인삼의 세포독성분획의 작용기작에 관한 연구(I) -인삼중의 Petroleum Ether 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향-

윤연숙,이세영,김병수,윤택구,Yun, Yeon-Sook,Lee, Se-Yong,Kim, Byung-Soo,Yun, Taik-Koo 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.4

고려인삼의 Petroleum ether추출물이 실험관내 배양중인 쥐의 백혈병성임파종양, L5178Y 세포와 쥐의 복수육종암 Sarcoma 180세포에 대하여 세포독성이 있음을 확인하고 이와 같은 세포독성과 고분자물질합성 억제작용과의 관련성을 검토하였다. 고려 인삼으로부터 추출된 Petroleum ether추출물은 실험관내 배양중인 L5178Y세포와 Sarcoma 180세포의 증식을 억제하였으며 실험관내의 Sarcoma 180세포에서 DNA, RNA 및 단백질 합성도 억제하였는데 이 분획의 세포독성과 고분자물질 합성 억제 작용은 모두 거의 지수함수적으로 농도에 비례하는 양상으로 나타났다. 따라서 이 분획의 세포독성은 고분자물질 합성 억제작용과 관련성이 있는 것으로 생각되며 DNA와 RNA 합성보다는 단백질 합성을 더 예민하게 억제하였으므로 특히 단백질 합성 억제 작용과 밀접한 관계가 있는 것으로 생각된다. 이 분획에 의한 단백질 합성 억제효과는 폴리좀 형성을 방해함으로서 기인하였는데 90%이상의 단백질 합성을 억제하는 농도에서 폴리좀이 모노좀으로 분해되는 것을 촉진하였으며 모노좀으부터 폴리좀 형성하는 것을 억제하였다. 그러나 메센져 RNA의 분해는 일으키지 않았다. 그러므로 인삼 중의 Petroleum ether분획 이 단백질 합성과정중에서 펩티드 합성개시 과정을 억제한다고 할 수 있다. The petroleum ether fraction from Korean Ginseng roots has been proved to be cytotoxic to murine lymphocytic leukemia L5178Y cells and murine Sarcoma 180 cells. This petroleum ether fraction which was extracted from Korean ginseng roots inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro. And it also inhibited DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells in vitro. The cytotoxicity of this fraction and it's inhibitory effect on biosynthesis of macromolecules were exponential function of its concentration. The cytotoxicity correlated to the inhibitory effect on the biosynthesis of macromolecules, especially to the inhibitory action on protein synthesis. The inhibitory action of this fraction on the protein synthesis was due to the inhibition of polysome formation: at the concentration which causes 90% inhibition of protein synthesis all the polysomes were dissociated into monosomes. Since messenger RNA degradation was not occurred at this concentration, petroleum ether fraction seems to interfere specifically the initiation of polypeptide synthesis.

인삼의 세포독성분획의 작용기작에 관한 연구 ( 1 ) - 인삼중의 Petroleum Ether 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향

윤연숙,이세영,김병수,윤택구 ( Yeon Sook Yun,Se Yong Lee,Byung Soo Kim,Taik Koo Yun 생화학분자생물학회 1980 BMB Reports Vol.13 No.4

The petroleum ether fraction from Korean Ginseng roots has been proved to be cytotoxic to murine lymphocytic leukemia L5178Y cells and murine Sarcoma 180 cells. This petroleum ether fraction which was extracted from Korean ginseng roots inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro, And it also inhibited DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells in vitro. The cytotoxicity of this fraction and it`s inhibitory effect on biosynthesis of macromolecules were exponential function of its concentration, The cytotoxicity correlated to the inhibitory effect on the biosynthesis of macromolecules, especially to the inhibitory action on protein synthesis, The inhibitory action of this fraction on the protein synthesis was due to the inhibition of polysome formation: at the concentration which causes 90% inhibition of protein synthesis all the polysomes were dissociated into monosomes, Since messenger RNA degradation was not occurred at this concentration, petroleum ether fraction seems to interfere specifically the initiation of polypeptide synthesis.

인삼의 세포독성분획의 작용기작에 관한 연구 ( 2 ) - 인삼중의 Ethyl acetate 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향

윤연숙,이세영,기병수,윤택구 ( Yeon Sook Yun,Se Yong Lee,Byung Soo Kim,Taik Koo Yun ) 생화학분자생물학회 1980 BMB Reports Vol.13 No.4

The ethyl acetate fraction from Korean Ginseng roots has been shown to be cytotoxic to murine lymphocyitic leukemia L5178Y cells and murine Sarcoma 180 cells. This ethyl acetate fraction which was extracted from Korean Ginseng root inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro by an exponential function of its concentration, DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells were also inhibited in vitro in a similar fashion, The cytotoxicity of this fraction correlates to the inhibitory effect on the biosynthesis of marcomolecules. The synthesis of DNA and RNA were much more sensitive to this fraction than that of potein, indicating that the inhibitory action on the biosynthesis of nucleic acids is responsible for the inhibition of protein synthesis, However, the cytotoxic activity of this fraction does not seem to be due to the inhibitory activity on the DNA synthesis or to the gross inhibition of RNA synthesis since the synthesis of DNA and RNA were inhibited only 40-45% at the concentration where 70% cell death occurred, In order to examine the possibility that the cytotoxicity of the ethyl acetate fraction is due to the inhibitory activity on the particular RNA species, we compared the inhibitory activity of this fraction on various RNA species with that of actinomycin D. At lower concentrations, this fraction inhibited the synthesis of the heterogeneous nuclear RNA much more than any other RNA species. The synthesis of ribosomal RNA was also sensitive, while the messenger RNA and transfer RNA synthesis were relatively insensitive to this fraction. At high concentration, the fraction inhibited the synthesis of all species of RNA.

Effect of Red Ginseng on Natural Killer Cell Activity in Mice with Lung Adenoma Induced by Urethan and Benzo(a) pyrene

윤연숙,조성기,문혜선,김영주,오영란,윤택구,Yun, Yeon-Sook,Jo, Sung-Kee,Moon, Hae-Sun,Kim, Young-Ju,Oh, Yeong-Ran,Yun, Taik-Koo 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1

홍삼의 항발암작용 기전을 규명하기 위한 목적의 일환으로 홍삼추출물이 urethan 및 benzo(a)pyrene을 투여한 마우스에서 natural killer(N.K) 세포 활성도 및 폐선종 발생에 미치는 영향을 발암물질 처리 후 48주 동안 검색하였다. N.K 세포 활성도는 urethan 및 benzo(a)pyrene의 처리에 의하여 현저히 저하되었다. 이와 같은 N.K 세포 활성도의 저하는 상기 발암물질 투여 후 4주부터 나타나 24주까지 계속되었으며 홍삼투여에 의하여 정 상대조군의 수준으로 되돌아 왔다. 동시에 urethan에 의해 6주시부터 유발되기 시작한 페선종 역시 홍삼투여에 의해 그 발생빈도가 현저히 억제되었다. 반면 benzo(a)pyrene에 의해 유발된 폐선종은 48주시에 나타나기 시작하였는데 이 시기는 N.K 세포 활성도가 대조군에서 조차 너무 낮은 수준으로 떨어져 있어 홍삼의 영향을 받을 수 없었는바 홍삼 투여에 의한 폐선종발생빈도 억제효과는 나타나지 않았다. 결론적으로 저자들은 본 연구를 통하여 홍삼에 의한 항발암 효과는 N.K 세포 활성도의 증대와 관련되어 있음을 알 수 있었다. It was previously reported that red ginseng extract inhibited carcinogenesis by urethan, DMBA and aflatoxin $B_1$, (Cancer Detection and Prevention, 6: 515-525, 1983). In an attempt to investigate the mechanism of the anticarcinogenic effect of ginseng, the N.K activity and the incidence of lung adenoma were followed over a period of 48 weeks post injection with urethan or benzo(a)pyrene. The N.K activity was markedly depressed from 4 weeks to 24 weeks after injection of carcinogens. This decreased N.K activity was returned to the level of controls by administration of ginseng. At the same time, lower incidence of lung adenoma noted following administration of ginseng to urethan-injected mice. However, the lung adenoma induced by benzo(a)pyrene began to occure at 48 weeks in which N.K activity had naturally declined to a level too low to be affected by ginseng, and administration of ginseng did not decrease the incidence. In conclusion, these results suggest that the anticarcinogenic effect of ginseng may be related to the augmentation of N.K activity.

홍삼이 Urethan 및 Benzo ( a ) pyrene 에 의하여 폐선종이 유발된 마우스에서 Natural killer 세포 활성도에 미치는 영향

윤연숙,조성기,문혜선,김영주,오영란,윤택구 ( Yeon Sook Yun,Sung Kee Jo,Hae Sun Moon,Young Ju Kim,Yeong Ran Oh,Taik Koo Yun ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1

It was previously reported that red ginseng extract inhibited carcinogenesis by urethan, DMBA and aflatoxin B₁, (Cancer Detection and Prevention, 6: 515-525, 1983). In an attempt to investigate the mechanism of the anticarcinogenic effect of ginseng, the N.K activity and the incidence of lung adenoma were followed over a period of 48 weeks post injection with urethan or benzo(a)pyrene. The N.K activity was markedly depressed from 4 weeks to 24 weeks after injection of carcinogens. This decreased N.K activity was returned to the level of controls by administration of ginseng. At the same time, lower incidence of lung adenoma noted following administration of ginseng to urethan-injected mice. However, the lung adenoma induced by benzo(a)pyrene began to occure at 48 weeks in which N.K activity had naturally declined to a level too low to be affected by ginseng, and administration of ginseng did not decrease the incidence. In conclusion, these results suggest that the anticarcinogenic effect of ginseng may be related to the augmentation of N.K activity.