http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 신경오(Kyong Oh Shin) (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Sphingolipid Metabolites Profiling in Hair Growth
신경오 ( Kyong-oh Shin ) 한국피부장벽학회 2018 한국피부장벽학회지 Vol.20 No.2
Sphingolipids, a major component of hair lipids can determine the physicochemical properties of human hairs such as the chemical diffusion barrier, water retention and cell cohesion. In this study, we have developed a quantitation method for sphingolipids in animal and human hair. The lipid contents of sphingoid bases, sphingoid bases 1-phosphate, Ceramide (Cer) and dihydroceramide (DHCer) in hair were measured by liquid chromatography linked tandem mass spectrometry (LC-MS/MS). The established method was evaluated against quality parameters such as specificity, carryover, linearity and calibration, correlation of determination (R2), LOD, LOQ, precision, accuracy, and recovery. Specifically, analyzed DHCer levels were higher in normal hair roots to distal tip ends than Cer contents ranged from 1960.23 to 1373.24 pmol/g. Interestingly, DHCer were decreased significantly in alopecia patient upper hair fiber compared to alopecia patient side hair fiber. Specific ceramides (C16:0 and C24:1) in alopecia patient upper hair were observed in very low levels, while Cer was not change in alopecia hair. In experimental mouse model of alopecia hair and skin, Cer and DHCer were significantly low as well. These results suggested that hair DHCer content may be used as a potential biomarker for the progress in alopecia pathogenesis.
-
-
Derivatization reagents for Mass Spectrometry
신경오, 이부민, 이용문 충북대학교 약품자원개발연구소 2014 약학논문집 Vol.29 No.-
Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) has been frequently utilized for the sensitive and selective determination of the trace level compounds in biological samples. In LC/ESI-MS/MS, chemical derivatization is sometimes used to enhance the detection sensitivity of the analytes. The electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources of MS have contributed to the advancement of LC-MS and LC-MS/MS techniques for the analysis of biological samples. However, one major obstacle is the weak ionization of some analytes in the ESI and APCI techniques. In this review, we introduce high-sensitivity methods using several derivatization reagents for ionization enhancement. We also present an overview of chemical derivatization methods that have been applied to small molecules, such as amino acids and steroids, in biological samples.
RISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
