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서해안 해수로부터 분리한 한천분해 해양미생물 Pseudoalteromonas sp. H9의 동정 및 특성 연구
지원재,윤영상,김종희,홍순광,Chi, Won-Jae,Youn, Young Sang,Kim, Jong-Hee,Hong, Soon-Kwang 한국미생물·생명공학회 2015 한국미생물·생명공학회지 Vol.43 No.2
대한민국 대천 해수로부터 agarase를 생산하는 균주 H9을 분리하였다. 본 균주는 16S rRNA 염기 염기서열 분석결과로부터 Pseudoalteromonas espejiana NCIMB2127<sup>T</sup> (98.98%), Pseudoalteromonas carrageenovora ATCC12662<sup>T</sup> (98.78%), Pseudoalteromonas atlantica IAM12927<sup>T</sup> (98.64%), Pseudoalteromonas issachenkonii KMM3549<sup>T</sup> (98.63%) 등과 높은 상동성을 보였다. 균주 H9은 genomic DNA 내 G+C 농도가 41.56%이고 주요 퀴논으로 quinone-8을 포함하고 있다. 균주 H9의 주요 지방산으로 C<sub>16:1</sub>ω7c (34.3%), C<sub>16:0</sub> (23.72%), C<sub>18:1</sub>ω7c (13.64%) 등이 포함되었다. 이러한 유전적, 생리적 특성에 따라 균주 H9은 Pseudoalteromonas 속의 균으로 분류하여 Pseudoalteromonas sp. H9으로 명명하였다. 균주 H9이 세포외부로 분비하는 총 agarase는 40-45℃와 pH 7.0-8.0의 조건에서 높은 효소 활성을 갖으며, agarose를 분해하여 (neo)agarotetraose와 (neo)agarohexaose를 생산하였다. 균주 H9은 한천분해를 위해 유용하게 사용될 수 있으며, 다양한 생리활성을 갖는 (neo)agarooligosaccharide는 기능성 식품, 화장품 등의 산업에 유용하게 사용될 수 있을 것으로 기대된다. An agarolytic marine bacterium (H9) was isolated from the coastal seawater of the West Sea, South Korea. The isolate, H9, was gram-negative and rod-shaped with a smooth surface and polar flagellum. Cells grew at 20-30℃, between pH 5.0 and 9.0, and in ASW-YP (Artificial Sea Water-Yeast extract, Peptone) media containing 1-5% (w/v) NaCl. The G+C content was 41.56 mol%. The predominant isoprenoid quinone in strain H9 was ubiquinone-8. The major fatty acids (>10%) were C<sub>16:1</sub>ω7c (34.3%), C<sub>16:0</sub> (23.72%), and C<sub>18:1</sub>ω7c (13.64%). Based on 16S rRNA gene sequencing, and biochemical and chemotaxonomic characterization, the strain was designated as Pseudoalteromonas sp. H9 (=KCTC23887). In liquid culture supplemented with 0.2% agar, the cell density and agarase activity reached a maximum level of OD = 4.32 (48 h) and OD = 3.87 (24 h), respectively. The optimum pH and temperature for the extracellular crude agarases of H9 were 7.0 and 40℃, respectively. Thin-layer chromatography analysis of the agarase hydrolysis products revealed that the crude agarases hydrolyze agarose into neoagarotetraose and neoagarohexaose. Therefore, the new agar-degrading strain, H9, can be applicable for the production of valuable neoagarooligosaccharides and for the complete degradation of agar in bio-industries.
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김주용,위다연,윤영상,Kim, Joo-Yong,Wi, Da-Youn,Yoon, Young-Sang 한국섬유공학회 2007 한국섬유공학회지 Vol.44 No.1
The electrostatic property of textile fabrics has been one of important factors determining a quality of fabrics. However, it has not been easy to evaluate since it was too sensitive to the environmental variables and the types of test methods. In this study, we designed simultaneous measurements system of tribe-electrification and charge decay with an estimate method of electrostatic property for textile fabrics. An automatic tension reliever of rubbing cloth and data acquisition system were attached to the rotary rubbing system for further detailed analysis. Through the system developed, fabric's charging and discharging phenomena and characteristics of charge decay time by the rotary rubbing were elucidated in details. It was found that wool fabrics rapidly charged with electricity at the initial stage, and immediately followed by the steady state charge.
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