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      저자 : 박연경 ( Yeon Gyeong Park ) (검색결과 1 건)
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        연꽃의 산화적 DNA 손상 억제 활성 및 항염증 효과

        정형진 ( Hyung Jin Jeong ),박연경 ( Yeon Gyeong Park ),장태원 ( Tae Won Jang ),김도완 ( Do Wan Kim ),정진부 ( Jin Boo Jeong ),박재호 ( Jae Ho Park ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.3

        Objective : Nelumbo nucifera , its rhizome and semen have been used as a traditional medicine which was studied on antioxidant, hepatoprotective effect, anti-obesity and the others. However, Nelumbinis Flos have not studied. We investigated protective effects on oxidative DNA damage and anti-inflammatory effects of Nelumbinis Flos. Methods : The antioxidant activity was conducted by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2`-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay and reducing power assay. Total phenolic content was analyzed. Also, phenolic compounds were detected by HPLC/UV. The inhibitory effect on oxidative DNA damage was determined using ΦX-174 RF I plasmid DNA cleavage assay. The anti-inflammatory effect of Nelumbinis Flos was measured by the amount of nitric-oxide (NO) produced and protein levels of iNOS, and COX-2 in LPS induced RAW 264.7 cells. Results : The results of DPPH and ABTS radical scavenging activity at 200 ㎍/㎖ of extraction were 97.02±0.88% and 96.42±0.25%. Reducing power (fold of L-ascorbic acid as control) was 100.14±0.31 at 200 ㎍/㎖. Total phenol content was 8.70±0.02 ㎎/g. Chlorogenic acid, catechin and epicatechin were found by HPLC. Nelumbinis Flos has inhibitory effect in dose-manner against oxidative DNA damage. In addition, it showed the anti-inflammatory effect by suppression of NO production as well as protein levels of iNOS, and COX-2. Conclusion : This study suggested that Nelumbinis Flos showed potential antioxidant and suppression activities of various factors were related in NO produced. Therefore, Nelumbinis Flos as natural plant resources that may help reduce inflammation and alleviate DNA damage.

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