콩 유전자원의 지방함량과 지방산조성 평가 및 유용자원의 선발

송항림(Hang-Lin Song),장은규(Eun Kyu Jang),손은호(Eun Ho Son),이구환(Ku Hwan Lee),황세구(Se Gu Hwang),윤건식(Geon Sig Yun),이정동(Jeong-Dong Lee),김홍식(Hong Sig Kim) 한국육종학회 2015 한국육종학회지 Vol.47 No.3

This study was carried out to evaluate the oil contents and fatty acid compositions of 379 soybean germplasm accessions which is composed of 128 Korean, 214 Chinese and 37 USA accessions, and to select useful resources for the development of high oil content and quality fatty acid varieties in soybean. Korean and Chinese germplasm accessions were obtained from the National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, and USA accessions from the Plant Genetics and Breeding Laboratory of Kyungpook National University, Korea. The oil content ranges from 12.7∼26.0% with an average of 19.1% among accessions. The average contents and ranges of palmitic and stearic acid, saturated fatty acids (SFA), were 10.6% with a range of 4.1∼18.1% and 2.9% with a range of 1.9∼12.7% respectively, while those of oleic, linoleic and linolenic acid, unsaturated fatty acids (USFA), were 28.2% with a range of 15.3∼56%, 51.2% with a range of 24.4∼68% and 7.1% with a range of 3.4∼11.1% respectively. The average linoleic(ω-6)/linolenic(ω-3) acid ratio was 7.5 ranging from 3.8∼17, and the average SFA and USFA contents were 13.5% ranging from 6.7∼21.7% and 86.5% ranging 78.3∼93.4% respectively. Accessions with better essential properties were selected as useful resources for the development of high oil content and quality fatty acid varieties. Three accessions, Suinong 14, Dongnong 434 and Dongnong 46 from China contain oil higher than 25.0%, while five accessions including KLG 12073 and KLG 12074 from USA, Jilin 14 from China, Kwangan and Bongeui from Korea contain oleic acid higher than 50%. Eight accessions including KLG12083 and KLG12081 from USA contain linoleic acid higher than 60%. Seven accessions including KLG 12093 and KLG12096 from USA contain linolenic acid lower than 4%. Five accessions, KLG12074 and KLG12073 from USA, Kwangan and Bongeui from Korea, and Dongnong 47 from China were low linoleic acid(ω-6)/linolenic(ω-3) ratio resources with lower than 4.5.

국산 콩 장려품종의 Minerals, Oxalate 및 Phytate 함량

김기찬(Ki Chan Kim),황인국(In-Guk Hwang),윤건묵(Gun Mook Yoon),송항림(Hang Lin Song),김홍식(Hong Sig Kim),장금일(Keum Il Jang),정헌상(Heon Sang Jeong) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.7

본 연구는 국산 113종 콩 장려품종의 total oxalate(Ox), phytate(InsP?)와 calcium(Ca), magnesium(Mg), sodium(Na), zinc(Zn), potassium(K)을 분석하여 옥살산칼슘 결정 생성을 최소화할 수 있는 품종의 선발 및 안전한 콩 가공식품 제조를 위한 자료를 제시하고자 하였다. 113종의 콩 장려품종에서 Ca과 Mg 함량 분포는 각각 0.586~3.177과 0.559~3.085 ㎎/g이었으며, Ca는 다올콩은 3.177 ㎎/g으로 가장 높았고, Mg는 선흑콩은 3.085 ㎎/g으로 가장 높은 함량을 보였다. Ca과 Mg 사이에서 품종간 유의적인 차이가 나타나지 않았다. Ox와 InsP? 함량 분포는 각각 1.24(선흑콩)~3.81(다원콩)과 0.43(만리콩)~4.72(다기콩) ㎎/g 범위이었고, 옥살산칼슘 결정의 저해물질인 InsP?의 함량이 Ox의 함량보다 상대적으로 높은 함량 분포로 존재하였다. 또한 Ca, Mg, Ox 및 InsP? 함량 사이의 교차상관관계분석을 통해 Ca과 InsP? 함량이 Ox 함량보다 높은 선흑콩과 단미2가 옥살 산칼슘 생성의 잠재적 위험성을 최소화할 수 있는 품종으로 판단되었다. This study was conducted to select the soybean cultivar to minimize the formation of calcium oxalate, and investigate the ingredients for total oxalate (Ox), phytate (InsP?) and minerals such as calcium (Ca), magnesium (Mg), sodium (Na), zinc (Zn), and potassium (K) in 113 recommended soybean cultivars in Korea. Ca content ranged from 0.586 ㎎/g in Saealkong to 3.177 ㎎/g in Daolkong, and Mg content ranged from 0.559 ㎎/g in Taekwankong to 3.085 ㎎/g of dry seed in Seonheukkong. The total oxalate content ranged from 1.24 ㎎/g in Seonheukkong to 3.81 ㎎/g in Ilmikong, and InsP? content ranged from 0.43 ㎎/g in Mailikong to 4.72 ㎎/g of dry seed in Dagikong. In the cross-correlation analysis for the contents of Ca, Mg, Ox and InsP?, Seonheukkong and Danmi2 were selected to minimize the formation of calcium oxalate because the contents of Ca and InsP? were much higher than the content of Ox. These cultivars could be useful for producing soy foods beneficial to populations at risk for calcium oxalate kidney stones and for improved mineral bioavailability.

국산 장려콩으로 만든 두부의 Mineral, Oxalate 및 Phytate 함량과 품질특성

김기찬(Ki Chan Kim),황인국(In Guk Hwang),김현영(Hyun Young Kim),송항림(Hang Lin Song),김홍식(Hong Sig Kim),장금일(Keum Il Jang),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.7

국산 콩으로 제조한 두부의 품질특성과 원료 콩과 두부에서의 oxalate와 phytate의 함량을 분석한 결과는 다음과 같다. 두부의 수율 및 수분함량은 각각 137.77~201.91% 및 74.42~80.01% 범위로 나타났으며, 색도는 검은색 외피를 갖고 있는 청자콩, 다원콩, 선흑콩 및 검정콩4호가 L값은 53.05~63.23, a값은 3.18~5.85 및 b값은 3.28~6.02로 다른 품종들에 비해 L값은 감소하였고 a값은 증가하였다. 두부의 hardness, cohesiveness, elasticity는 품종에 따라 각각 537.36~1696.05 g, 0.20~0.37 및 2.27~3.76으로 차이를 보였다. 두부의 calcium 함량은 0.586~1.488 ㎎/g 범위였으며, oxalate 함량은 0.01~0.40 mg/g 범위에서 검정콩4호로 조제된 두부가 가장 높은 함량을 나타내었고 phytate는 0.01~0.41 ㎎/g으로 다원콩으로 만든 두부가 가장 높은 함량을 나타내었다. 콩에서 두부로의 oxalate 및 phytate의 전이율은 각각 0.31~19.70 및 0.41~19.70%로 나타났다. 따라서 국산 장려콩을 이용하여 두부를 제조할 경우 phytate의 전이율이 높고 oxalate의 전이율이 낮은 한남콩, 다원콩 및 보광콩이 좋을 것으로 생각된다. This study was conducted to investigate the changes of mineral, oxalate and phytate during tofu processing with Korean recommended soybean cultivars. Fourteen cultivars were selected by distribution of calcium, oxalate and phytate contents. Tofu was manufactured and analyzed for quality characteristics, mineral, oxalate and phytate contents. The yield and moisture contents of tofu ranged from 137.77% in Anpyeongkong to 201.91% of Geomjeongkong 4, and 74.42 (Bongeuikong)~80.01% (Hojangkong), respectively. The lightness (L-value), redness (a-value) and yellowness (b-value) ranged from 53.05 (Dawonkong) to 86.16 (Jipumkong 2), -2.04~5.85, and from 3.28 in Geomjeongkong 4 to 16.17 of Cheongjakong, respectively. The hardness of tofu with selected soybean cultivar ranged from 537.36 g (Dongpuktae) to 1696.05 g (Jinpumkong 2). The highest calcium content was 1.488 ㎎/g in Dawonkong, oxalate and phytate in tofu were high at 0.40 ㎎/g in Geomjeongkong 4 and 0.41 ㎎/g in Dawonkong, respectively. The transfer ratios of oxalate and phytate from soybean to tofu were ranged from 0.31~19.70 and 0.41~19.70%, respectively.

국산 장려콩으로 만든 두유의 Mineral, Oxalate 및 Phytate 함량과 품질특성

김기찬(Ki Chan Kim),황인국(In Guk Hwang),김현영(Hyun Young Kim),송항림(Hang Lin Song),김홍식(Hong Sig Kim),장금일(Keum Il Jang),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.8

국산 콩으로 제조한 두유의 품질특성과 무기성분, oxalate 및 phytate 함량을 분석한 결과는 다음과 같다. 두유의 수율 및 고형분 함량은 각각 8.43(보광콩)~9.15 mL/g(봉의콩과 한남콩) 및 4.37°Brix(안평콩)~7.17°Brix(봉의콩) 범위로 나타났으며, pH와 총산도는 각각 6.43~6.86 및 1.48~1.65% 범위를 나타내었다. 점도는 15.73(다원콩)~20.80 cP(한남콩) 범위를 나타내었다. 색도는 검은색 외피를 갖고 있는 청자콩, 다원콩, 선흑콩 및 검정콩4호로 만든 두유의 L값이 작았고 a값은 크게 나타났다. Calcium 함량은 0.714(금강콩)~1.589 ㎎/g(선흑콩) 범위였으며, oxalate 함량은 0.24(안평콩)~2.14 ㎎/g(한남콩), phytate 함량은 0.63(호장콩)~ 2.18 ㎎/g(안평콩) 범위였다. Calcium/oxalate 함량 분포에서 oxalate 함량이 calcium보다 넓은 분포를 나타내었으며, phytate/ oxalate 간에는 phytate가 넓은 분포를 나타내었다. 콩에서 두유로의 oxalate 전이율은 13.6(안평콩)~77.6%(진품콩2호) 범위였으며, phytate는 13.9~87.5%로 동북태가 가장 높았고 호장콩이 가장 낮았다. 이상의 결과를 종합해보면 국산 장려 콩을 이용하여 두유를 제조할 경우 phytate의 전이율이 높고 oxalate의 전이율이 낮은 봉의, 안평콩 및 보광콩이 좋을 것으로 판단되었다. This study was conducted to investigate the changes of quality characteristics, and mineral, oxalate and phytate content during soymilk process. The yields of soymilk ranged from 8.43 mL/g in Bokwangkong to 9.15 mL/g in Bongeuikong and Hannamkong, and total soluble solid contents were ranged from 4.37°Brix in Anpyeongkong to 7.17°Brix in Bongeuikong, respectively. The pH and total acidity of soymilk ranged from 6.43 to 6.86 and from 1.48% to 1.65%, respectively. The viscosity of soymilk was the highest value of 20.80 cP in Hannamkong and the lowest value of 15.73 cP in Dawonkong. The highest value of calcium content of soymilk was 1.589 ㎎/g in Seonheukkong, and oxalate and phytate in soymilk were high at 2.14 ㎎/g in Hannamkong and 2.18 ㎎/g in Anpyeongkong, respectively. The transfer ratio of oxalate from soybean to soymilk was the highest value of 77.6% in Jinpumkong 2, and one of the phytate was the highest value of 87.5% in Dongpuktae and the lowest value of 13.9% in Hojangkong.

콩 유전자원의 종실 저장단백질 변이 및 유용자원 발굴

허건 ( Gun Hur ),장성진 ( Seong Jin Jang ),이윤호 ( Yun Ho Lee ),송항림 ( Hang Lin Song ),김성진 ( Seong Jin Kim ),정헌상 ( Heon Sang Jeong ),조용구 ( Yong Gu Cho ),김홍식 ( Hong Sig Kim ) 한국콩연구회 2008 韓國콩硏究會誌 Vol.25 No.1

한국야생콩 200종, 한국재래콩 300종 및 도입유전자원 398종을 포함한 총 898종에 대하여 SDS-PAGE에 의한 종실 단백질의 변이를 구명하고 변이자원을 발굴하여 고품질 콩 품종개발의 기초자료로 이용코자 수행하였다. 1. 7S함량은 평균 45.85%로서 7.16-66.24%의 범위였고, 변이자원은 α` subunit 결실 6종, α subunit 결실 1종, α` 및 α subunit 동시 결실 2종, β subunit 결실 6종, α 및 β subunit 동시저하 1종의 총 16종이 발굴되었다. 2. 11S함량은 평균 54.16%로서 33.76-89.67%의 범위였고, 변이자원은 A3 subunit 결실 6종, Acidic 저하 3종, Basic 저하 5종, Acidic 및 Basic 동시저하 1종의 총 15종이 발굴되었다. 3. 11S/7S 비율은 평균 1.18로서 0.5-8.68의 범위였으며, 도입유전자원의 변이가 가장 컸고, 다음으로 한국 야생콩이 컸으며 한국 재래콩이 작았다. 11S/7S 비율이 높은 자원으로는 CS1733, CS79, CS1228, CS1230 및 CS667의 5종이 발굴되었다. 4. Lipoxygenase 결실 변이자원은 11종, Kunitz trypsin inhibitor 결실 변이자원은 12종이 발굴되었다. To identify soybean genetic resources with specific seed proteins, a total of 898 soybean resources including 200 Korean wild soybeans, 300 korean local soybeans and 398 introduced soybeans were screened to evaluate variations of seed storage protein by SDS-PAGE. 7S concentrations of seed storage protein ranged from 7.16% to 66.24% with a mean of 45.85%. Sixteen variants including six lacking of α` subunit, one lacking of α subunit, two lacking of both α` and α subunits, six lacking of β subunit and one low level of both α and β subunits were detected for 7S seed storage protein. 11S concentrations of seed storage protein ranged from 33.76% to 89.67% with a mean of 54.16%. Fifteen variants including six lacking of A3 subunit, three low level of acidic group, five low level of basic group were detected for 11S seed storage protein. 11S/7S ratio of seed storage protein ranged from 0.5 to 8.68 with a mean of 1.18. Variation of 11S/7S ratio was largest in introduced soybeans, medium in Korean wild soybeans, and smallest in Korean local soybeans among three soybean groups. Of 898 soybean germplasms, CS1733, CS79, CS1228, CS1230 and CS667 were extremely high in 11S/7S ratio of seed storage protein. Eleven and twelve variants were detected for lacking lipoxygenase and for lacking kunitz trypsin inhibitor, respectively.