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버드나무 (Salix Koreensis) 현탁배양 세포의 대사산물
박용구,이상구,신동일 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Cell suspension cultures for Salix koreensis was well established at the supplements of 2, 4-D with cytokinin particulary the combination of 1.0 ㎎/ℓ 2, 4-D with 0.1 ㎎/ℓ of zeatin. These combined rates of phytohormones are also effective to callus induction from S. koreensis leaf and its multiplication. Cultured media exhibited the great inhibitory effect on the germination of rice, barnyard grass and lettuce seeds, indicating the presence of biologically active substances in media. Several phenolic compounds such as pyrogallol, sinapic acid, cinnamic acid, tannic + gallic and p-chlorobenzoic acid were detected in the cell suspension culture. The inhibitory effect exhibited by cultured media may be partly attributed to these phenolic compounds.
은백양 (Populus alba) 엽육조직에서 원형질체 분리
손성호,박용구,우종호,설일환,신동일 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Factors affecting the isolation of protoplasts from mesophyll were investigated in P. alba. The yield of mesophyll protoplasts was obtained using leaves in vitro in 2.0% Cellulase R-10, 0.8% Macerozyme R-10, 1.2% Hemicellulase, 2.0% Driselase, 0.05% Pectolyase Y-23, and 0.6M mannitol in addition to DTT and MES buffer adjusted pH 5.6. Over 11.0×10^6 protoplasts per gram of leaf were produced using the condition. For protoplasts purification, the most favorable sucrose concentration of floating solution was 0.6M after washing them by CPW solution. This method of screening factors affecting protoplast isolation could be applicable to other species.