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산약의 기내증식에 (器內增殖) 미치는 배지와 생장조정제의 영향
이효승(Hyo Sung Lee),유수노(Su Nho Ryu),이정일(Jung Il Lee),조재연(Chae Yun Cho) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1
The low seed propagation is one of the problem needed a lot of seed tuber for the propagation in yam. Therefore this experiment was carried out to understand the possiblility of seed tuber propagation by tissue culture of yam. In-vitro stem node of yam was cultured by concentration treatments of 1/2, 1/4 and 1/8 with MS medium additted with each concentration levels of IAA, NAA, IBA, kinetin and BA. Acorrding to the Iower concentration than MS medium, length of shoots was promoted, leaf emergence shoots and rooting shoots were increased at 1/8 MS medium during the culturing period of stem node in yam. Fixed IBA and kinetin under the concentration of MS mdeium was inhibited severely by the heigh concentration additted with IAA 1㎎/ℓ and NAA 4㎎/ℓ. But fixed IBA 5㎎/ℓ and kinetin 2㎎/ℓ with concentration of 1/8MS medium was remarkably promoted leaf emergence shoots and rooting shoots by 1㎎/ℓ of additted IAA and NAA. Percentage of induced shoots was increased by combination treatments of IAA. 1.5㎎/ℓ and kinetin 2㎎/ℓ, also leaf emergence shoots and rooting shoots were promoted by combination treatments of IAA 1.5㎎/ℓ and kinetin 2㎎/ℓ.
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