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      • 實驗家兎의 大腿骨頸部 骨折에 있어서 筋肉莖骨移植術의 實驗的 硏究 및 臨床治驗例

        宋重根,邊永壽 고려대학교 의과대학 1977 고려대 의대 잡지 Vol.14 No.1

        We made a planned study on ten rabbits for the purpose of reducing and preventing the complications and sequales such as the delayed union and the aseptic necrosis of the head in the treatment of the femoral neck fractures. The midneck of the femur of each rabbit was experimentally fractured and was immobilized by the insertion of two K-wires into the fractured fragment. Then we made an Muscle Pedicle Bone Graft on the area extending from the greater trochanter to the head of the femur inserting the muscle pedicle bony fragment, Rectus femoris and Sartoris originated from middistance between Anterior Superior Iliac Spine and Anterior Inferior Iliac Spine were also detached with the underneath bone. The roentgenographic and microscopic findings at 6th and 8th weeks after operation, the results were so satisfactory that we applied on it clinically. The summary of the results were as follows. 1. Group of experimental rabbits. 1. Their was roentgenographic evidence that the fracture gap had disappeared and the density of the grafts was never changed with the comparison to the other femoral head and neck. 2. Histological study, fracture line reveals complete bone union except the foci of ingrowing of chondrous tissue and fibous tissue. 3. Clinical application. 1) There was roentgenographic evidence that complete bone union at the fracture site and no evidence of aseptic necrosis of femoral head. 2) The patient was carrying a relatively normal activity without the limited range of motion of the hip joint and other complications.

      • 局所貧血로 因한 筋肉變化에 關한 臨床的 硏究

        宋重根,李弘鍵 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.1

        The purpose of this study is to elaborate the early ischemic changes in human skeletal muscle using HBFP(hematoxylin basic fuchsin picric acid) stain. Author carried out this clinical study with twenty operated cases and an experiment with ten mature rabbits to detect early degeneration of skeletal muscles. Twenty clinical cases were devided into two groups : ten fresh trauma cases, and ten old trauma or nontrauma cases. Pneumatic tourniquet was applied with 300 ㎜Hg pressure on the upper extremity and 550 ㎜Hg pressure on the lower extremity. Muscle biopsies were obtained from belly of brachioradialis in the upper extremity and tibialis anterior muscle in the lower extremity, respectively. Ten mature rabbits, weighing 2,000-2,500gm, were biopsied from the belly of tibialis anterior muscle at 5 minutes, 30 minutes, 60 minutes, 90 minutes, and 120 minutes after application of a devised the same tourniquet with 200 ㎜Hg pressure. After release of the tourniquet, also biopsied a piece of skeletal muscle on the first, second, third and sixth day, respectively. All specimens of skeletal muscles were stained with HBFP method and hematoxylin and eosin method, respectively. The results obtained are as follows: 1. In ten fresh trauma cases, mean value of fuchsin positive areas were 87.5% at 5 minute, 89% at 30 minutes, 91.5% at 60 minutes, 93% at 90 minutes and at 120 minutes after the tourniquet application. 2. In ten old trauma or nontrauma cases, mean value of fuchsin positive areas were 25% at 5 minutes, 43.5% at 30 minutes, 65% minutes, 80.5% at 90 minutes and 89% 120 minutes after the tourniquet application 3. In experimental group of mature rabbits, mean value of fuchsin positive areas were 16 % at 5 minutes, 31% at 30 minutes, 50.5% at 60 minutes, at 90 minutes and 76.5% at 120 minutes after the tourniquet application. And HBFP stain also disclosed mean value of fuchsin positive areas 43% on the first day, 35% on the second day, 23.5% on the third day and third day and 7% on the sixth day after the tourniquet release. The above results suggest that HBFP staining method is useful to detect early degenerative change and to diagnose the diagnose the progress of degeneration of skeletal muscles in clinical cases.

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