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Randomly Amplified Polymorphic DNA (RAPD) 기술을 이용한 고려인삼의 유전분석을 위한 Primer 선발 및 변종별 비교
임용표,신최순,이석종,윤영남,조재성 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
The study was carried out for comparison of variants and development of genetic markers using Randomly Amplified Polymorphic DNA(RAPD) analysis method. The ginseng variants used were as follows; Chungkyung-Chong. Hwangskoog-Chong, KG101 selected by the pure-line selection method, and 6 kinds of Jakyung-Chong strains (Jinjakyung, Jakyung-Chong 81783, Jakyung-Chong 847913, Jakyung-Chong 79742, Jinjakyung of USSR, and Mimaki of Japan). Four of 10 RAPD primers showd the distinctive polymorphism among 9 ginseng variants and lines, and were selected for more detailed polymorphic analysis. The sequences of 4 selected primers were TGCCGAGCTG (primer#2), AATCGGGCTG(#4), GAACGGGTG(#7), and GTGACGTAGG(#8). All primers produced several common bands among the strains. However, when primer #2 was applied, the electrophoregram showed the specific band at 1.8 kb region in Chungkyung-Chong. Hwangskoog-Chong, and KG101, and 1kb in the Jakyung-Chong 847913. In primer #4, 1.1kb band was shown in Chungkyung-Chong, Hwangskoog-Chong KG101, and Jakyung-Chong 79742. In primer #7, 700bp band was appeared in Jakyung-Chong 81783 and Jinjakyung of USSR. In primer #8,800bp band was observed only in Mimaki, comparing to another strains. When Similarity Index (SI) was calculated, Chungkyung-Chong and Hwangskoog-Chong, and Jakyung-Chong 81783 and Jinjakyung of USSR showed the most close SI, 0.11 and 0.08, respectively. The data of KG101, which showed the SI of 0.13 with the group of Chyngkyung-Chong and Hwangskoog-Chong,coincided with the fact that it was released from Hwangskoog-Chong by breeding process. The data of Jakyung strains indicated the significant variation among the strains. From these results, RAPD analysis method could be succesively applied to the classification and genetic analysis for breeding of Korean ginseng.
제초제 2, 4-D에 대한 고려인삼의 반응 2 : 2, 4-D의 경엽처리가 인삼엽의 광합성, 에칠렌가스 발생 및 지상부 생육에 미치는 영향
曺在星,元俊淵,辛崔順 韓國作物學會 1991 한국작물학회지 Vol.36 No.2
2, 4-D의 경엽처리가 인삼의 지상부 생육 및 광합성능력과 식물체의 Ethylene gas 발생등의 생리적특성에에 미치는 영향을 구명하기 위하여 본 실험을 수행하였던 바 그 결과를 요약하면 다음 과 같다. 1) 표준약량의 3배농도 2, 4-D 경엽처리시에도 엽장, 엽폭, 경장 및 경직경등 지상부 생육은 무처리와 전혀 유의차를 나타내지 않았으며 별다른 생육이상현상도 나타내지 않았다 2) 무처리구에 비해 2, 4-D의 경엽처리시 줄기에 대한 엽병의 각은 현저히 커졌으며 2, 4-D 처리농도가 증가될 수록 엽병각은 거의 직선적인 증가경향을 나타내었다 3) 2, 4-D의 경엽처리에 의해 인삼잎의 광합성능력은 현저히 저하되었으며 그 정도는 2, 4-D 처리농도를 증가함에 따라 현저히 크게 나타났다. 4) 2, 4-D의 경엽처리에 의한 인삼잎의 광합성저해현상은 표준 및 2배 농도 처리구에서는 처리후 3일에 정상으로 회복되었으나 3배 농도구에서는 처리후 12일 이후에 거의 회복단계가 되었다. 5) 표준 및 2배 농도의 2, 4-D 처리구에서는 무처리구와 같이 전혀 Ethylene gas의 발생을 측정할 수 없었으며 3배 농도 처리구에서만 미량의 Ethylene gas가 정량되었으나 이는 표준약량의 2, 4-D를 처리한 중에서 발생된 Ethylene gas 량의 1/20에 불과하였다. This study was conducted to define the effect of 2, 4-D foliar application on the plant growth. photosynthesis ability and ethylene gas production of the ginseng plant. Neither abnomal foliar changes occured nor any inhibition in the leaf and stem growth was resulted for the plants treated with 2,4-D concentrated three times of the recommended dosage. The angle of petiole to the stem became wider by 2, 4-D foliar application. The higher concentration treatment of 2,4-D made the petiole angle significantly wider. Foliar application of the herbicide 2,4-D inhibited photosynthesis of the ginseng leaf. The inhibition rate of photosynthesis was significantly increased with the application concentration of 2, 4-D. Inhibition in photosyn-thesis ability by 2,4-D application with doubled concentration was recovered in three days after treatment. When 2,4-D was treated with a concentration tripled the recommended dosage, 12 days were needed to recover the photosynthesis ability of the ginseng leaf. Ethylene gas was not detected from the ginseng plants treated with 2 times concentrated 2, 4-D. However, the ginseng plants produced 0.03-0.04 ppm ethylene gas when the application rate was increased 3 times. The amount of ethylene gas produced by ginseng plant treated with 3 times concentrated 2, 4-D was only 1/20 compared with the amount produced by the soybean plant treated with the recommended dosage of 2, 4-D.