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Islet Cell Surface Antibody ( ICSA ) 양성인 인슐린 비의존성 당뇨병 환자의 임상적인 특징과 ICSA 의 인슐린 분비억제에 대한 기전
우정택(Jeong Taek Woo),김덕윤(Deok Yoon Kim),김성운(Sung Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seol Kim),김광원(Kwang Won Kim),최영길(Young Kil Choi) 대한내과학회 1993 대한내과학회지 Vol.45 No.1
N/A glycemic control and significantly reduced insulin secretory response. 3) Inhibitory effect of serum immunoglobulins from the diabetics with ICSA on insulin secretion of p-cells induced by glucose stimulation was confirmed by the perifusion system. 4) The suggested mechanisms of ICSA on insulin secretion are ① inhibition of glucokinase and glucose transporter 2 mRNA expression ② inhibition of phosphoinositide hydrolysis metabolic pathway Conclusion: There is a certain group of adult onset diabetes still unclassified but characterized by appearance of ICSA, marked weight loss and markedly reduced insulin secretion and finally becoming insulin dependent. The ICSA detected in this group of adult onset diabetics may also play a role in an effect on multiple steps of signal transduction pathways related to insulin secretory mechanism.
99mTc - LDL ( Low Density Lipoprotein ) 신티그라피를 이용한 동맥경화병소 진단
김진우(Jin Woo Kim),김영설(Young Seol Kim),김광원(Kwang Won Kim),최영길(Young Kil Choi),고은미(Eun Mi Koh),김덕윤(Deog Yoon Kim),김성운(Sung Woon Kim),양인명(In Myung Yang),우정택(Jeong Taek Woo) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.2
N/A Diagnostic approaches such as angiography, ultrasound, computed tomography and nuclear magnetic resonance have limitation for contributing to the early clinical diagnosis of atherosclerosis. Recently, Tc-99m-labelled low density lipoprotein was developed to detect early atherosclerotic lesion by external imaging with gamma camera. To determine whether Tc-99m- LDL scintigraphy can visualize the active atherosclerotic lesion, rabbits were injected with Tc- 99m-LDL, 3 months after feeding dietary fat (lanolin) and we obtained following results. 1) Labelling efficiency of Tc-99m-LDL was 79∼88%. 2) Biodistribution study of normal rabbits with Tc-99m-LDL revealed the high activities in spleen, adrenal gland, liver, kidney which are major organs of high metabolic rate of LDL. 3) Three months after feeding lanolin, serum cholesterol was markedly increased from 74±17 mg/dl to 979±153 mg/dl and histologic study of aorta after sacrificing the rabbit demonstrated marked atherosclerotic changes. 4) Atherosclerotic lesion of abdominal aorta which was confirmed with histologic study could be demonstrated in Tc-99m-LDL scintigraphy after feeding lanolin for 3 months. In conclusion, the results of this preliminary investigation suggest that it may be possible to image active atheromatous lesion with Tc-99m-LDL. It is anticipated that this promising agent may allow the in vive monitoring of preclinical atherosclerotic lesions and may be useful to evaluate the metabolic path way of LDL in humans.
HIT 세포에서의 Dexmethasone 이 글루코키나제와 글루코기나제 mRNA 발현에 미치는 영향
안규정(Kyu Jeong Ahn),김덕윤(Deok Yoon Kim),우정택(Jeong Taek Woo),김성운(Sung Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seol Kim),김광원(Kwang Won Kim),최영길(Young Kil Choi) 대한내과학회 1994 대한내과학회지 Vol.46 No.4
N/A Objectives: The glucokinase-glucose sensor concept of physiological glucose recognition by pancreatic beta- cells has developed progressively since the presence of this enzyme in beta-cells was first reported in 1968. It is well known that HIT cells have glucokinase activity and then insulin secretory response according to glucose stimulation, but there are few data about the effect of hormone on insulin secretion and glucokinase in HIT cells. Therefore, we studied the effect of dexamethasone on glucokinase activity, mRNA expression and insulin secretion in HIT cells. Methods: HIT cells were cultured for 24 hours in medium containing 0, 50, or 500 nM dexamethasone, and then the activities of glucokinase was quantitiated using pyridine uncleotide-dependent fluorometric assays. Northern blot analysis of glucokinase mRNS was done. Results: 1) The Vmax and Km of glucokinase in HIT cells were 3.5 μ mol/min/mg protein, 0.77 mM in HIT cells exposed to 7 mM glucose, respectively and the Vmax and Km of hexokinase in HIT cells were 2.64 μmol/ min/mg protein, 0.06 mM, respectively. 2) 24 hours after incubation of HIT cell with dexamethasone 0, 50, 500 mM, the glucokinase activity and insulin secretion were observed: the glucokinase activities were 1.4±0.45(control), 0.87±0.43(50nmol dexarnethasone), 0.13±0.52 uU/mg protein(500 nmol dexamethasone), and insulin secretion were 44.57±4.1(control), 35.50±1.72(50 nmol dexamethasone), 31.62±1.07 ng/mg protein(500 nmol dexamethasone), respectively. 3) Northern analysis revealed that dexamethaxone increase glucokinase mRNA expression according to dexamethasone concentrations 24 hours after incubation of HIT cells with dexamethasone 50 or 500 nM. Conclusion: Our data showed that dexamethasone tended to increase glucokinase mRNS expression, but decrease glucokinase activity and insulin secretion in HIT cells. Thease data suggested that dexamethasone may have an variable effect on transcriptional or posttranslational levels of glucokinase.
인슐린 비의존형 당뇨병에서 글류코키나제 유전자 변이에 대한 연구
오승준(Seung Joon Oh),우정택(Jeong Taek Woo),김덕윤(Deok Yoon Kim),양인명(In Myung Yang),김성운(Sung Woon Kim),김진우(Jin Woo Kim),김영설(Young Seol Kim),최영길(Young Kil Choi),팽정령(Jeong Ryung Paeng) 대한내과학회 1996 대한내과학회지 Vol.50 No.3
N/A Objectives : Considerable evidences suggest that genetic factors are of crucial importance in the pathogenesis of NIDDM. As glucokinase is a major enzyme of glucose homeostasis, the glucokinase gene has been proposed as one of candidate genes that confer genetic susceptibility to NIDDM. Although recent studies suggest that mutations of the glucokinase gene related to MODY, a possible relationship between this gene mutation and other subtypes of N1DDM has not investigated. Methods: To investigate the possible relationship between the mutations and NIDDM in Koreans, we screened mutation over all 12 exons of glucokinase gene in 30 normal controls (group 1) and 103 NIDDM patients, 34 patients without family history (group 2) and 69 patients with family history (group 3), by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism). Results : Eighteen of 133 subjects (13.5%) had mutations which were found in exon 10β, 1L, 1V, and 5, Four different types of polymorphisms were found in exon 10β, 3 types in exon 1L, 1 in both exon 1V and exon 5. Seventeen of 103 NIDDM patients (16.5%) and 1 of 30 normal control (3.3%) had mutations, which suggests that the mutations are related to NIDDM. The frequency of mutations was not different between group 2 and 3. However, the frequency of mutations was higher in the patients with low 24 hour urine C-peptide excretion than those who not. Serum glucose concentration was significantly higher and serum insulin level tended to decrease at 60 min after oral glucose loading in the patients with mutations. Conclusion: These data suggest that the mutations of glucokinase gene are related to NIDDM in Koreans, especially who have low insulin secretory capacity. Further studies are needed to clarify how the mutations relate to NIDDM.
당뇨유발 마우스에서의 hollow fiber 로 캡슐화한 췌장소도의 이종이식
이무열(Moo Yeol Lee),우정택(Jeong Taek Woo),김성운(Sung Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seol Kim),김광원(Kwang Won Kim),최영길(Young Kil Choi),서광식(Kwang Sik Suh) 대한내과학회 1994 대한내과학회지 Vol.46 No.3
N/A Background: We have investigated whether xenograft of the pancreatic islets encapsulated in hollow fiber (Amicon, H1P 30~43 type) could normalize blood glucose levels and could secrete insulin normally in perifusion system. Method: Mice (ICR) made diabetic with 180 mg/kg streptozotocin were intraperitoneally transplanted with encapsulated rat pancreatic islets, Hollow fibers (Amicon, H1P30~43 type, nominal cutoff MW; 30,000) have been used for encapsulation of rat islet cells. Result: Rat islets in the hollow fibers secreted insulin normaly in perifusion system. Xenograft of rat islets in the hollow fibers produced and maintained temporarily normoglycemia in the recipient mice. Conclusion: These results suggest that xenograft of rat islets in the hollow fibers need further study for biocompatability, transplantation site, and islet cell counts.