- resistant Brassica campestris ssp. pekinensis genome. Restriction fragment length polymorphism (RFLP) was applied to compare between parents and their F2 progenies with a total of 192 probes and 5 restriction enzymes. The constructed RFLP map covered 1,104 cM with a mean distance between genetic marker of 8.0 cM, and produced 10 linkage groups having 121 genetic loci. The loci of P. brassicae race 6 (CR6)-resistant Brassica genome were determined by interval mapping of quantitative trait loci (QTL), which resulted from bioassay using the same race of the fungi in F3 population. Resistant loci were estimated in numbers 1 (G1) and 3 (G3) linkage groups. In the regression test, G1 had a value of 4.8 logarithm of odd (LOD) score, while G3 had values of 4.2-7.2. Given these results, the location of the CR6-resistant loci within the Brassica genome map can now be addressed.