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카드뮴(Cd)으로 처리한 백서 섬유모세포와 신경아교세포에서 페놀산에 의한 수복효과
정병호,백승화,한두석 원광대학교 치의학연구소 1995 圓光齒醫學 Vol.5 No.2
Cadmium is a soft, ductile metal which is obtained as a by-product from the smelting of lead and zinc ores. The principal use of cadmium is as a constituent in alloys and in the electroplating industry. Most of all cadmium salts are hazardous. As in human the case with many metals, human exposure may result from both occupational and environmental sources. The general population is mainly exposed to cadmium through food, although tobacco smoking may provide a significant additional source of cadmium. The elumination of cadmium from the body is very slow, so cadmium accumulates over the lifetime. The long term sequela of acute or chronic cadmium exposure includes testicular atrophy, emphysema, renal tubular necrosis, anemia, cardiovascular effects, teratogenic effects, liver damage and osteomalacia. Recently, the hepatic protective effects and enhance the exicision-repair system probably by activating the repair-enzymic or by interacting with DNA and the inhibitory effects of neoplasia on phenolic compounds were studied. This study was carried out to develop compounds repairing rat fibroblast and neuroglial cells damaged by cadmium in vitro. The experimental groups devided into six groups in caffeic acid, chlorogenic acid and tannic acid, respectively; control group was cultured in the media only, NR_50 and MTT_50 groups were cultured in the media .containing NR_50 of cadmium and four experimental groups were cultured in the media containing NR_50 of cadmium and various concentrations of caffeic acid, chlorogenic acid and tannic acid. The 5.0×10^4 cell/㎖ of rat fibroblasts and neuroglial cells in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution of all groups. After the fibroblast and neuroglial cells of all groups were cultured in same condition for 48 hours, neutral red(NR) and tetrazolium MTT(MTT) of the colorimetric assay were performed to evaluate the cytotoxicity of cell organelles. And the lactate dehydrogenase(LDH) activity was measured for membrane toxicity. The light microscopic study was carried out to observe morphological changes of cultured rat fibroblast and neuroglial cells. The results were as follows: 1. According to the NR absorbance, the effects of 50μM phenolic acids were active in fibroblast. 25μM caffeic acid and tannic acid and 50μM chlorogenic acid had good repaired effects in neuroglial cells, but 100μM phenolic acids were decreased effects again. 2. By means of MTT absorbance, the repaired effects of 50μM phenolic acids were active in fibroblast and neuroglial cells. However, 100μM phenolic acids were decreased repaired effects again. 3. In LDH activity, 50μM caffeic acid and tannic acid had good repaired effects in fibroblast. Also, 25μM tannic acid and 50μM caffeic acid and chlorogenic acid had good repaired effects in neuroglial cells. But 100μM phenolic acids were decreased repaired effects again. 4. The cell numbers and cell shapes were repaired in the 25μM and 50μM phenolic acids. But 100μM of all acids showed degeneration again. These results should be suggest that phenolic acids(caffeic acid, chlorogenic acid and tannic acid) decreased the cytotoxicity of cadmium in cultured rat fibroblast and neuroglial cells.
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