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방화곤충에 의한 유전자변형 작물 화분 이동성 조사를 위한 프로토콜 개발
백형진 ( Hyeong Jin Baek ),손수인 ( Soo In Sohn ),조명래 ( Myoung Rae Cho ),이관석 ( Gwan Seok Lee ),오영주 ( Young Ju Oh ),박종석 ( Jong Sug Park ),이기종 ( Ki Jong Lee ),오성덕 ( Sung Dug Oh ),서석철 ( Seok Choel Suh ),류태훈 ( 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
The aim of this study was to develop a protocol for evaluation of pollinator insect-mediated gene transfer from GM crop to non-GM crop. Insect fauna were investigated in three plots planted with herbicide-resistant transgenic soybean(RRS), G.max L.cv. Taekwang and G. soja. The occurrence number of bee in plot of G. soja and the rate of pollen attachment on the body parts of bee were analyzed at different times. Pollens were collected from the legs of bee trapped in the GM soybean plot. Using the pollen genomic DNA, PCR was performed with GM- and lectin- specific primers to establish the analyzing condition. There was an insignificant difference in the number if insect between the GM soybean plot and non-GM soybean plot. During the flowering time, the rate of bee occurrence was highest at the noon. Since the lectin gene was amplified from the pollen DNA, we confirmed that the harvested pollen was originated from the soybean.
연구보문 : 환경생물 ; 형질전환 콩 재배가 근권 토양 미생물상에 미치는 영향
이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),이장용 ( Jang Yong Lee ),이부영 ( Bu Young Yi ),오성덕 ( Sung Dug Oh ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈 ( Tae Hun Ryu ),김경환 ( Kyung Hwan Kim ),박종석 ( Jo 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4
BACKGROUND: Soybean [Glycine max (L.) Merrill] is legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn`t show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified γ-tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),권용정 ( Yong Jung Kwon ),김진서 ( Jin Seo Kim ),이장용 ( Jang Yong Lee ),박범석 ( Beom Seok Park ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈· 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2
해충저항성 Bacillus thuringiensis (Bt) 벼의 비표적곤충인 벼물바구미(Lissorhoptrus oryzophilus)에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 72시간-LC50은 0.245 ml/L(95% 신뢰한계는 0.195~0.307 ml/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간- LC50은 0.257 ml/L(95% 신뢰한계는 0.199~0.331 ml/L)이었다. 72시간-LC50은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-LC50이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 LC50값에 유의성이 없는 것으로 판단된다. Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis, As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-LC50 values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively, As the 72h-LC50 vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-LC50 values were not significant, Thus, we found out there was a strong possibility that Bt rice didn`t affect the insecticide susceptibilities of nontarget insects.
연구보문 : 환경생물 ; 분자생물학적 분석을 통한 Bt 배추의 토양미생물상 영향 비교평가
손수인 ( Soo In Sohn ),오영주 ( Young Ju Oh ),오성덕 ( Sung Dug Oh ),김민경 ( Min Kyung Kim ),류태훈 ( Tae Hoon Ryu ),이기종 ( Ki Jong Lee ),서석철 ( Seok Choel Suh ),백형진 ( Hyeong Jin Baek ),박종석 ( Jong Sug Park ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.3
본 연구의 목적은 Bt 배추의 토양미생물 군집에 미치는 영향을 조사하기 위한 것이다. 토양미생물 조사에 앞서 토양화학성분을 분석한 결과, Bt 배추와 일반품종 배추 근권토양 간 화학성분의 유의성 있는 차이는 없는 것으로 조사되었다. 생육 최대 성숙기의 토양미생물 군집밀도를 조사했을 때 Bt 배추 근권토양의 미생물 군집밀도 범위가 일반 배추 근권토양의 미생물 군집밀도 범위내에 속하는 것으로 나타났다. 월별 DGGE 분석결과 일반품종 배추 근권토양 미생물 군집에 비해 Bt 배추 근권토양 미생물 군집의 변이는 없었다. 형질전환 벡터 유래 유전자부위를 이용하여 유전자의 수평이동성을 조사했을 때 Bt 근권토양 유래 DNA에서는 벡터 유래 유전자가 검출되지 않았다. The aim of this study was to investigate the possible impact of Bt Chinese cabbage on the soil microbial community. Microbial communities were isolated from the rhizosphere of one Bt Chinese cabbage variety and four varieties of conventional ones and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional Chinese cabbages were observed to have an insignificant difference. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures were very similar to each other and this genetic stability of microbial communities was maintained throughout the culture periods. Analysis of dominant isolates in the rhizosphere of transgenic and conventional Chinese cabbages showed that the dominant isolates from the soil of transgenic Chinese cabbage belonged to the Bacilli and Alphaproteobacteria, while the dominant isolates from the soil of conventional cabbage belonged to the Holophagae and Planctomycetacia, respectively. These results indicate that the Bt transgenic cabbage has no significant impact on the soil microbial communities.