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시험관내 공동배양법을 이용한 C형 간염 바이러스 Core 단백이 간섬유화 기전에 미치는 영향
신주엽 ( Sin Ju Yeob ),윤승규 ( Yun Seung Gyu ),왕진상 ( Wang Jin Sang ),허원희 ( Heo Won Hui ),류종순 ( Lyu Jong Sun ),배시현 ( Bae Si Hyeon ),최종영 ( Choe Jong Yeong ),양진모 ( Yang Jin Mo ),양세환 ( Yang Se Hwan ),성영철 ( Seo 대한소화기학회 2003 대한소화기학회지 Vol.42 No.5
The study of liver fibrogenesis by hepatitis C virus (HCV) has been limited due to the lack of an efficiency in vitro culture systems. In the present study, we investigated whether or not HCV core protein is directly related to liver fibrogenesis through stimulation of hepatic stellate cells (HSC). Methods: Human and rat HSC were isolated and we established an in vitro co-culture system of a stable HepG2-HCV core cell line which was transfected with HCV core gene and primary HSC. We performed immunocytochemical staining and Western and Northern blot analysis in the stimulated HSC by HCV ocre protein to identify the expression of transforming growth factor β1 (TGF-β1), transforming growth factor β receptor II (TGFβRII), α-smooth muscle actin (α-SMA) and connective tissue growth factor (CTGF). The expression of matrix metaloprotinase-2 (MMP-2) and collagen type I (Col I) in the culture media were measured by zymogram and ELISA, respectively. Results: The expression of TGF-β1 and CTGF was significantly higher in the stable HepG2-HCV core cell line than in HepG2 cells. Furthermore, the makers related to fibrosis such as α-SMA, TGF-β1, Col I, TGFRII and MMP-2 were highly experssed in the co-culture of stable HepG2-HCV core with HSC. Conclusions: HCV core protein may play a direct role in the fibrogenesis of chronic liver disease with HCV infection. (Korean J Gastroenterol 2003;42:400-408)
만성 간질환 환자에서 간섬유화의 정도와 혈청 히알루론산치를 이용한 간섬유화의 예측
김창욱 ( Kim Chang Ug ),윤승규 ( Yun Seung Gyu ),조병식 ( Jo Byeong Sig ),신주엽 ( Sin Ju Yeob ),장정원 ( Jang Jeong Won ),최종영 ( Choe Jong Yeong ),한남익 ( Han Nam Ig ),이창돈 ( Lee Chang Don ),정규원 ( Jeong Gyu Won ),선희식 ( 대한소화기학회 2003 대한소화기학회지 Vol.42 No.6
Backgrounds/Aims: The extent of hepatic fibrosis is important in chronic liver disease. Liver biopsy is essential for diagnosis of fibrosis. However, biopsy is invasive and may not represent the whole liver state. Serum hyaluronic acid (HA), a major component of connective tissues, was introduced as a useful non-invasive index of hepatic fibrosis. The aim of this study was to evaluate the relationship among HA, the degree of fibrosis, several hematologic and biochemical parameters in patients with chronic liver diseases or post state liver transplantation (PSLT). Methods: Total 102 cases were divided into 4 groups: 57 chronic hepatitis (CH), 12 cirrhosis, 21 hepatocellular carcinoma (HCC), 12 PSLT. HA was measured by enzyme-linked binding protein assay and evaluated in relation the degree of fibrosis, several hematologic and biochemical parameters. Results: Among four groups, HCC showed the highest HA and HA of HCC significantly higher than that of CH. The degree of fibrosis were correlated with HA. HA was correlated with age, platelet count and albumin but, not with ALT and PT. There is no significant relation between HA and the presence of acute rejection in liver transplantation. Conclusions: In chronic liver diseases, HA is a useful non-invasive index of hepatic fibrosis and disease severity. (Korean J Gastroenterol 2003;42:510-518)
사람 간세포의 분리 및 SV40 Large T antigen에 의한 세포의 불멸화
허원희 ( Heo Won Hui ),윤승규 ( Yun Seung Gyu ),신주엽 ( Sin Ju Yeob ),류종순 ( Lyu Jong Sun ),왕진상 ( Wang Jin Sang ),김창욱 ( Kim Chang Ug ),최종영 ( Choe Jong Yeong ),정규원 ( Jeong Gyu Won ),선희식 ( Seon Hui Sig ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 간세포는 간의 해독 작용이나 운반체 단백질의 합성 저장과 같은 중요 기능을 수행한다. 간세포를 이용한 연구에는 간의 재생(regeneration)과 분화(differentiation) 기작과 간염 바이러스(hepatitis virus) 관련 연구 등이 있다. 특히 간염 바이러스 연구는 형질전환 동물로만 이용해야 한다는 한계를 안고있기에 새로운 신약 백신이 개발되었더라도 신약에 대한 간염 바이러스의 치료 효과를 입증하기가 어려운 실정에 있다. 이