Estrogen의 화학적 측정법에 대한 검토

유용근(YK Yoo) 대한산부인과학회 1965 Obstetrics & Gynecology Science Vol.8 No.3

Determination of urinary estrogen is important to understand the physiology and diseases of the woman. Estrgens are present in the urine in conjugated form, mainly as glucosiduronate and ester sulfates. For the purpose of quantitative determination of urinary estrogen it is necessary to carry out an extensive concentration and purification. This is usually effected by acid or enzymatic hydrolysis followed by extraction of the liberated estrogens with organic solvents. But during the above procedures some loss of estrogen is inevitably followed. It seems to be very difficult to introduce any specific method which could completely prevent loss of the estrogen. Author deveolped a method which was relatively able to prevent loss of the estrogen and applied this to determination of estrogens of normal or abnormal women. Following results were obtained. 1) Acid hydrolysis with 15 vol. % HCI is more rapid and simple than enzyme hydrolysis. 2) The ether is a most suitable solvent for extraction of liberated estrogen by hydrolysis and carbonate buffer of pH 10.4 is used for purification of the other extracts. 3) The petroleum-ether is more adquate for the solvent of reextraction than ether and benzene. 4) Column chromatography is more simple than others. 5) For the colorimetry the hydroquinone-Kober method is more sensitive than Kober-Venning method. 6) Estrogen value for normally menstruating women is peak at secretory and ovulatory phases. Its mean value is 86.3 r/day. 7) Urinary estrogen value of amenorrheic women is relatively lower than that of normal women. Its mean value;63.0r/24hrs. Climacteric women also shows lower level than that seen in normal women. In seems therefore that in both amenorrhea and climacterium more estradiol (than normal women) is converted to estrone and estriol. 8) In castrated women mean estrogen value is 46.2r/24hrs. and estradiol-estrone conversion rate os decreased.

부신 및 난소에 있어서의 Steroid 홀몬 합성요소에 관한 연구

유용근(YK Yoo) 대한산부인과학회 1965 Obstetrics & Gynecology Science Vol.8 No.6

A diphosphopyridine nucleotide (DPN) dependent enzyme, and △5-3β-hydroxysteroid dehydrogenase can be easily demonstrated by biochemical procedure in adrenal gland Pregnenolone ovary of the rat. This enzyme converts the △5-3β-hydroxysteroids pregnenolone and dehydroepiandrosterone to progesterone and △4-androstenedione having the △4-3-ketone grouping characteristic of the active steroid hormones. This enzyme may be an important controlling factor in steroid hormone biosynthesis. Author developed a rapid, sensitive assay method for enzymatic activity of rat`s ovary by modifying Betty L. Rubin`s method of rat adrenal gland. Following results were obtained: 1) The optimal assay conditions for the detemination of ovarian enzymatic activity are similar as those of rat adrenal glands. 2) The enzymatic activities of ovaries and adrenal glands of adult rat are ovaries; sp. activity 8.0±0.5, total activity 8.2±0.2 adrenals; sp. activity 9.2±0.7, total activity 9.0±0.1 respectively. 3) A young rat`s ovary relatively greater specific activity and lower total activity than those of nomal adult rats. 4) After 3months of age, specific and total activities reached almost to the level seen in normal adult rats. 5) The influence of gonadotropin on enzyme systems of young and adult rats ovaries are different. In young rat, the increase of total activity is secondary effect of ovarian hyperplasia by stimulation of gonodotropin. Specific activity itself is not increased. But in adult rats, the increase in specific activity could be seen before ovaries markedly increased in seze, indicating that the increase in total activity and ovarian size are secondary effect of this specific activity. And it is, therefore, considered most probable that gonadotropin acts on enzyme already present. 6) Gooadotrapin did stimulate the 3β-OL-dehydrogenase of ovarian tissue slices in vitro by dehydrogenation. 7) It seems that the action of gonadotropin is not an specific reaction of steroid hormone biosynthesis but on a reaction supplying energy to a numbers of the biosynthetic enzyme systems. 8) ACTH did not stimulate the spectific activity in rat`s adrenal glands in vivo and vitro.

당뇨병과 임신

유용근(YK Yoo),박헌엽(HY Park) 대한산부인과학회 1963 Obstetrics & Gynecology Science Vol.6 No.6

이상 Prediabetic State를 경과한 후에 당뇨병울 병한 임신 36주의 임부를 입원 가료 하였기에 증예보고와 아울러 문헌적 고찰을 하였다.

임신반응으로서의 Histidine 및 Tryptopan 반응

유용근(YK Yoo) 대한산부인과학회 1961 Obstetrics & Gynecology Science Vol.4 No.3

1. 정상임부뇨에서는 histidine 뇨반응이 60% tryptophan 뇨반응이 78.5%의 적중율을 얻었고 임신 3개월이내에서는 histidine이 30%, tryptophan이 70%의 적중율로서 tryptophan이 histidine보다 임신과 더 밀접한 관계가 있음을 알았다. 2. 자간전증환자뇨에서는 histidine이 전예에서 음성반응을 보였다. 3. 병적임신뇨에서는 histidine 및 tryptophan이 전부 저율을 나타내었고 병적임신뇨에 대한 임신이상반응은 약 50%의 양성성적을 보았다. 4. 욕부뇨에 대한 histidine 및 tryptophan 뇨반응은 분만후 시일이 지날수록 양성율이 저하되어 적어도 분만후 1주일이내에는 거의 전예에서 음성화함을 볼 수 있었다. 5. 비임부뇨, 남성뇨에 대한 histidine 및 tryptophan 반응은 남녀간에 특별한 차이를 볼 수 없었다. 6. 이상의 성적으로 보아 histidine은 임신조기진단에는 이용가치가 없으며 오히려 임신시 물질대사의 변화를 관찰하는데 의의가 있고 tryptophan은 임신과 더 밀접한 관계가 있으나 임상적이용에는 앞으로의 연구가 필요하다고 생각한다. A simplified method for the identification of urinary histidine (Kapeller-Adler reaction), tryptophan and the color reactions for the derivative of its metabolite indol (Ashitaka reaction) was tested. The test was performed using a uring specimen from 120 normal pregnant women, 52 non-pregnant women, 40 male adults, 8 girls and boys, 30 puerpera, and 39 pregnancy abnormalities such as preeclampsia, ectopic gestation, abortion and premature delivery. Results: 1) Of 120 normal pregnant urine tests, there were 72 positive responses of histidine and 94 positive responses of tryptophan. In the first trimester of pregnancy the accuracies of 30% and 70% were obtained for histidine and tryptophan respectively. The increased excretions of urinary histidine were not found before the third month of pregnancy and cannot, therefore, be used as an aid in the early diagnosis of pregnancy. It was also experimentally shown that tryptophan was more closely related to pregnancy. 2) It was interesting that all cases of preeclampsia showed negative responses for histidine but 50% positive responses for tryptophan. 3) The histidine and tryptophan tests in the urine of pregnancy abnormalities showed low positive responses (about 50%) and pregnancy abnormality reaction was positive in about 50 percent. 4) In the test of puerperal women urine, the response became gradually negative from the second postpartum day and at least almost completely negative within the seventh postpartum day. 5) There was no detectable difference in histidine and tryptophan reaction between non-pregnant women and men.

처녀막폐쇄증으로 인한 질 및 자궁혈종을 동반한 1예

정용화(YW Chung),용호정(HC Bang),유용근(YK Yoo) 대한산부인과학회 1964 Obstetrics & Gynecology Science Vol.7 No.5

저자들은 15세 5개월난 처녀막폐쇄증환자로써 질혈종 자궁혈종을 일으켜 복부팽륭을 초래하여 당병원에서 처녀막 윤상절개를 행하여 경쾌퇴원한 1예를 보고하며 간단한 문헌적 고찰을 하였다. Imperforate hymen is a rare anomaly resulting in occult retention of the menstrual products within the genital tract. Its cause is divided in two parts largely, that is, congenital and acquired. One case of imperforate hymen regarded to congenital origin, and that formed hematocolpometra, observed on April 3rd, 1963 at the Dept. of Ob.-Gyn., Han-Il Hospital, is presented and the literature is reviewed.

흡입축만법에 대하여 ( 흡입분만, 흡인분만 )

강영호(YH Kang),박문선(MS Park),유용근(YK Yoo),서병준(BJ Seo) 대한산부인과학회 1963 Obstetrics & Gynecology Science Vol.6 No.3

The authors used Vacuum Extractor in 50 cases of deliveries at Han-II Hospital between May and August, 1962. 1) The indications for interference were; to shorten the period of 2nd stage and supplement for obstetrical anesthesia in 27 instances(54%), fetal distress in 5, prolonged 2nd stage in 4, toxemia 1, maternal disease 1, adruptio placentae 1, premature rupture of membrane 1, expulsion of a dead fetus 1. 2) Vacuum Extraction was successful in all the cases of multipara(24cases) and partial success in 4 cases of primipara(among 26 cases). We believe that the Vancuum Extractor has not yet reached the level of development neccessary to replace the obstetric forceps. 3) Maternal and fetal injuries were minimal. There were 5 cases of scalp adrasion, scalp avulsion 1 and abnormal enlargement of caput 2.

자궁파열의 2예

박문선(MS Park),강영호(YH Kang),방호정(HJ Bang),유용근(YK Yoo) 대한산부인과학회 1964 Obstetrics & Gynecology Science Vol.7 No.2

자궁파열의 2치험예를 보고함과 동시에 본증에 관한 문헌적 고찰을 하였다. Uterine rupture remains a serious threat to the life of the mother and a greater hazard to the survival of the unborn infant. Two cases of uterine rupture are presented. One case was spontaneous rupture of the uterine scar of the previous cesarean section at the thirtieth week of pregnancy. The other case was rupture of normal uterus during labor which was induced by oxytocic stimulation. The literatures on the uterine rupture are reviewed.