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RISS 인기검색어
- 검색키워드
- 저자 : 박최규 ( Choi Gui Park ) (검색결과 2 건)
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조우영,조성환,박최규,김재훈,현방훈,윤용덕,권창희,Cho, Woo-young,Cho, Sung-whan,Park, Choi-gui,Kim, Jae-hoon,Hyun, Bang-hoon,Yoon, Yong-dhuk,Kweon, Chang-hee 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
This study was conducted to elucidate the distribution of Aujeszky's disease viral nucleic acids and antigens in the central nervous system (CNS) of piglets. The first Korean isolate of Aujeszky's disease virus(ADV) that isolated from naturally infected piglets in Yang San, was inoculated into 32 day old piglets with $10^{5.9}TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at every 24hrs for 8 days. The immunohistochemistry (IHC) was conducted to detect the viral antigens in paraffin-embedded tissue sections using avidin-biotin-peroxidase complex (ABC) method. The viral nucleic acids were detected by in situ hybridization (ISH) using ADV specific DNA probe labeled with digoxigenin. The ADV antigens were detected in reticuloendothelial cells of spleen, lymph nodes and tonsil, alveolar walls, leptomeningeal vascular walls, inflammatory foci of each organ, and nerve cells. The viral nucleic acids were detected in the spinal trigeminal nucleus and its tracts of the pons and medulla oblongata by the ISH technique. The pathways of AD viruses in CNS were determined by IHC and ISH. In the intranasally inoculated group, the viruses in nasal mucosa moved to medulla oblongata and pons through the trigeminal nerve. In case of intramuscullarly inoculated group, viruses moved to brain via lymphoid organs or spinal nerves from sciatic nerves.
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Aujeszky`s disease virus 국내분리주 접종자돈의 병리발생에 관한 연구: 1. 병리조직학적 및 전자현미경적 관찰
조우영 ( Woo Young Cho ),조성환 ( Sung Whan Cho ),김재훈 ( Jae Hoon Kim ),박최규 ( Choi Gui Park ),황의경 ( Eui Kyung Hwang ),조부제 ( Boo Jue Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.1
This study was conducted to elucidate the pathogenesis of Aujeszky`s disease virus(ADV) by histopathologic examination. The first Korean ADV isolate, which was isolated from piglets with clinical signs of Aujeszky`s disease in Yangsan(YS) county, Kyungnam province, was inoculated into 32 days old piglets with a dose of 10(5.9)TCID50/mℓ through intranasal or intramuscular route. These piglets were sacrificed at intervals of every 24hrs for 8 days. The virulence of YS strain was determined by the observation of clinical signs, gross findings, and histopathologic changes in tissues. The virus recovery test was performed from brain, spleen, lung and tonsil in cell culture. The pathogenesis of YS strain was determined by the observation of histopathologic lesions in CNS and neurorial tracts. The major clinical signs were fever, anorexia, dyspnea, constipation, tremor, ataxia, circling movement, hindleg paralysis and salivation. The clinical signs were more severe in piglets of the group inoculated intranasally than those of the intramuscularly inoculated gorup. Lymphocytopenia was detected on day 5 to day 6 postinoculation (PI). The ADV was recovered from the tissue homogenates of tonsil, lung, spleen and cerebrum in cell culture. The highest virus titer was detected from tonsil between day 6 and day 7 PI. Reddish sublobar consolidation foci were scattered in the apical and cardiac lobes of lung. Although yellowish necrotic foci were detected in tonsil and liver, hemorrhagic lesions were mainly observed in heart, kidney and lymph nodes. Histopathologically, degeneration and necrosis of nerve cells, nonsuppurative meningoencephalitis, nodular gliosis and perivascular cuffings were observed in CNS. Multifocal fibronecrotic foci were observed in lung, liver, lymph nodes and spleen. The major pathologic changes were detected in the midbrain, pons and medulla oblongata. Eosinophilic intranuclear inclusion bodies were mainly observed in epithelia and/or macrophages of tonsil, liver, lung, spleen and submandibular lymph nodes, and neurons of brain, respectively. Observation of viral particles at various stages of replication were possible from the endothelial cells of the alveolar capillaries and tonsillar crypt epithelia by transmission electron microscope.
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