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- 저자 : 송홍규 ( Chang Joo Kwon (검색결과 2 건)
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Bunker C 유로 오염된 해사에서 bioremediation 에 의한 미생물군집의 변화
권창주,송홍규 ( Chang Joo Kwon,Hong Gyu Song ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2
The changes of microbial numbers and activities during the bioremediation of beachsand contaminated with recalcitrant Bunker C fuel oil were investigated using microcosms. The numbers of total hydrocarbon degrading bacteria were rapidly increased and reached to 107cells/g sand, which was 10^4-10^5 times of increase. The numbers of aromatic hydrocarbon degraders showed similar trends but 1/10 less numbers. The aerobic heterotrophs jumped from 10^6cells/g to 10^9cells/g during 2 weeks of incubation. FDA direct count which stained only metabolically active bacteria also showed similar level and changes. The FDA hydrolysis activity was doubled by bioaugmentation and dehydrogenase activity was increased at 5∼6 times than control samples. Although the microbial numbers and activities in contaminated beachsand were not coincided exactly with the removal pattern of Bunker C fuel oil, this study showed the positive effects of bioaugmentation of microbial consortium.
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Bioaugmentation 에 의한 해사에서의 Bunker C 유의 제거
권창주,송홍규 ( Chang Joo Kwon,Hong Gyu Song ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.1
A bacterial consortium composed of Pseudomonas sp., Acinetobacter sp. and Moraxella sp. showed the most highest degradability of Bunker C fuel oil with the inoculum size of 10^6cells/g sand and showed 37% of removal efficiency after 5 weeks of incubation, which is 3.7 times higher than uninoculated control sample. The maximum transformation rate was 100㎎ hydrocarbon/㎏ sand/day at 1∼2% of loading rates and temperatures of near 20℃, which was similar to biodegradability of crude oil in other studies. The addition of readily degradable auxiliary substrates such as glucose and powdered fish residue did not increase the biodegradation of Bunker C fuel oil due to the inhibition of hydrocarbon biodegradation by catabolite repression. Kerosene and diesel oil which were added to lower viscosity and induce cometabolism and synergism also could not increase the removal rates. Immobilized cells on vermiculite or sawdust resulted in lower degradability than untreated samples due to adsorption of hydrocarbons and decrease of contact between hydrocarbons and cells, and it is necessary to explore further to recover the degradation efficiency.
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