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중학생의 체육수업 참여태도가 신체적 자기개념에 미치는 영향
백인국,박종률 한국교원대학교 학교체육연구소 2012 학교체육연구소지 Vol.19 No.1
This research was designed to illuminate that the participation of students in physical education classes have the positive impact on the mental aspects by analyzing how the participation attitude has influence on physical self-concept. The objects of thi
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돌가자미(Platichthys bicoloratus) 암컷과 강도다리(P. stellatus) 수컷 간 교잡종 종자 생산을 위한 최적 사육 수온
백인국,박종연,김동수,방인철 한국수산과학회 2023 한국수산과학회지 Vol.56 No.1
Interspecific hybridization is a beneficial strategy in aquaculture. In this study, diploid hybrids were produced through interspecies hybridization of flounder Platichthys bicoloratus ♀ × starry flounder P. stellatus ♂, which became commercial within a year. These hybrids exhibited an advantage in growth ability, with female hybrids growing twice as large as males at 11 months. Furthermore, hybrid flounders exhibited faster growth rates than starry flounders and reciprocal hybrids. The average growth rate of hybrids was 1.37 and 1.18 times greater than that of starry flounder and reciprocal hybrids at 5.5 months, respectively; Additionally, feed efficiency was 1.21 and 1.10 times higher than that of the experimental groups. The experiments were conducted at five temperatures to determine the optimal fry production method. Six weeks of culture resulted in a significant temperature effect, with maximum growth at 27°C. However, over 90% of the males occurred in the groups at temperatures above 24°C. Therefore, the optimum temperatures for survival, growth, condition factor and frequency of the best-sized fingerling at 13 weeks of age were estimated at 21°C. Thus, hybrid flounders are of great potential for use in the flounder aquaculture industry.
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Multiplex PCR을 이용한 돌조개상과 (Bivalvia: Arcoidae) 이매패류 4종의 신속한 종 판별법 개발
김용휘,한호섭,윤봉한,박종연,백인국,방인철 한국패류학회 2021 The Korean Journal of Malacology Vol.37 No.4
A multiplex PCR primers (the species-specific primers) set using molecular biological methods targeting four species of ark shells (Anadara kagoshimensis, Tegillarca granosa, Anadara broughtonii and Cucullaea labiata) distributed in Korea were built, and a rapid and accurate species identification method was developed. The species-specific primer was designed within the nucleotide sequence of about 573 bp corresponding to the cytochrome c oxidase subunit 1 (co1) gene region of mitochondrial DNA, and considering the single nucleotide polymorphism (SNPs) representing inter-species variation excluding intra-species variation, it was designed with an interval of 80-100 bp for each species. As a result, it was confirmed that species-specific bands were formed in the order of A. kagoshimensis (160 bp), T. granosa (245 bp), A. broughtonii (351 bp), and C. labiata (471 bp). In addition, in order to measure the limit of multiplex PCR amplification according to the concentration of genomic DNA (gDNA) and the number of PCR amplification repetitions, 15 experimental groups were divided and measured. As a result, when the number of amplification repeats was 25 cycles, the concentration of gDNA was detected down to a minimum of 1 ng/μl. Therefore, the multiplex PCR primer set for four species of ark shells developed in this study is expected to be of great help in accurately and rapidly identifying species in the field, it is thought that economic damage and academic errors caused by misidentification can be prevented.
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