식물성 식품재료로부터 보체계 활성화 다당의 검색 및 그 활성검토

신광순,라경수,성하진,양한철,Shin, Kwang-Soon,Ra, Kyung-Soo,Sung, Ha-Chin,Yang, Han-Chul 한국식품과학회 1993 한국식품과학회지 Vol.25 No.3

식물성 식품재료를 대상으로 보체 용혈분석법$(TCH_{50})$ 을 이용하여 보체 활성화능(항보체 활성)에 대한 검색을 실시하였다. 총 38종의 식용 식물추출물 중 5종의 시료에서 대조구에 비해 60% 이상의 $TCH_{50}$ 감소를 일으키는 비교적 높은 활성을 나타냈으며 그 활성의 순서는 $1000{\mu}g/ml$ 시료 농도에서 생강>토란대>냉이>은행잎>달래이었다. 한편 가장 높은 활성을 보였던 생강에서 조제된 ZR-1의 경우 pronase 소화 후에는 활성의 변화가 없는 반면에 과요오드산 산화에 의해서는 급격한 활성의 감소를 나타냄으로써 ZR-1의 단백질 부위가 아닌 다당 부위가 활성에 기여함을 알 수 있었다. 또한 ZR-1의 항보체 활성은 $Ca^{2+}$ 이온 부재시 부분적인 감소현상을 보였으며, ZR-1을 정상인의 혈청과 반응 후 anti-humanC3를 이용하여 2차원 면역 전기영동을 행한 결과 C3의 분해산물을 관찰할 수 있었다. 또한 이들 획분은 $ACH_{50}$의 저해를 일으켰다. 동 결과로부터 ZR-1의 보체 활성화 양식은 classical pathway 뿐만 아니라 alternative pathway도 경유함을 알 수 있었다. Screenings were performed on edible plants to examine their complement-system activating ability (anti-complementary activity) by hemolytic complement assay $(TCH_{50})$. Among 38 kinds of plant extracts, 5 kinds showed relatively strong anti-complementary activity which decreased $TCH_{50}$ more than 60% comparison with control and the order of activity was Zingiber officinale>Colocasia antiquorum>Capsella bursapastoris>Ginkgo biloba>Alium monanthum in $1000{\mu}g/ml$. The anti-complementary activity of ZR-1 prepared from the root of Zingiber officinale which was showed the most potent activity, did not change by pronase treatment, but decreased greatly by periodate oxidation. These results indicate that not protein moiety but carbohydrate moiety in ZR-1 fraction may also contribute to the anti-complementary activity. Also, the anti-complementary activity of ZR-1 was reduced partially in the absence of the $Ca^{2+}$ ion. When crossed immunoelectrophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the ZR-1 in $Ca^{2+}$ free condition, a cleavage of C3 precipitin line was observed. Furthermore this polysaccharide fraction considerably inhibited $ACH_{50}$. These results also indicate that the mode of complement activation by polysaccharide from Zingiber officinale is via not only the classical pathway but also the alternative pathway.

B형간염(型肝炎) 표면항원(表面抗原)의 주면역원(主免疫原) 결정기(決定基)에 특이(特異)한 합성(合成) Peptide의 면역원성(免疫原性)에 관한 연구(硏究)

신광순,한수남,Shin, Kwang-soon,Han, Su-nam 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1

Many investigators have been pursuing various attempts so far to produce hepatitis B surface antigen(HBsAg) vaccines using the techniques such as isolation from plasma of chronic HBsAg carrier, recombinant DNA technique or preparation of synthetic peptides specific for immunogenic determinants. Hepatitis B virus can not grow on any cell lines by the tissue culture technique at the present time. The plasma of chronic HBsAg carrier is expensive and its source is limited. The HBsAg from the recombinant DNA technique gave still very low yield. Another approach, therefore, has been initiated to develop a synthetic hepatitis B virus vaccine. The possible use of several distinct synthetic vaccines in prophylaxis can be facilitated by availability of full synthetic immunogens. Peptides synthesized for potential application as antiviral vaccines have been mostly tested in the form of conjugates with carrier proteins, although the free synthetic peptide can be immunogenic. To understand basic knowledges on the antigenicity and immunogenicity of a synthetic peptide specific for major immunogenic determinant of HBsAg, a nonapeptide, $H_2N^{139}Cys-Thr-Lys-Pro-Thr-Asp-Gly-^{146}Asn-Aba$ COOH, which corresponds to HBsAg amino acid residues 139 to 147, was synthesized by the Merrifield's solid-phase method with a slight modification. The antigenicity and immunogenicity of this specific synthetic peptide were examined comparing with purified plasma-derived natural HBsAg. The results obtained are as follows; 1. The peptide synthesized showed the identical amino acid composition to the theoretical value. The degree of purification and molecular weight were acertained by methods of high performance liquid chromatography and mass spectrometry. 2. Using m-maleimidobenzoyl-N-hydroxysuccinimide ester as a conjugating agent, the synthetic peptide was conjugated to rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin. Their conjugation yields were 8.3, 9.5, 15.8, 13.5, and 11.2%, respectively. 3. The natural HBsAg was purified from plasma of chronic HBsAg carrier. By the electron microscopic observation of the purified natural HBsAg preparation, no Dane particles were observed and the preparation showed negative DNA polymerase activity. 4. Antigenicity of the synthetic peptide and the plasma-derived natural HBsAg was determined by competition radioimmunoassay using $^{125}I$-natural HBsAg. Their 50% inhibitions appeared as $90{\mu}g/ml$ and $0.12{\mu}g/ml$ for the synthetic peptide and the natural HBsAg, respectively. This indicates that the former was about 750-fold less antigenic than the latter. 5. Immunogenicity of the synthetic peptide was determined by administering the peptide-carrier conjugates into rabbits with and without Freund's complete adjuvant. Regardless the carrier proteins and adjuvant, positive immune responses to the synthetic peptide were observed. The higher antibody titers, however, were shown in the groups administered with Freund's complete adjuvant. 6. Immunizing dose 50% in mice of the various peptide-carrier conjugates was 5.47, 6.00, 65.16, 31.25 and $13.03{\mu}g/dose$ for rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin, respectively, while the natural HBsAg showed $0.65{\mu}g/dose$. 7. It was postulated that homologous proteins prefer to heterologous ones as the carriers.

경주마(競走馬) 혈청(血淸)의 단백질(蛋白質), Bilirubin, Sodium 및 Potassium 농도(濃度)와 Thymol Turbidity

신광순,나동진,문희주,조종후,Shin, Kwang Soon,Rha, Dong Jin,Moon, Hi Joo,Cho, Jong Hoo 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.1

Concentration of total protein, bilirubin, sodium and potassium in blood sera, and thymol turbidity of race horses, 78 males and 57 females aging from 2 to 5 years, were estimated. The results obtained were as follows: 1. Serum of race horses was low in protein concentrations as mean values of $5.48{\pm}0.77$ g/100ml. 2. Serum bilirubin was in normal range as mean values of total bilirubin, direct bilirubin and indirect bilirubin were $1.05{\pm}0.57$, $0.12{\pm}0.05$ and $0.93{\pm}0.58$ mg/100ml, respectively. 3. Mean value of serum thymol turbidity was $1.41{\pm}0.72$ S-H unit. 4. Mean values of serum sodium and potassium of race horses from Australia were $143{\pm}8.7$ and $3.85{\pm}0.53$ mEq/liter, respectively, and mean values of serum sodium and patassium of race horses from Japan were $142{\pm}9.4$ and $3.58{\pm}0.60$ mEq/liter, respectively.

Subacute Toxicity of Combined Vaccine (KGCC-95VI) Against Japanese Encephalitis and Hantaan Virus Infection in Rats

Kwang-Soon Shin(신광순),Hyo-In Yun(윤효인),Chul-Joong Kim(김철중),Kyou-Ho Oh(오규호),Dong-Ho Song(송동호),Jong-Il Park(박종일),Shin-Woo Cha(차신우),Chang-Nam Ahn(안창남),Hyeong-Soon Shin(신형순) 한국실험동물학회 1997 Laboratory Animal Research Vol.13 No.2

회원논단 : 한국식품위생안전성학회의 발자취

신광순 ( Kwang Soon Shin ) 한국식품위생안전성학회 2006 Safe Food Vol.1 No.1

미국 영화에 나타난 프랑스의 이미지 -사랑의 이미지를 중심으로

신광순 ( Kwang Soon Shin ) 성신여자대학교 인문과학연구소 2009 人文科學硏究 Vol.27 No.-

식물 기원의 면역활성 다당

신광순(Kwang-Soon Shin) 한국식품과학회 2012 식품과학과 산업 Vol.45 No.1

브랜드 아이덴티티 확립을 위한 국내 패션브랜드 플래그쉽 스토어 공간환경디자인 지침에 관한 연구

신광순(Shin, Kwang-Sun),최상헌(Choi, Sang-Hun) 한국실내디자인학회 2010 한국실내디자인학회 학술대회논문집 Vol.12 No.1

Regarding the awareness of brands, consumers" life style affects awareness of brands as well as consumption patterns according to rapid changes such as changes in overall mindset in society, development of various life cultures, and increase in income. By reflecting the social phenomena, brand identity makes consumers focus on their emotion or style, experience by themselves, prefer brands with good reaction from the people around them, and indeed purchase good brands again. The brand contains corporate cultures and values is currently built in a major business district for experiences and values of brands and product promotion through flagship stores. Therefore, this study aims to ensure brand identity through literary review and case analysis about flagship stores of fashion brands that are being currently built the most. In addition, it analyzes guidelines of space environmental design that Korean fashion brand flagship stores progress toward. The result shows that the space that can make mutual communication with consumers directly and indirectly is somewhat insufficient, so the plentiful expression of resting places and reception places is required. Moreover, detailed display as well as clothing that can read cultures and values of brands should be more expressed.

어린이집 유형과 보육교사 경력에 따른 직무만족도와 교사효능감의 관계

신광순(Shin, Kwang-Soon),유연옥(Yu, Younoak) 계명대학교 사회과학연구소 2016 한국사회과학연구 Vol.35 No.1

본 연구의 목적은 어린이집 유형과 보육교사 경력에 따른 직무만족도와 교사효능감의 관계를 알아보고자 하는 것이다. 연구대상은 U시에 위치한 가정 어린이집과 민간 어린이집에 근무하는 보육교사 284명이다(교직경력 0.5년~2년: 157, 5년~6년: 127). 보육교사의 직무만족도 설문지와 교사효능감 설문지를 활용하여 자료를 수집하고 이원변량분석과 Pearson의 적률상관분석을 적용하였다. 본 연구의 결과는 다음과 같다. 첫째, 직무자체, 상사, 승진, 직무만족도 전체에서 어린이집 유형과 경력 간의 상호작용효과가 나타났다. 가정 어린이집인 경우에는 경력이 낮은 교사(0.5-2년)가 경력이 높은 교사(5-6년)보다 만족도가 높았으나, 민간 어린이집인 경우에는 경력이 높은 교사가 낮은 교사보다 만족도가 높았다. 그리고 가정 어린이집 교사가 민간 어린이집 교사보다 상사와 동료에 대한 만족도가 높았고, 경력이 낮은 교사가 높은 교사보다 동료에 대한 만족도가 높게 나타났다. 둘째, 도전효능감은 민간 어린이집 교사가 가정 어린이집 교사보다, 경력이 높은 교사가 낮은 교사보다 높았다. 셋째, 가정 어린이집의 경력이 낮은 교사의 경우에 직무자체는 자기조절감, 도전감, 교사효능감 전체와 정적 상관이 있었고, 자기조절감은 동료, 직무만족도 전체와 정적 상관이 있었다. 이러한 연구결과는 보육교사의 직무만족도와 교사효능감을 높이기 위해 어린이집 유형과 교사 경력을 고려한 다각적인 방법이 필요함을 시사한다. The purpose of this study was to investigate the relationship between teacher efficacy and job satisfaction according to the type of child care center and career. The subjects of this study included a total of 284 teachers: 157 teachers with 0.5 to 2 years of teaching experience and 127 teachers with 5 to 6 years of teaching experience from home child care centers and private child care centers located in U city. Data were collected utilizing the child care teacher’s job satisfaction questionnaire and teacher efficacy questionnaire. For data analysis, ANOVA and Pearson"s product- moment correlation analysis were applied. The results of this study were as follows: First, results indicated interaction effects in the job itself, boss, job promotion, and overall job satisfaction. Teachers from home child care centers with less teaching experience had higher job satisfaction results than those with more teaching experience. In turn, private child care teachers with more teaching experience showed higher job satisfaction results than teachers with less teaching experience. Teachers from home child care centers showed higher scores in boss and coworker satisfaction than private child care center teachers. When looking at career satisfaction, teachers with less teaching experience showed higher job satisfaction than those with more experience. Secondly, teachers from private child care centers showed higher challenge efficacy scores than teachers from home child care centers. And teachers with more experience showed higher challenge efficacy scores than teachers with less experience. Thirdly, teachers from home child care centers with less experience indicated positive correlations between job itself and self-control efficacy, sense of challenge, overall teacher efficacy, and positive correlations between self-control efficacy and coworker, overall job satisfaction. These results of this study indicate the necessity of various methods concerning the type of child care center and teacher"s career to improve the working environment of child care centers for teachers.

단세포군항체를 이용한 한탄바이러스 검출용 ELISA법에 대한 연구

신광순(Kwang-Soon Shin) 大韓微生物學會 1992 大韓微生物學會誌 Vol.27 No.3