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유치도뇨관 크기 감소 및 정보제공이 수술 후 유치도뇨관으로 인한 불편감과 간호요구도에 미치는 효과: 회복실에 입실한 성인남자 환자를 중심으로
백현주,이혜미,Baek, Hyun Ju,Lee, Hye Mi 한국가정간호학회 2014 가정간호학회지 Vol.21 No.2
Purpose: This study was carried out to investigate the effect of a decrease in indwelling catheter size and preoperative education on bladder discomfort and nursing needs. The study was conducted on patients in the recovery room after their surgery. Methods: This study is a randomized control trial. Data were collected from September 2011 to February 2012 at a university hospital in Bundang, Korea. The sample consisted of 75 patients, excluding one patient from the experimental group 2. From among the patients that were sent to the ICU, 24 were in experimental group 1, 24 were in experimental group 2, and 27 were in the control group. Data were collected and analyzed using a chi-square test, t-test, and one-way ANOVA. Results: There were significant differences between the bladder discomfort experienced by patients in experimental group 1 and the control group, and experimental group 2 and the control group. Then nursing needs of the control group were higher than those of the experimental groups 1 and 2. Conclusion: The decrease in indwelling catheter size and preoperative nursing education is an effective nursing intervention in perioperative care.
수변 낙엽퇴적층에서 분리한 호열성 Geobacillus의 물질 분해 특성
백현주,조영근,안태석,Baek, Hyun-Ju,Zo, Young-Gun,Ahn, Tae-Seok 한국미생물학회 2009 미생물학회지 Vol.45 No.1
수변 낙엽퇴적층에서 호열성 세균의 분포와 물질분해 양상을 파악하기 위하여, 호열성 세균을 분리하고 그 특성을 조사하였다. 고분자 유기물의 분해력이 높고 $60^{\circ}C$에서 생장하는 균주들을 탐색한 결과, 10개의 균주가 선별되었다. 16S rRNA 유전자 염기서열을 결정하고 계통분석한 결과, 모두 Geobacillus 속으로 동정되었다. 균주들의 최적 성장온도는 $55^{\circ}C$와 $60^{\circ}C$, 최적 pH는 초기 pH가 6.0${\sim}$8.0일 때로 나타났으며, 염분 3%일 때를 기준으로 성장속도의 저해가 뚜렷하게 나타났다. 균주의 효소 활성도와 API 50 CHB kit에 의한 탄수화물 이용도를 바탕으로 균주의 생태학적 기능을 알아본 결과, G. stearothermophilus 균주들과 G. kaustophilus 균주들의 특성은 다른 종의 균주들과 명확히 구분되었으며, 나머지 균주들은 최적 성장환경의 생태학적 특징이 다양하게 나타나, 종별 구분과 정확히 일치하지는 않았다. 약산성 환경에서 최적 성장하는 G. stearothermophilus 균주들은 높은 단백질분해효소활성을 보이면서 다당류 분해와 단당류 이용도가 낮았고, 알칼리환경을 최적 서식지로 하는 G. kaustophilus 등은 이와 대조적으로 지질 분해 및 당류의 이용도가 높게 나타났다. 따라서, 수변 낙엽퇴적층에는 호열성 내성포자 형성 세균인 Geobacillus 속 균주들이 다양한 생태학적 지위를 가지며 퇴비화와 유사한 천이과정을 거치면서 수변 퇴적물질을 분해하는데 기여하는 것으로 보여진다. To understand contribution of thermophilic microorganisms in decomposition of litter deposits on shore of lakes, we surveyed a lakeshore litter deposit for bacteria growing at $60^{\circ}C$. Ten thermophilic isolates were selected for in-depth characterization, based on their high capacity to degrade high molecular weight organic compounds. Based on phylogenetic analysis on their 16S rRNA gene sequences, all isolates were identified as Geobacillus. The optimal growth temperature and pH of the strains ranged $55{\sim}60^{\circ}C$ and 6.0${\sim}$8.0, respectively. Salinity was inhibitory to the growth of the isolates, showing marked decrease of growth rates at 3% salinity. Based on activities of hydrolytic enzymes and profiles of carbohydrate utilization (determined by API 50 CHB kit), three G. stearothermophilus strains showed patterns clearly distinctive from other isolates. Two G. kaustophilus strains also demonstrated distinctiveness in their metabolic pattern and ecological parameters. However, ecological and metabolic profiles of the other five isolates were more variable and showed some degree of digression from their phylogenetic classification. Therefore, it could be concluded that endospore-forming thermophilic bacteria in lakeshore litter deposits contribute to degradation of organic materials with diverse ecological niches while having successions similar to microbial flora in compost. We propose that the thermophilic isolates and/or their thermo-tolerant enzymes can be applied to industrial processes as appropriate mixtures.