http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
위궤양조직으로부터 분리된 Helicobacter pylori의 유전적 다양성
김양호 ( Yang Ho Kim ),신명근 ( Myuong Geun Shin ),백근식 ( Keun Shik Baik ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Genetic diversity of Helicobacter pν ro1i isolated from ulcer tissue were determined using RAPD-PCR. Genomic DNAs extracted from pure isolates containing ureA gene were amplified with random primers of OPA-07, OPA-10, OPA-11 , and OPA-12. Amplified DNA products were compared, and their relationships were presented as similarity and dendrogrm. Similarity levels of isolates varied from 0.23 to 0.9 1. Twenty isolates were grouped as 3 domains at the similarity level of 0.6.
HeJicobacter pyJori의 신속한 진단을 위한 연구
김양호 ( Yang Ho Kim ),신명근 ( Myuong Geun Shin ),백근식 ( Keun Shik Baik ),송계민 ( Kye Min Song ),임준영,성치남 ( Chi Nam Seong ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Optimal conditions for the rapid diagnosis of Helicobacter pνlori were determined. Bacterial strains were isolated from biopsy where the u1cer was observed with endoscope. Morphological and cultural properties of the isolates were determined. Efficiency and srnsitivity of several diagnostic tests were compared. U1cer tissues as well as pure cultures of H. pνlori showed positive reaction within 5 min in urease test broth or semi-solid agar containing 10% urea and 0.1% phenol red. Urease test was highly reproducible and sensitive. Urease A gene extracted from pure culture and tissue was amplified by nested PCR using HEPY 1/2 and HEPY3 / 4 primers. Electrophoresis of PCR product confirmed that DNAs extracted from tissues and isolates which show positive reaction in urease test contain Urease A gene. Among several diagnostic methods used in general, urease test was recommended as a convenient, cheap and easy method.