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- 저자 : 신연수 ( Yeun Soo Shin ) (검색결과 1 건)
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서울중앙병원에서 IMx를 이용한 혈장내 총Homocysteine의 측정
한태진 ( Tae Jin Han ),신연수 ( Yeun Soo Shin ),유승용 ( Seung Yong Yu ),장진연 ( Jin Yeon Jang ),조병철 ( Byoung Cheol Cho ),전사일 ( Sa Il Chun ),민원기 ( Won Ki Min ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Background : Elevated plasma homocysteine (HCY) levels are considered to be an independent risk factor in coronary 따tery disease, cerebrovascular disease, and peripheral vascular disease. Currently HPLC fluoremetric detection has been most widely used for the quantitation of homocysteine. Recently IMx homocysteine assay by use of a fluorescence polarization immlUloassay(FPIA) has been introduced that enables rapid quantitation of plasma homocysteine levels. We evaluated the performance of the IMx@ Homocysteine assay and compared it to routinely used HPLC method. Methods Within-run precision, between-run precision, degree of correlation, linearity, and reportable ranges were analyzed, according to National Committee for Clinical Laboratory Standarili잉 guidelines as much as possible. To establish a reference interval 148 apparently healthy adults were investigated (81 males, 67 females). For the methods comparison We selected 20 patients admitted to the Asan Medical Center. The total plasma homocysteine concentraton was measure by FPIA (lMx""J Homocysteine assay, Abbotte Laboratories, IL, USA) and by HPLC (HPll00, Hewlett Packard com., Germany) using Bio-rad@ Homocysteine kit (Bio-Rad Laboratories, CA, USA). The caIculations were performed with computer software, EP Suit(MarChem Associates, Concord, USA). Result : The within run coefficients of variations (CVs) of the low, medium, and of the high control were 1.8%, 1.5% and 1.5%. The between run CVs were 2.8%, 1.4% and 1.0%, respectively. Method was linear and passed lack of fit. Reportable range was found to be 0.5-50.0 μ molfL. The reference interval from hea1thy adu1ts was 10.3 i: 5.54(mean i: SD) μ molfL. Total plasma homocysteine levels according to sex were 11.9 i: 6. 89(mean i: SD) μ molfL for male, 8.39i: 2.03(meani: SD) μ mol/L for female, respectively. The comparison between FPIA (y) and HPLC(x) resu1ted in a linear regression analysis described by the equation : y=0.9463x-0.2364 with a correlation coefficient r=0.9961 Conclusions Linearity, pricision, reportable ranges of the IMx homocysteine were satisfactory. The IMx homocysteine also correlated well with HPLC method. The normal range, determined by testing apparent1y hea1thy adu1ts, is in agreement with the literature. πle IMx homocysteine assay is fast and easy to perform and very useful for routine determinations of plasma homocysteine levels.
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