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유정겸(J.K.Yu),신동훈(D.H.Shin),오광석(K.S.Oh),윤지원(J.W.Yoon),이경수(K.S.Yi) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
This paper presents a simplified tractor model which is developed based on a resonance analysis of a real tractor. Since two most interesting behaviors in relation to resonance are vertical vibration and pitch motion, this paper focuses on the analysis of resonance characteristics with respect to these two behaviors. In order to analyze the two resonance characteristics easily, half tractor model and quarter tractor model are introduced. These models consist of three parts: seat, cabin, tractor body. The results from the analysis are validated by comparing simulation results with experiment data.
韓國人의 ABO 및 Rh-Incompatibility에 關한 統計學的 硏究
尹址洹,金吉雄,曺澈,高光三,高宇永,金成源 中央醫學社 1973 中央醫學 Vol.24 No.6
In Korean population, ABO-incompatible matings constitute 44.15 percentage of all matings. Comparing to ABO-incompatible mating rate of American population, it is 10 percentage higher than the United States. for B group in Korea is 16 percentage higher than in American population. Calculating these by geriotypic mating form, it is about 44% (43.998015%), which includes genotypic compatible mating groups except mating group AB (8) X 0 (_?) (I n H B X (° 1'). The total percentage of these genotypic compatible matings in phenotypic incompatible mating groups is about 16 percentage. If we reduce these percentage from phenotypic incompatible mating rate 44%, the actual. genotypic mating rate will be estimated as 27.5% in Korean population. Mean number of children from ABO-incompatible mating groups shows 4.8 (person), while that of children from ABO-compatible mating groups indicates 5.43 (person). The deviation between two means is 0.63(person). By t-test, t=2,470, and P<0.05, so the difference between two means is significant. Evidence from the investigations of two thousands cards by random sampling method through Korea indicates that such incompatibility contributes to early period was 13.3 days. Duration from onset to hospitalization was 7.1 8. Most of all patients (64-65'10 were isolated in the Kim-Chun Provincial.vspontaneous abortion and miscarriage but by no means the only cause. Embryonic and early fetal death occur in compatible matings, also and conversely, they occur in only a small minority of pregnancies in incompatible matings. Percentage of Rh-incompatible matings is 0.5%. This small percentage is only because of a very few people in Korean population. Mean number of children from these matings is 1 (person), while the mean number of children from Rhcompatible matings (99. 5%) shows 5. 11 (person). Difference between these two means is highly significant. The cases of Rh-incompatibility and ABO-incompatibility combined are found 0. 1 percentage of all matings, and percentage of these matings from heterozygote is only 0. 05%. ABO-incompatibility lies in the interesting relationship between ABO-incompatibility and Rh-incompatibility. Statistical evidence indicates that ABO-incompatibility may prevent the development of all harmful effects of Rh-incompatibility. In Korean population, the percentage of Blood Group is; group A; 33%, group B; 26%, group 0; 32%, and group AB; 9%. Gene frequencies of this population P;. 24, q; .19, and r; .57. The percentage of Rh+ people is 99.6% and that of Rh- people is 0.4%. These data are obtained from investigation of 4,000 people by random sampling method.
Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)
양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1
To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.