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Synthetic nucleus mimicry for a mRNA production
엄숭호,신승원 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Since several decades, many studies for mimicry of a living organism have been done owing to its distinctive. Yet, few have been attempted to replicate the unique properties. Here we first present a cell’s nucleus with the ability of a transcript decoder for an enhanced mRNA level. It is simply created by enzymatically crosslinking a gene with DNA origami crosslinkers resembling a histone as a gene holder. After further treatment with lipid bilayers and successive transcription factors, it effectively promoted the expression of a target RNA transcript. In comparison with other control groups (e.g., ones without either a gene or a crosslinker), it showed much stronger emission of encoding RNAs tested. Once internalized by target cell (e.g., MCF-7), it ensued a higher and longer expression of GFPs. It successfully replaced the older and restored the system. The artificial cell nucleus mimicry may be a new archetype for advanced gene therapy.
Observation of a mouse sperm motility in a natural uterine tube-inspired microfluidic channel
엄숭호 한국바이오칩학회 2013 BioChip Journal Vol.7 No.1
Bio-inspired engineering has been promisingly spotlighted recently due to the replication of unique natural environments and processes. Fertilization occurs when sperm meets an egg, typically within the uterine tube. Using bio-inspired engineering, we developed natural uterine mimicry for studying sperm motility via the synthetic microfluidic tube system. Mature sperm were collected from the testicular cauda epididymis, and subsequently remained stable for up to 2 days. The primary sperm cells were infused into a single 1.5 cm wrinkle wave patterned microfluidic channel, moving in a prototypical forward sine wave pattern. This study may be useful in understanding the natural behavior of sperm and also may be applied to fertility treatments in the future.
Gel Electrophoretic Mobility Evaluation of a Necklace-like DNA Nanostructure
엄숭호 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
DNA nanotechnologies have been highlighted as a promising synthetic tool for the creation of new shaped materials. They have developed a variety of materials in different shapes and sizes [1]. Inspired by these advancements, we sought to design a ring-shaped DNA nanostructure connected by X-DNA blocks. Six XDNA blocks were ligated together to form a circular nanostructure with a diameter of approximately 30 nm. Each DNA block possesses different overhang sequences in its terminal. It was sequentially built up onto each block platform in the line and later clipped into a necklace shape via enzymatic ligation. It was finally evaluated by a gel electrophoretic migration shift assay. It was concluded that the complete set of the necklace shaped DNA nanostructure was the most slowly retarded relative to other forms of incompleteness.