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이중 활성 곤충병원성 곰팡이 4균주에 대한 최적 배양 배지 선발 및 다양한 항균활성 평가
윤휘건 ( Hwi-geon Yun ),곽원석 ( Won-seok Gwak ),우수동 ( Soo-dong Woo ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.3
Selection of the optimal culture medium and evaluation of the antimicrobial activity against various phytopathogens were performed for four entomopathogenic fungal isolates with excellent insecticidal and antimicrobial activity against the two-spotted spider mite (Tetranychus urticae), green peach aphid (Myzus persicae), and gray mold (Botrytis cinerea). The optimal medium was selected by measuring the amount of blastospore production and the antifungal activity of the culture medium. On the basis of these experiments, GY medium was selected for Beauveria bassiana 2R-3-3-1 and Metarhizium anisopliae 4-2, SD3, and PDB medium for B. bassiana SD15. The antimicrobial activity test against other phytopathogens indicated that all four isolates showed high antifungal activities against Colletotrichum acutatum and Sclerotinia sclerotiorum. However, for Phytophthora capsici and C. fructicola, only M. anisopliae SD3 showed a high antifungal activity against P. capsici, and the other three isolates had little activity. Antibacterial activity against Clavibacter michiganensis subsp. michiganensis was high in two isolates of M. anisopliae but not in two isolates of B. bassiana . Thus, it was confirmed that entomopathogenic fungi effective for pest control could be effectively used as a control agent for various plant diseases.
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제조체 저항성 형질전환 벼(CPPO06)에서의 곤충상 조사
배성민, 유미라, 윤휘건, 신태영, 최재방, 이원우, 김희정, 김흥태, 우수동 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.3
Occurrence of insects on the transgenic rice plant which is resistant to the herbicide was assessed and compared with other varieties of rice in the fields located at Cheongwon, Suwon and Gwangju in Korea. Transgenic rice (CPPO06) has a protox gene and is resistant to the protox-inhibitor herbicide. The occurrence and population of several major pests such as brown plant hopper, small brown planthopper, whitebacked planthopper, rice green leafhopper, rice stemborer and rice leaf roller, and other insects were investigated in 2011 and 2012. The rice green leafhopper among major pests was observed only in the field of Suwon and Gwangju in 2012. There was no significant difference in the occurrence of rice green leafhopper between CPPO06 and other rice varieties. Various insects except major pests were also observed in all fields but there was no significant difference between rice varieties. To assess eco-safety of transgenic rice CPPO06 using insects, more continuous and laboratory experiments should be performed.
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