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김재훈,손현주,황의경,황우석,허권,진영화,이병천,이재진,강영배,야마네 이츠로,김대용,Kim, Jae-hoon,Sohn, Hyun-joo,Hwang, Eui-kyung,Hwang, Woo-suk,Hur, Kwon,Jean, Young-hwa,Lee, Byung-chun,Rhee, Jae-chin,Kang, Yung-bai,Yamane, Itsuro,Kim, 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.
간접형광항체법을 이용한 국내 젖소의 Neospora caninum에 대한 혈청역학적 연구
허권,김재훈,황우석,황의경,진영화,이병천,배지선,강영배,야마네 이츠로,김대용,Hur, Kwon,Kim, Jae-hoon,Hwang, Woo-suk,Hwang, Eui-kyung,Jean, Young-hwa,Lee, Byung-chun,Bae, Ji-seon,Kang, Yung-bai,Yamane, Itsuro,Kim, Dae-yong 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4
This study was carried out to investigate the prevalence of Neopsora (N) caninum infection in Korean dairy herds. To determine the prevalence of antibodies to N caninum in Korean dairy cattle, a total of 1,688 sera including 895 sera taken from 30 herds having recent high abortion rate and 793 sera selected randomly from 168 herds with no history of recent abortion problem, respectively, collected nationwide during a designated period were analyzed by indirect fluorescent antibody (IFA) test. Mean nationwide seropositive rate of the sera tested in herds and individual cattle tested were 53.5% and 35.6%, respectively. However mean seropositive rate of the samples from herds having abortion problem was approximately two and half times higher than those in herds with no recent abortion history. Regional seropositive rates of the samples from the herds with abortion problem were 48.6%, 51.6%, 44.4% and 71.4% at Kyunggi, Kangwon, Kyungbuk and Jeonnam province, respetively. Regional seropositive rates of the samples from the herds with no recent abortion problem were 35.6%, 18.3%, 16.5%, 37.5%, 19.4%, 33.3%, 32.1%, 3.8% and 0.0% at Kyunggi, Kangwon, Chungbuk, Chungnam, Kyungbuk, Kyungnam, Jeonbuk, Jeonnam and Jeju province, respectively. The results of this study suggested that N caninum infection was widespread and considerably associated with bovine abortion in Korea.