http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 양덕천 (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
인삼 모상근의 캘러스를 이용한 ginsenoside 생산
권정희(Jung-Hee Kwon),천현준(Hyun-Choon Cheon),양덕조(Deok-Cho Yang) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.2
By the Agrobacterium rhizogenes A₄were induced a transformed callus of ginseng hairy root and examine to find the possibility whether it can produce certain ginsenoside. Investigations for a finding out to optimal culture medium showed that BA application is better than more factorial composition between auxins and cytokinins. For the induction of hairy root callus of ginseng, 1/2 MS medium containing 1 to 3 mg of benzyladenine(BA) per liter gave the best result. The growth of ginseng hairy root callus(GHC) cultured with the 1/2MS medium supplemented with 2 mg BA/L was<br/> selected for best suspension cultures. The optimum concentration of BA for ginsenosides production was found to be 2 mg/L. Probably the inoculum size of callus plays a role with the ginsenoside production in suspension culture. AS for inoculum size of callus, 50 mg was superior to 150 mg for growth and ginsenoside production. Ginsenoside contents were highest in the suspension culture grown for four weeks under continuous light condition. In fact that continous light treatment promote strongly the synthesis of ginsenoside of the hairy root callus is first result in the world and the numerously induced root hairs of the callus leads a new method for ginsenoside production.
-
Padmanaban Mohanan,허준,김선오,나주련,김유진,이현아,김민경,양덕천 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3
The traditional oriental medicine bokbunja, prepared from immature berries of Rubus coreanus is used as an anti-oxidant, diuretic, and cure for impotence. The bokbunja wine made from fermented fruits of bokbunja has been used as a functional food as well. However, the usage of bokbunja has been problematic over the years due to the abundance of mistakenly identified berries such as Rubus chingii, Rubus crataegifolius, and Rubus occidentalis. Thus, here we developed a method for the molecular differentiation of Rubus species as well as the authentication bokbunja from other Rubus species. We screened several sequences from the chloroplast DNA of these species and found that the rpl16 region was polymorphic for R. coreanus and R. occidentalis, while the trnG–trnS intergenic spacer region was polymorphic for R. chingii and R. crataegifolius. Species-specific primers were designed and a multiplex PCR was performed by combining the markers at the rpl16 and trnG–trnS regions. Amplicons of 686 bp for R. coreanus and 478 bp for R. occidentalis were produced by the primers 5′ Rcor or 5′ Rocci, respectively, with 3′ rpl16; whereas, amplicons of 389 bp for Rubus crataegifolius and 180 bp for R. chingii were produced by 5′ Rcra or 5′ trnG–trnS, respectively, and 3′ Rcra/Rchi. The deduced molecular markers were utilized to authenticate the bokbunja products and demonstrated that the majority of bokbunja samples from the markets were adulterant berries. Hence, our results indicate that the produced molecular markers can serve as an effective tool to authenticate bokbunja.
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
