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넙치 자어 사육에 있어서 먹이 종류에 따라 고밀도로 배양한 rotifer 의 먹이효율
박흠기(Huem Gi Park),이균우(Kyun Woo Lee),이상민(Sang Min Lee),김성구(Sung Koo Kim),이종화(Jong Ha Lee),임영수(Young Soo Lim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.2
This study was carried out to investigate the effect of rotifer fed the different diets in high density culture on larval flounder Paralichthys olivaceus. Rotifer was enriched with enrichment supplements, Marine α and ω-yeast for 6 hours after being cultured with freshwater Chlorella for 18 hours during high density culture before it was fed to larval flounder. And rotifer was culutured with marine Chlorella and freshwater Chlorella for 24 hours during semi-continuous high density culture before it was fed to larval flounder. Culture tanks (2 ℓ working volume) set for rotifer culture in a water bath (28℃) were continuously supplied with oxygen gas. The content of n-3 HUFA to fatty acids in rotifer (dry weight %) enriched with Marine α for 6 hours and cultured with marine Chlorella for 24 hours were higher than that in rotifer enriched with ω-yeast for 6 hours or cultured with freshwater Chlorella for 24 hour. The growth and survival rates of larval flounder fed on rotifer enriched with Marine α for 6 hours and cultured with marine Chlorella for 24 hours were higher than those of larval flounder fed on rotifer enriched with ω-yeast for 6 hours or cultured with freshwater Chlorells for 24 hour. And the content of n-3 HUFA of larval flounder fed on rotifer enriched with Marine α for 6 hours was higher than that of larval flounder fed on other rotifers. The results from this study indicated that rotifer culture with marine Chlorella would be suitable for the high density culture and effective diet for the growth of larval flounder.
공기 및 산소 공급과 pH 조절에 따른 rotifer 의 성장과 고밀도 배양의 생산성
박흠기(Huem Gi Park),이균우(Kyun Woo Lee),김성구(Sung Koo Kim) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.6
The growth of rotifer, Brachionus rotundiformis was evaluated at different culture coaditiona. Rotifer was fed on condensed freshwater Chlorella. The productivity of rotifer in the high density culture system was compared to that of 개tifer in the batch culture system, in which rotifer was fed on baker`s yeast. The growth rate of rotifer increased as temperature increased in the culture system supplied with air or oxygengas. The maximum density of rotifer in the culture systems supplied with or was in range of 16,300∼17,000 ind./㎖ at 24℃. In the culture systems supplied with oxygen gas, it ranged 26,300∼30,500 ind./㎖ at 28℃. When the concentration of dissolved oxygen in the culture system supplied with air reached to below 1 ppm or when the concentration of undissolved ammonia in the culture system supplied with oxygen gas reached 16.6∼22.6 ppm, the growth of rotifer decreased. When oxygen gas was supplied and pH was adjusted to 7, the maximum density of rotifer reached to 43,100 ind/㎖ at 32℃. The production costs for 10 billion rotifer in the high density culture and batch culture were 693,000 and 961,000 won, respectively. Therefore, it was concluded that the productivity of rotifer in the high density culture was higher than that in a batch culture.
먹이종류에 따른 Rotifer , Brachionus calyciflorus Pallas 의 내구란 생산 및 부화율
박흠기(Huem Gi Park),권오남(O Nam Kwon),박기영(Kie Young Park),김광양(Kwang Yang Kim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.3
This study was performed to investigate production and hatching rate of the resting egg of freshwater rotifer, Brachionus calyciflorus fed the 5 different diets (ESP, freshwater Chlorella, baker`s yeast, the mixture of 70% ESP and 30% freshwater Chlorella, and the mixture of 30% baker`s yeast and 70% freshwater Chlorella). The highest productions of resting eggs were 123.3 eggs/㎖ and 126.7 eggs/㎖ in the mixture of 70% ESP and 30% freshwater Chlorella, and the mixture of 30% baker`s yeast and 30% and 70% freshwater Chlorella, respectively, which were significantly better than ESP, freshwater Chlorella or baker`s yeast alone (P<0.05). Hatching rate of the resting eggs from rotifer fed the mixture of 70% ESP and 30% freshwater Chlorella was the highest, 71.9% although this value was not significantly different compared with the mixture of 30% baker`s yeast and 70% freshwater Chlorella, and freshwater Chlorella (P<0.05). The results showed that the mixture of baker`s yeast or ESP with the freshwater Chlorella seemed to be the best feeding regime for the production and hatching rate of resting egg of freshwater rotifer.
고밀도 배양에 있어서 영양강화 방법 및 종류에 따른 rotifer 의 지방산 조성의 변화
박흠기(Huem Gi Park),이균우(Kyun Woo Lee),이상민(Sang Min Lee),김성구(Sung Koo Kim),김형선(Hyung Sun Kim) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.6
This study was carried out to compare the growth and fatty acids composition of the rotifer (Brachionus rotundiformis) cultured in high density by the various enrichments and culture methods. The rotifer fed on condensed freshwater Chlorella was enriched with ω-yeast, Algamac, Suaper Selco and marine Chlorella. In another culture method, the rotifer was cultured with enrichment supplements for 6 hours after feeding with condensed freshwater Chlorella supplement for 18 hour. The rotifer fed with condensed marine Chlorella for 24 hours without freshwater Chlorella was used as a control group. Culture tanks (5 ℓ working volume) was immersed in a water bath (28℃). The density of rotifer and dissolved oxygen level in water was stable in control group of rotifer cultured with condensed marine Chlorella for 24 hours and the n-3 HUFA content of rotifer was the highest among the rotifer culture methods. However, the density of rotifer and dissolved oxygen level in the groups of rotifers enriched with ω-yeast, Algamac and Super Selco by methods were drastically decreased. The n-3 HUFA contents of rotifers enriched by Super Selco were higher than those of rotifer enriched by either ω-yeast or Algamac in both methods. The results from this experiment indicated that supplementation of condensed marine Chlorella for 24 hour by the semi-continuous culture was effective for the improvement of the nutritional value of rotifer and it could provide the stable growth condition for rotifer culture in high density.
수온에 따른 담수산 Rotifer , Brachionus calyciflorus Pallas 의 성장과 내구란 생산
박흠기(Huem Gi Park) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.5
The specific growth rate and production of resting egg of the freshwater rotifer, Brachionus calyciflorus Pallas was inrestigated at the different temperatures (20∼30℃). The rotifer was cultured in 250 ㎖ flask and fed on concentrated freshwater Chlorella. Specific growth rate of B. calyciflorus showed higher rate at higher temperature, but maximum density was observed higher at lower temperature, expect at 20℃. The production of resting egg with temperature was showed decrease on the basis on 26℃. The highest number of resting egg per ㎖ and rotifer 10^4 were 157 eggs and 810 eggs at 26℃, respectively. This result shows that the optimum temperature for mass culture and resting egg production of this freshwater rotifer were 30℃ and 26℃, respectively.
먹이종류에 따른 Rotifer , Brachionus rotundiformis 의 고밀도 배양
박흠기(Huem Gi Park),김성구(Sung Koo Kim),박기영(Kie Young Park),박영제(Young Je Park) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3
The experiment was carried out in a 10ℓ vessel in order to evaluate the growth and nutritional quality of rotifer, Brachionus rotundiformis fed by different diets(Freshwater Chlorella, Marine Chlorella and ω-yeast) for the high density cultivation. The maximum, densities for the rotifer fed on the marine Chlorella, freshwater Chlorella and ω-yeast were 10,900∼12,400, 9,190∼10,600 and 2,390∼2,750 inds./㎖, respectively. Therefore the maximum densities for the rotifer fed on the marine Chlorella and freshwater Chlorella were highter than that for rotifer fed rotifer fed on the ω-yeast. The essential n-3 highly unsaturated fatty acid in rotifer fed on the marine Chlorella was 8.71% which was slightly lower than that in rotifer fed on the ω-yeast, 9.14%, while it was highter than that in the rotifer fed on freshwater Chlorella, 4.45%. This result indicated that marine Chlorella could be appropriate diet for the high density cultivation of rotifer.