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방사선 조사가 파보바이러스 예방용 IgY를 함유하는 난황분말의 살균과 안정성에 미치는 영향
차인석(In-Seok Cha),박민근(Min-keun Park),이기정(Kie-Jeung Lee),윤세희(Sae-Hee Yoon),김정옥(Jeung-Ok Kim),김승희(Seung-Hee Kim),양재혁(Jae-Hyuk Yang),정종태(Jong-Tae Cheong),임윤규(Yoon-Kyu Lim),황규계(Kyu-Kye Hwang) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.3
The gamma irradiation has been used as a method of sterilization which is often needed for long-term storage of various dried-foods by preventing them from decomposition. In present study, we tested the sterilization effects of gamma irradiation on the egg yolk powder containing anti-canine parvovirus yolk antibody (IgY) which can be used for controling canine parvovirus infection. Egg yolk powder was gamma irradiated at various levels (0.08, 0.4, 2, 10 and 50 kGy), stored at 4℃ and 20℃ for 60 days, and measured on sterility and antibody titer. Counting of aerobic bacteria indicated that samples irradiated at 0.4, 2 and 10 kGy were 89-94, 93-99 and 100% sterilized, respectively compared to non- irradiated control. ELISA test showed that gamma irradiation at 2 kGy or less did not make significant influence on antibody titer, whereas irradiation at 10 kGy induced about 15% reduction of antibody titer. Neither bacterial count nor antibody titer was affected by storage temperature after gamma irradiation. Conclusively, the proper level of gamma irradiation was about 2 kGy to achieve maximum sterilization on egg yolk powder without diminishing antibody titer.
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윤세희,서영석 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1
Recently, it has been known that neonatal capsaicin treatment severely reduces the number of type B spinal ganglia neurons selectively, and also decreases many of the unmyelinated and some of the fine myelinated axons in dorsal roots and peripheral nerves. Using these effects of capsaicin, present study was attempted to clarify the relationships between the sizes of S₁ spinal ganglion cells and the diameters of their axons in dorsal roots and peripheral nerves of the rats. The normal and capsaicin-pretreated adult rats were operated microscopically to remove the somatic and visceral motor axons from their peripheral nerves, and one week later, fixed by transcardiac perfusion techique. The ganglia were embedded in paraffin for light microscopy, and the dorsal roots and peripheral nerves in plastic mixture for electron microscopy. All the ganglion cells and axons were counted, and the diameters of ganglion cells and myelinated axons were measured by computed planimeter. The results obtained were summarized as followings. 1. In dorsal root ganglia, the diameters of neuronal bodies were in the range of 5~45㎛, and averaged 18.22㎛ in a normal group and 20.54㎛ in an experimental group. Neonatal capsaicin-treatment decreased 44.5% of total number of neurons which were small ones less than 17.5% in diameter. 2. In the dorsal roots, the unmyelinated axons decreased 52.496 and myelinated axons, 11.2% in an experimental group. The diameters of myelinated axons were in the range of 0.3~11.0㎛, and averaged 2.72㎛ in a normal group and 3.14㎛ in an experimental group. The perished axons by neonatal capsaicin-treatment were appeared to be less than 2㎛ in diameter. 3. In the peripheral nerves, the unmyelinated axons decreased 55.1% and the myelinated axons, 13.7% in an experimental group. The diameters of myelinated axons were in the range of 0.5~11.5㎛, and averaged 3.07㎛ in a normal group and 3.26㎛ in an experimental group. The perished axons were less than 3㎛ in diameter. 4. Therefore, it is borne out that the small ganglia cells less than 17.5㎛ in diameter give rise to the unmyelinated axons to the dorsal roots and peripheral nerves, and myelinated axons less than 2pm in diameter to the dorsal root and those less than 3㎛ in diameter to the peripheral nerve.
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