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α-Fetoprotein의 측정(測定) 방법별(方法別) 비교(比較) 검토(檢討)
성호연 ( Ho Yun Sung ),박관순 ( Kwan Soon Park ) 대한임상검사과학회 1982 대한임상검사과학회지(KJCLS) Vol.14 No.1
It is an introductory study of AFP measurement of normal adults sera and AFP positive sera, measured by EIA, RPHA, and DID methods. 1. The results obtained by the quantitative method between EIA and RPHA were analogous, but DID methods howed low sensitivity. 2. In the case of the concentration of AFP over 10,000ng/ml, we had to dilute the specimen times to obtain the result within the measuring field. 3. The results of AFP were appeare1 all under 10mg/m with EIA method from 30 normal adult`s sera. 4. EIA method needed a sophisticated technique because of it`s complexity and length of time
Dye-Binding법(法)에 의한 뇌척수액단백정량(腦脊髓液蛋白定量)에 관(關)한 연구(硏究)
이국성 ( Kook Sung Lee ),성호연 ( Ho Yun Sung ) 대한임상검사과학회 1984 대한임상검사과학회지(KJCLS) Vol.16 No.1
A Literature review and comparative study about quantitative analysis of protein in Cerebrospinal Fluid by the Trichloroacetic acid (T.C.A) method and Dye-Binding method using Coomassie Brilliant Blue G-250 (C.B.B) were studied. The results were as folows; 1. The absorbance of standard calibration pattern for various concentration of protein using the method of C.B.B Dye-Binding shows continually decreasing as more dye is bound to protein, but the pattern shows stable and regular curvature. 2. At the concentration lower than 100mg/dl of protein , the Dye-Binding method is more sensitive than the T.C.A method , but T.C.A method is still useful since there is a linearity for the higher concentration of protein. 3. The Dye- Binding method is a rapid and microquantitative method, which requires only 100μl of serum and 10 minutes of time at room temperature. lt is more valuable for the determination of higher concentration of protein needed for dilution. 4. A Comparison of T.C.A method and Dye-Binding method shows good correlation coefficience and linear regression equation. 5. ln the precision test, the both method shows satisfied result of coefficient of variation of 1.0~1.66% in T.C.A method, and of 1.48~2.71% in Dye-Binding method. 6. The color developed by the dye was very stable within 4 hours.
원저(原著) : 친자확인 검사에서 발견된 cis-AB가족 1예
최범열 ( Bum Ryoul Choi ),이동범 ( Dong Beom Lee ),최중문 ( Joong Moon Choi ),성호연 ( Ho Yun Sung ) 대한임상병리사협회 1996 임상수혈검사학회 발표자료집 Vol.3 No.1
저자들은 일상 혈액형 검사에서 혈구형 검사만을 실시할 경우 발견할 수 없었던 cis-A2B3형 아버지와의 A1형 어머니 사이에서 O형의 아들이 출생된 cis-AB형 가계를 경험하였기에 문헌고찰과 함께 보고하며 수혈검사시 ABO식 혈액형에서 필히 혈구형과 혈청형을 실시하여야 할 것임을 제안하고자 한다. ABO discrepancy is difficult to inspections of one`s child confirmation and transfusion. Cause of ABO discrepancy have several of things and the authers introduced 1 case of the causes to you. ABO blood group is determinated by A and B genes located on each chromosomes which are inherited from parents.But cis-AB has been reported unusual inheritive of A and B genes on the same chromosome. By a routine blood typing, we have found that parent of cis-A2B3 type and A1 type can bear children of O type blood. From the fact above-mentioned, we have concluded that the above case cannot be predicted from Mendel`s laws. Therefore, We intend to report the truth with the conference books and propose that medical technologist must be tested cell and back typing certainly pretransfusion test.