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미세먼지 자극으로부터 지모추출물, 베타글루칸, 폴리감마글루탐산 혼합물의 안티폴루션 효능 평가
서수경 ( Soo-kyung Seo ),구창섭 ( Chang-sub Ku ),손보미 ( Bomi Son ),이수경 ( Su-gyeong Lee ),강은미 ( Eun Mee Kang ),김미옥 ( Miok Kim ),홍정현 ( Jung Hyun Hong ),김선화 ( Seonhwa Kim ),신문삼 ( Moon Sam Shin ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.4
This study characterized Prostaglandin E2 (PGE2) production from Raw264.7 cells stimulated with particulate matter (PM1648a) and tested anti-pollution capacity of mixtures of Anemarrhena asphodeloides root extract, β-glucan and poly- γ-glutamic acid (the complex). In order to assess cytotoxicity, we treated the Raw264.7 cells with the complex using the lactase dehydrogenase (LDH) assay method. To measure cell viability, we treated HaCaT cells with the complex using the MTT assay method. To test scavenging activity, the DPPH assay method was used. To test the anti-oxidant effect of the complex, we treated HaCaT cells with the pro-inflammatory mediator particulate matter (PM1648a) and with PM1648a and the complex, then measured the concentration of reactive oxygen species (ROS) within the cells using the dichlorofluorescin diacetate (DCF-DA) assay method. The complex had cytotoxicity below 6.5% and cell viability above 91.3% at a concentration of 0.05%. Also, the complex showed scavenging activity above 50% at a concentration of 1.0%. In addition, ROS production at the 0.05% complex concentration was reduced to 61.0%. In a clinical experiment, the subjects who had skin urtication used a cosmetic product containing mixtures of 1.0% Anemarrhena asphodeloides root extract, 0.4% β-glucan and 0.5% poly-γ-glutamic acid for 4 weeks. The experimental group showed a statistically significant improvement in skin humidity, desquamation index and skin urtication. Based on the results with in vitro test and clinical experiment, mixtures of Anemarrhena asphodeloides root extract, β-glucan and poly-γ-glutamic acid could be used as cosmetic anti-pollution materials.