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- 저자 : 아카키타다쯔(Tadaatsu Akagi) (검색결과 1 건)
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HTLV - I 의 불멸화에 의한 T 세포의 특성 및 한국인 악성 림프종과 Retrovirus 감염과의 관계
전호종(Ho Jong Jeon),서재홍(Chae Hong Suh),기근홍(Keun Hong Kee),김윤신(Yun Sin Kim),이미숙(Mi Sook Lee),정춘해(Choon Hae Chung),정종훈(Jong Hoon Jung),박영진(Young Jin Park),김향우(Hyang Woo Kim),아카키타다쯔(Tadaatsu Akagi) 대한내과학회 1995 대한내과학회지 Vol.49 No.3
N/A Objectives: 1) The relationship between malignant T-cell lymphoma and retroviral infection was examined in sixty-two cases of non Hodgkin's lymphoma arising in nodal and extranodal site to presume the etiologic factors in the pathogenesis of T-cell lymphoma arising in Korean. 2) Human T-cell leukemia/lymphoma virus type I(HTLV-1) is known as an etiologic agent of adult T-cell leukemia/lymphoma(ATL), however, the precise role of the HTLV I on the genesis of ATL is unclear. To elucidate the mechanism of the leukemogenesis on the ATL, establishment of cell line harboring HTLV-I is indispensable. Methods: 1) Sixty- two cases of malignant lymphoma were examined with a battery of monoclonal and polyclonal antibodies directed to T-cell, B-cell and macrophage-lineage to determine the immunophenotypes. Reverse transcriptase activity and the pol gene region in HTLV-I were examined by RNA directed DNA polymerase assay and polymerase chain reaction. 2) Two helper T-cell lines, designated JP(JP-1 and JP-2), were established by co-culturing normal human cord leukocytes with a lethally irradiated HTLV-I-harboring human leukocytes cell line(MT-2). Results: 1) There are no evidence of retroviral infection including HTI.V-I in examined 7 cases of peripheral T-cell lymphama. 2) JP-1(interleukin 2 dependent) had a normal karyotype, and expressed the surface markers CD3+, CD4+, CD8-, CD19-, CD25+ and HLA-DR+. JP-2(IL-2 independent) had tetraploidy on the 22nd passage, and exhibited the surface markers CD3-, CD4+, CD8-, CD19-, CD25+ and HLA-DR+. T-cell receptor(TCR)-β chain gene was detected in these two cell lines by Southern blot hybridization techniques with (32)P-labelled DNA probe Jpconfirming T-cell nature. Jβ2 cells were all immunoreactive with anti-HTLV-1, p 19, p 24, gp46 and pX antibodies. The proviral genome of HTLV-1 was detected in these cell lines by Southern blot hybridization by the (32)P-labelled DNA probe, pHT-1 (M)3.9. Electron microscopy of JP 2 cells revealed a few of type C virus particles. Conelusion: 1) These results suggest that the evidence of retroviral infection as an etiological factors in the pathogenesis of T-cell lymphoma is poor. 2) HTLV-1 was transmitted from the infected human leukocyte cell line to human cord helper T-cells with the development of immortalized HTLV-1 producing T-cell lines, and IL-2 independent cell line(JP-2) lost their CD3 antigen, It is suspicious that there is the second factors to induce malignant transformation in the genesis of adult T-cell leukemia/lymphoma because of absence of the tumorigenecity in the JP cell lines harboring HTLV-1.
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