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RISS 인기검색어
- 검색키워드
- 저자 : 서교성 (검색결과 1 건)
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This thesis describes a method to improve a biosystem ability using micro encapsulation. In chapter 1, rapid measurement of biochemical oxygen demand by a flowing injection analysis method using Saccharomyces cerevisiae cells encapsulated in a high cell density. In chapter 2, the method for utilizing micro encapsulation was suggested by using cosmeceutical activity of Kalopanax septemlobus root extract. [Chapter 1] The most widely used measurement of organic matter applicable to both wastewater and polluted waters is 5 day biochemical oxygen demand or BOD5. this determination involves measurement of dissolved oxygen used by microorganism in biochemical oxidation of organic matter. The biochemical oxygen demand (BOD) determination was studied using a novel flow injection analysis (FIA) system with encapsulated Saccharomyces cerevisiae cells and an oxygen electrode and was compared with conventional 5-day BOD tests. S. cerevisiae cells were packed in a calcium alginate capsule at a dry cell weight of 250 g/l of capsule core. The level of dissolved oxygen (DO) was reduced due to the enhanced respiratory activity of the microbial cells when the injected nutrient passed through the bioreactor. The decrease in DO (?DO) was intensified with the amount of microbial cells packed in the bioreactor. However, the specific ?DO decreased as the amount of cells loaded in the bioreactor increased. The ?DO value was dependent on the pH and temperature of the mobile phase and reached its maximum value at 35?C and pH 7?8. Also, ?DO became larger at longer response times as the flow rate of the mobile phase decreased. The measurement of ?DO was repeated more than six times consecutively using a 20-ppm standard glucose and glutamic acid solution, which confirmed the reproducibility with a standard deviation of 0.95%. A strong linear correlation between ?DO and BOD was also observed. The 5-day BOD values of actual water and wastewater samples were in accordance with the BOD values obtained by this FIA method using encapsulated S. cerevisiae cells. Unlike the cell-immobilized bead system, there was no contamination of the bioreactor resulting from any leak of yeast cells from the sensor capsules during BOD measurements. [Chapter 2] This study was investigated for the cosmeceutical activity of Kalopanax septemlobus root extract and capsulation containing active ingredients for the application of cosmetic, functional food and health care industries. The following conclusions were obtained. Polyphenol contents, which have a various activity such as prevent cavities, hypertension inhibition, anti-oxident and anti-cancer, showed 232.4 mg/l in the Kalopanax septemlobus root extract and especially, Ethyl acetate fraction was higher than that of the others. Anti-oxidant activity experiments in the this study were investigated by using the electron donating activity(EDA), ABT radical cation decolorization, superoixde radical inhibition activity, hydrogen peroxide inhibition activity, SOD-like activity. Anti-oxidant activity experiments, except for SOD-like activity were found in the anti-oxidant activity. In particular, ethyl acetate fraction was able to check the most powerful anti-oxidants. Anti-inflammatory activity of Kalopanax septemlobus root extract was determined by measuring the nitrite activity, anti-wrinkle activity was confirmed by the measurement of collagenase inhibition activity. The experimental results confirm the effect of both valid and in particular, ethyl acetate fraction was able to check the most powerful effect. but, astringent and tyrosinase inhibition activity did not show the effect. Kalopanax septemlobus root extract to determine the cytotoxicity was determined by MTT assay, 100ug/ml had little or no effect. The results of structural analysis were detected Kalosaponin A in the ethyl acetate fraction and Kalosaponin B in the butanol fraction by NMR PMMA capule containing Kalopanax septemlobus root extract was confirmed by SEM. The average particle size by Particle size analysis was found to be about 7.4?m. Releasing test of capsule was measured three kinds of solution (ethanol solution, ethanol/water (7:3, V/V), water) according to the polarity. All solutions up to 1 hour showed a quick release phenomenon since its release rate is delayed by 7 hours. Micro encapsulation containing natural activity ingredients for application of industries.
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