식물병원성세균의 약제저항성에 관한 연구 III. 벼 흰빛잎마름병균에 있어서 Streptomycin 내성균주의 몇 가지 변이

조원철,심재욱,Cho W. C.,Shim J. W. 한국응용곤충학회 1977 한국식물보호학회지 Vol.16 No.4

선발에 의하여 얻은 벼 흰빛잎마름병균 Xanthomonas oryzae의 Streptomycin 내성균에 대하여 병원성, 배지내에서의 생장량 및 자외선 감수성에 대한 변이성을 조사하여 다음과 같은 결과를 얻었다. (1) SM 내성균은 Wase-Aikoku-3, Rant Emas-2 황옥 및 Kimmase의 4개 판별품종에 대한 병원성 비교에서 75-6의 내성균주가 황옥에 대하여 감수성 반응이 모균과 달리 중정도의 저항성을 나타내었다. 2) SM-내성균은 정상배지에서 모균보다 약간 높은 생장량을 보였고 100ug/ml Agrepto 첨가 배지에서는 접종후 60시간까지 생장이 조지되었으나 70시간 이후에는 모균의 생장량을 능가하였다. (3) SM-내성균은 254mu 파장의 자외선 조사에서 모균과 동일한 감수성을 나타내어 내성인자는 안정된 것으로 생각되었다. The experiments were contucted to investigate the variability of pathogenesity, growth amount and UV-sensitibity on the streptomycin resistant isolate of the rice bacterial leaf blight pathogen, Xanthomonas oryzae, which selected by the stepwise transfer in 100, 3,000 and 10,000 ug/ml Argepto contained media. And the results obtained were as follows. (1) The SM-resistant isolates were tested the pathogenesity on the differential variety of rice, wase-Aikoku-3, Rant Emas-2, Hwang ok, and Kimmase. And the SM resistant isolate, obtained from 75-6 isolate, showed the reaction of moderately resistant to the differential variety of Hwang ok instead of susceptible reaction with its parental isolate. (2) The growth amount of the SM-resistant isolate was slightly higher than that of parental isolate, on the normal media. And the growth was inhibited on the 100ug/ml Agrepto contained media until 60 hours after transfer, however, its growth exceeded than parental isolate in the normal media, after 70 hours of transfer. (3) It is considered that the resistance factor might be stable character, since the sensitivity to UV irradiation (with 254 mr wave length) of the resistant isolate was the same as that of its parental isolate.

식물병원성 세균의 약제저항성에 관한 연구 II. 벼흰빛잎마름병균의 Agrepto에 대한 저항성의 선발효과

조원철,심재욱,Cho W. C.,Shim J. W. 한국응용곤충학회 1977 한국식물보호학회지 Vol.16 No.4

벼 흰빛잎마름균을 이용하여 Streptomycin 계약제인 Agrepto에 대한 내성형질의 선발효과를 조사하여 다음과 같은 결과를 얻었다. 1) 사용된 약제 Agrepto는 $105^{\circ}C$에서 20분간의 온도처리에서 약제의 안정성을 보였다. 2) 서로 다른 지역균주인 75-6 및 75-9 묘주는 선발영제농도 10ug/ml 및 100ug/ml에서 상이한 내성력을 나타내는 내성균이 선발되었다. 3) 저농도인 10ug/ml 및 100ug/ml의 선발농도에서도 10,000ug/ml에서 생장이 가능한 내성균이 선발될 수 있었다. 4) 전세대 100ug/ml에서 선발하고 재차 3,000ug/ml에서 선발된 선발균주는 처리농도 100ug/ml에서 무처리와 동일한 생장량을 보였다. 5) 자연집단의 균주 75-9를 100ug/ml에서 선발한 이들 분리균주는 내성 정도에 있어서 500ug/ml 이하, 500-1,000ug/ml 및 1,000-3,000ug/ml의 내성을 나타내는 몇단계의 내성력 정도가 상이한 균들이 분포하고 있었다. The experiments were carried out to investigate the selection effects on the drug resistance to Agrepto in Xanthomonas oryzae, the causal bacteria of rice bacterial leaf blight. The results obtained were as follows. 1, The Agrepto was stable at the heat treatment of $105^{\circ}C$ for 20 minutes, when the drug was added in the media. 2. The local isolates of the bacteria, 75-6 and 75-9, showed the different resistante reaction, when they were selected by the Agrepto contained media which concentrations of 10r9/m1 and 100ug/ml. 3. The individuals shelving high degree of resistance, which can grow on the media contained 10,000ug/m1 of Agrepto, could selected by the concentrations as low as 10ug/ml and 100ug/m1, in one generation. 4. The highly resistant isolates which selected by 3-stepwise selections such as 100ug/ml, 3,000ug/ml and 10,000/ml plots, showed nearly normal growth at the media contained 100ug/ml of Agrepto 5. When the isolate 75-9 was selected at the 100ug/m1 of concentration, showed various degrees of resistance, indicating that the isolate may be composed of resistance groups that lower than 500ug/ml, between 500-1,000ug/ml and 1,000-3,000ug/ml, to the Agrept.

솔잎혹파리 발생예찰을 위한 우화기구개발에 관한 연구 I. 우화기구별 솔잎혹파리 보호효과

우건석,심재욱,Woo K. S.,Shim J. W. 한국응용곤충학회 1978 한국식물보호학회지 Vol.17 No.2

본 실험은 솔잎혹파리의 성충우화수 조사기구를 개발하기 위하여 경기도 안양시 안양리 산 160번지와 수원시 서울대학교 농과대학에서 실시하여 다음과 같은 결과를 얻었다. 1. 솔잎혹파리의 우화기간은 5월 중순부터 8월중순이었고 우화최성기는 기구별로 차이가 있어 표준형은 5월 29일-6월 2일, Funnel-A형은 6월 3일-7일, Suction형 Trap은 6월 8일-12일간이었다. 2. 우화율은 실내실험구의 Funnel-A형에서 $41.06\%$였다. 3. Suction형 Trap은 밀도가 낮은 우화초기와 말기에 보호효과가 현저히 우수하였다. 4. 기존 표준우화상의 결점을 보완하기 위해 Funnel-A형과 Suction형 trap의 개발이 바람직하다. The experiments were carried out both at $#160$, Anyang Ri, Anyang, Kyung-gi Do, as a field plot and at college of Agriculture, Seoul National University, Suweon as laboratory plot, in order to unnorive the types of traps for collection of adult pine gall midge (Thecodiplosis japonensis Uchida) And the conclusions of the experimental results were as follows. (1) The seasonal fluctuation of the adult emergence of the PGM continued from middle pa.1 of the May to late part of the August. And the peak of the adult emergence showed variation in the number of PGM captured, according to the types of traps used, such as May 2g-june 2, by standard Cage, June 3-June7 by Funnel-A t.sp and June 8-June 12 by Suction-Trap. (2) The Percentage of the emergence in the laboratory experimental Plot were $41.06\%$ of Funnel-A traps used. f 3) The Suction-Trap was considered as effective instrument of. collection of adult PGM especially in the seasons of both earlier and later parts of the adult emergence, which is the population density is extremelty low. (4) It is considered that the recommendable instruments were Funnel-A type of trap and suction-Trap according to the experimental results obtained.

솔잎혹파리의 성충밀도를 줄이기 위한 점착물질의 이용에 관한 연구

우건석,심재욱,Woo K.S.,Shim J.W. 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.4

본 시험은 점착물질을 이용하여 솔잎혹파리 우화성충의 밀도를 감소시키기 위해 지면산포, 지피식생 및 수간산포의 세 방법으로 이 시험을 위해 조제한 점착제 $C_4$를 이용하여 경기도 안양시 산본리에서 시기별로 시험한 결과는 다음과 같다. 1. 점착제로 선정된 $C_4$의 조성은 피마자유 $70\%$, Damar 수지 $25\%$, Carnauba Wax $5\%$였으며 점성, 점도의 지속성이 우수하였고, 약해가 비교적 낮았다. 2. 지면산포구에서 우화최성기의 성충포획수는 대조구에 비해 G-II구는 $78.0\%$가 감소 되었다. 3. 지피식생산포구에서는 우화최성기의 성충포획 수는 대조구에 비하여 P-I구는 $63\%$, P-II구는 약 $90\%$가 감소되었다. 4. 수간산포구에서는 충영형성율이 F-I구가 $32.8\%$ F-II구는 $40.8\%$, F-II구는 $59.4\%$로 처리시기가 빠를수록 충영형성율이 낮았으나 F-I구는 F-II구간에는 유의차가 없었으며 소나무의 상${\cdot}$중${\cdot}$하부위별 충영형성율은 유의차가 없었다. 5. 점착제의 산포시기는 전처리구에서 우화최성일 1주일전이 효과적이었다. The present experiments were carried out to reduce the population density of adult pine gall midge, Thecodiplosis japonensi,s UCHIDA et INOUYE, by means of spray the sticky agent C-4, on the ground, herbacious plantation and foliar leaf of pine trees at Sanbonli,. Anyang, Kyungido. And also the 3 periods of treatment, such as before 2 weeks( I ), before 1 week (II) from the peak emergence period and peak emergence period (III) were applied. The experimental results obtained were as follows. (1) The formula of selected sticky agent C-4 was $70\%$ of castor oil, $25\%$ of damar resin and $5\%$ of carnauba wax, and it showed the best both on stickiness and duration, and lower phytotoxicity to the host plant. (2) The reduction of population densities of the adult PGM were $68\%$ and 78f: in the G-I and G-II treatment plot respectively, which compared to control, on the ground spray. (3) And the reduction of population densities were $63\%$ and about $90\%$ in the P-I and P-II plot respectively when the agent was sprayed on the herbacious plantation. (4) The rates of gall formation were $32.8\%,\;40.8\%\;and\;59.4\%$ in the spray plots of F-I, F-II, and F-III respectively, and there was no significant difference among the upper, middle and lower parts of the treated host plant in the rates of gall formation. (5) The effective stage of sticky agent application were considered as before one week from the peak emergence period in tile all types of treatment.

토양병의 발병억제 기작과 그 실용성 길항성 Trichoderma spp. 의 분리 , 동정 및 근권내 활동 길항성 Trichoderma spp. 의 분리 , 동정 및 근권내 활동

김성일,심재욱,신현성,최혜정,이민웅 ( S . I . Kim,J . O . Shim,H . S . Shin,H . J . Choi,M . W . Lee ) 한국균학회 1992 韓國菌學會誌 Vol.20 No.4

솔잎혹파리 발생예찰을 위한 우화기구 개발에 관한 연구 II. 우화기구별온도효과가 우화소장에 미치는 영향

오명희,우건석,심재욱,Oh M.H.,Woo K.S.,Shim J.W. 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.3

This experiments were performed to investigate the temperature effects, which caused by the various types of collecting trap treatments, during the overwintering period of pine gall midge larvae, on the peak adult emergence time. And the following results were obtained. 1) The maximum temperatures inside of the collection traps were significantly different each other, and the vinyl cage showed the highest degree which highering $15^{\circ}C$, and standard cage, cage-50 and funnel trap highering $3-12^{\circ}C$ in monthly average comparing to control. 2) There was no significant difference among the treatment in minimum temperatures, during the overwintering periods of larvae. 9) The peak adult emergence time were May 15 in vinyl cage plot which shortened 20 days and both standard cage and cage-50 shortened 14 day than that of control. 4) The funnel type trap treatment delayed 6 days of the peak adult emergence comparing to control, in spite of the treatment showing higher maximum and cumulative temperatures than the control plot. 5) The decadal maximum temperatures of middle part of December and March were significantly correlated with the shortening of the peak adult emergence date. 본 실험은 몇가지 우화기구를 사용하여 기구형에 따른 우화상내 온도변화가 솔잎혹파리의 우화소장에 미치는 영향 및 유충의 월동기간중의 온도변화와 우화소장간의 관계를 알아 보기 위하여 각처리별 우화소장을 조사하여 다음과 같은 결과를 얻었다. 1) 우화기구별 상내 최고온도는 현저한 차리를 나타내어 대조구에 비하여 Vinyl형은 월평균 $15^{\circ}C$가 높았고 다른 처리구에 있어서도 $30^{\circ}C\~12^{\circ}C$가 높았는데 다른 처리구에 있어서도 $30^{\circ}C\~12^{\circ}C$가 높았는데 표준형의 순이었다. 2) 처리별유충의 월동기간중의 최저온도분포는 현저한 차이를 나타내지 않았다. 3) Vinyl형 처리구에서는 우화최성일이 5월 15로 대조구에 비하여 20일 앞당겨했고 표준형구와 Cage-50구도 14일 빨라졌다. 4) Funnel형 처리구에 있어서는 최고온도 및 적산온도의 분포가 대조구에 비하여 높았음에도 불구하고 우화최성일은 6일이 지연되었다. 5) 유충의 월동기간중의 온도처리효과는 12월중순과 3월중순의 최고온도의 상승이 우화최성일을 앞당기는 효과를 나타내었다.

韓國에 있어서 小麥 半矮性遺傳子의 起湲, 傳播 및 利用에 關한 硏究

C.H. CHO(曺章煥),B.H. HONG(洪丙憙),M.W. PARK(朴文雄),J.W. SHIM(沈載昱),B.K. KIM(金鳳九) 한국육종학회 1980 한국육종학회지 Vol.12 No.1

Origin, dessemination and utilization of semi-dwarf genes of wheat were studied. Begining of wheat cultivation in Korea was about 3000 years ago and varietal name was given around in 1400 A.D. Original population carrying semi-dwarf genes was found to be Korean native Anzunbaengimil or Nanjangmil which was introduced to Jappan in the period of 1904~1905 and presumably renamed as Daruma. Genetic resemblance of these two populations was discussed. Number of pure-line selections were made from this population and Norin 10 was released in Japan by utilizing this semi-dwarf genes. Korea also released Suweon 92 and Seuseun 27 using same gene sources. Contribution of this semi-dwarf genes to the increase of world wheat production was reviewed. Semi-dwarfing genes of wheat which extended a great contribution for increasing wheat production in the world were investigated on their origin, dissemination, and utilization and the results were summarized as followings. 1. Begining of wheat cultivation in Korea was postulated to be about 3000 years ago considering the history of wheat cultivation of China and Japan. 2. Wheat varietal name was recorded as early as in 1482. However, Nongsajiksul published in 1400 has a brief description about wheat (cultural) methods. Therefore, it is not difficult to presume that varietal name was available to that time. 3. Original population where the semi-dwarf plants were selected were Anzunbaengimil or Nanjangmil which were distributed all where in Korea in the period of 1500~1941. This population had great variation in plant height, presumably a mixture with individuals carrying Rht₁, Rht₂ or Rht₁ Rht₂. 4. Dissemination of Anzunbaengimil to Japan was possiblly made during the period of Imjin Japanese Invasion to Korea, or by the Japanese Agronomists who were dispatched to Korea for investigation of agriculture and land productivity from 1901 to 1905. 5. Anzunbaengimil(Crippled wheat) may be named as Daruma(crippled wheat) after introduction to Japan or renamed as Daruma after pure-line selections were made. Considering such traits as growth habit, glume color, plant, height grain color and plant type it was concluded that Daruma is orginated from Auzunbaengimil population. 6. In Japan, semi-dwarf gene from Daruma population have been utilized extensively and released Norm 10 which became an important world wide breeding material. Seuseun 27 and Suweon 92, the Korean semi-dwarfs, were also improved using same dwarf gene source as Norin 10. 7. Dwarf genes from Daruma population, have contributed for improving several early maturing and high yielding wheat varieties in Korea such as Suweon 85, Chokwang, Suweon 215, 216, 219 and 220. 8. Norin l0/Brfvor 14 was the dwarf gene source for improving Cains and Nugains at Washington State, USA and CIMMYT semi-dwarfs. Coullee, a hard white winter wheat, was improved using Suweon 92, and Seuseun 27 was utilized for improving Tam Wheat and Holley wheats at Texas A&M, USA.

제2부 제초제 및 잡초방제 분야 : 미생물 제초제원 선발 및 미생물 배양액의 제초활성에 관한 연구

김병섭(B . S . Kim),조광연(K . Y . Cho),김진석(J . S . Kim),최정섭(J . S . Choi),이민웅(M . W . Lee),심재욱(J . O . Shim),이윤수(Y . S . Lee) 한국잡초학회 1997 한국잡초학회 별책(학술대회 초록집) Vol.17 No.1

自殖性作物에 있어서 Isogenic line과 Multiline의 育成과 利用

C.H. CHO(曺章換),K.W. CHUNG(鄭吉雄),J.W. SHIM(沈載昱),D.J. MAENG(孟敦在) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

麥類의 屬間交雜에 關한 硏究 IV. 8倍體 Primary Triticale의 細胞學的 硏究

W. S. AHN(安完植),C. H. CHO(曺章煥),H. B. KIM(金興培),J. W. SHIM(沈載昱) 한국육종학회 1979 한국육종학회지 Vol.11 No.1

In the present investigation, cytological and genetical research on Primary Triticale hybrids between Triticum aestivum (Jugoku #81) and Secale cereale (Jaelae) was carried out. Fifty six chromosomes were observed in both mitotic and meiotic cells of Primary Triticale. The chromosomal behaviour of both mitotic and meiotic cells were more regular than that of F₁’s, especially in meiotic division. However, 79.1% of the meiotic cells had from 1 to 8 univalents.