http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김석창,안건석,박채규,전병선,이종태,박원종 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.4
황금 (Scutellaria baicalensis Gergi)에 함유된 항산화 억제활성 물질을 분리하고 항산화 효과에 대하여 조사한 결과는 다음과 같다. 1. 황금 500g을 100% MeOH로 추출하여 150g의 수율을 얻었으며, 다시 분획하여 Ethyl ether 13.2g과 n-BuOH 10.3g, Water 126.5g을 얻었다. 2. Ethyl ether 분획을 Column chromatography, TLC 및 HPLC를 이용하여 xanthine oxidase 억제활성을 가진 compound I을 분리하였다. 3. 1H-NMR, 13C-NMR, MS spectrometer 등 분광학적 방법을 이용하여 Compound I의 구조를 규명한 결과, flavonoid 물질로서 compound I은 구조식이 3,5,7-trihydroxy-2'-methoxy-flavanone로 확인되었다. Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of 0.1 μg. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by 1H-NMR, 13C-NMR and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration 100 μg/3 ml. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using 1H-NMR, 13C-NMR and Mass spectrophotometry.