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고초균에서 발현된 벼 키틴분해효소 ( CH16 ) 의 병원성 곰팡이 생장 저해 효과
손종문(Jong Mun Son),판철호(Cheol Ho Pan),소민영(Min Young So),채영암(Young Am Chae),김수일(Su Il Kim) 한국키틴키토산학회 2001 한국키틴키토산학회지 Vol.6 No.1
N/A To investigate whether or not translational product of rice chitinase cDNA CH16 has antifungal activity, recombinant CH16 was produced in microorganism and its antifungal activity was tested in vitro. The rice chitinase cDNA, CH16, which encodes a basic class I chitinase, was expressed in Bacillus subtilis. To prepare the expression plasmid, CH16 was inserted into the multicloning site of a plasmid, p8A-1, which contains the promoter and signal sequence of Bacillus amylase. The expression cassette consisting of promoter, signal sequence, and ORF of CH16 was subcloned into the shuttle vector, pUBL140, which can be replicated in E. coli as well as B. subtilis. The resulting plasmid was named as pUBLCH16. Expression plasmid was transformed in B. subtilis WB600 deficient in six extracellular proteases and transformant expressed recombinant chitinase, CH16, at the stationary phase. CH16 protein showed the growth inhibition of Fusarium oysporum R2 which was isolated from the root of Rhemannia glutinosa and specifically infected to Rehmannia root.