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White spot syndrome virus (WSSV)의 VP28에 대한 단클론 항체 생산
방지형 ( Ji-hyeong Bang ),김위식 ( Wi-sik Kim ),김춘섭 ( Choon-sup Kim ),김종오 ( Jong-oh Kim ),오명주 ( Myung-joo Oh ) 한국어병학회 2019 한국어병학회지 Vol.32 No.1
We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against recombinant VP28 structural protein (rVP28) of white spot syndrome virus (WSSV). We established six hybridoma clones secreting MAbs against rVP28: 15A11, 20G6, 31H2, 34H6, 38D1 and 43A1. All six MAbs recognized the 25 kDa of protein in gill homogenates of WSSV-infected shrimp by western blot analysis, while no reactivity was observed in gill homogenates of normal shrimp. Moreover, high enzyme-linked immunosorbent assay (ELISA) optical density (OD) values (0.8-2.68) were observed in the hemolymphs from WSSV-infected shrimp, while low OD values (less than 0.24) were recorded in the hemolymphs from normal shrimp, by using these six MAbs produced in this study. These results suggest that these six MAbs are useful for the detection of WSSV.