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발효에 의한 라이신(L-lysine) 생산에 관한 연구(1) -라이신 생산균주의 분리 및 라이신 생산조건의 검토-
민태익,권태완,Mheen, Tae-Ick,Kwon, Tai-Wan 한국식품과학회 1971 한국식품과학회지 Vol.3 No.1
전국 각지에서 수집한 토양을 비롯한 기타 시료로부터 배양액중에 lysine을 생산 축적하는 미생물 94주를 분리하여 이를 다시 비교 검토함으로써 lysine생산이 2 mg/ml 이상인 균주 6주를 선정하고 균학적 성질을 조사한 결과 Micrococcus 속 1주, Corynebacterium 속 3주, Brevibacterium 속 2주였다. Corynebacterium sp. 1주 (S-27-12)에 대한 합성 배지에서의 lysine 축적조건을 검토한 결과 glucose 7.5%, urea 0.6%, $KH_2PO_4\;0.2%$, $Na_2HPO_4\;0.05%$, $MgSO_4{\cdot}7H_2O\;0.03%$, $MnSO_4{\cdot}4H_2O\;0.001%$, $FeSO_4{\cdot}7H_2O\;0.0005%$, pH 7.2가 최적이었으며 이 때의 lysine 생산량은 4mg/ml 정도였다. Ninty four strains of lysine producing micro-organisms in culture broth during fermentation have been isolated from soil and other sources. From the comparison of the amounts of lysine produced, 6 strains have been selected as the potentially useful strains, and identified tentatively as Micrococcus sp. (S-16-4), Corynebactcrium sp. (S-27-12, S-281-3, CBY-4) and Brevibacterium sp. (M-6-71, F-629-2), respectively. From the further studies with Corynebacterium sp., S-27-12, its maximum yield was found to be 4mg lysine/ml of synthetic medium, consist of glucose(7.5%), urea(0.6%), $KH_2PO_4(0.2%)$, $Na_2HPO_4(0.05%)$, $MgSO_4{\cdot}7H_2O(0.03%)$, $MnSO_4{\cdot}4H_2O(0.001%)$ and $FeSO_4{\cdot}7H_2O(0.0005%)$ at pH 7.2 and $30^{\circ}C$ after 4 days.
미생물 페니실린 아미다아제에 관한 연구 ( 3 ) Escherichia coli 가 생산하는 페니실린 아미다아제의 정제 및 동력학적 특성
한문희,성백린,김봉희,민태익 ( Moon H . Han,Baik Lin Seong,Bong Hee Kim,Tae Ick Mheen ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3
Penicillin amidase of Escherichia coli was purified abot 3,200 folds by ammounim sulfate fractionation, DEAE-cellulose, CM-cellulose, and Sephadex G-100 colum chromatography. The overall recovery yield was 57%. As compared with the previous purification method, the purification factor and the recovery yield increased about 56 and 1.4 folds, respectively. The purified enzyme was found to be homogeneous in polyacrplamide gel electrophoresis. The molecular weight determined by SDS-gel electrophoresis was 55,000. The specific activity of penicillin amidase for benzylpenicillin was 2.22 μmoles/sec/㎎ protein and the catalytic rate constant was 122 sec^(-1). The optimum pH and temperature were 8. 5 and 50℃, respectively, This enzyme was rapidly deactivated at temperature above 50℃ and pH above 9.0. Almost all of the metal ions did not affect the enzyme activity except for Hg^(+2) ion. The Michaelis constant for benzylpenicillin was 2.9 mM, the substrate inhibition constants for 6-APA and phenylacetic acid were 10 mM and 4.1 mM, respectively A steady state equation based on the four-step ordered reaction with an acyl intermediate was derived. The inhibition modes of both products based on this equation were discussed with respect to the present experimental results.
대두품종(大豆品種)과 선발(選拔) Rhizobium japonicum간의 숙주(宿主) 친화성(親和性)
김창진,이윤,김성훈,유익동,민태익,Kim, Chang-Jin,Rhee, Yoon,Kim, Sung-Hoon,Yoo, Ick-Dong,Mheen, Tae-Ick 한국토양비료학회 1986 한국토양비료학회지 Vol.19 No.4
우수(優秀) 대두접종제(大豆接種劑)로 이용할 균주(菌株)의 선발을 목적으로 국내(國內)에서 선발한 R. japonicum 균주(菌株)들과 현재 대두장려품종으로 보급되고 있는 대두품종들과의 상호(相互) 접종실험(接種實驗)을 통하여 숙주친화성(宿主親和性) 정도를 조사하였다. 얻어진 결과는 다음과 같다. 1. 공시(供試) 근류균주별(根瘤菌株別) 대두품종(大豆品種)과의 친화성(親和性) 정도를 보면 R. japonicum R-214, R-138 균주(菌株)가 근류형성능(根瘤形成能), 질소고정활성(窒素固定活性) 및 총(總) 질소흡수량(窒素吸收量)도 높아 대두품종(大豆品種)과 친화성(親和性) 범위가 비교적 넓었으며 R. japoncum R-224 균주(菌株)는 아주 낮은 것으로 판단되었다. 2. 대두품종별(大豆品種別) 근류균(根瘤菌)과의 친화성(親和性)은 황금(黃金)콩, 장백(長白)콩 등이 높았으며 그 다음이 장엽(長葉)콩, 단엽(短葉)콩, 백운(白雲)콩, 동북태(東北太) 등의 순이었고 방사(放射)콩이 제일 낮았다. 3. 일반적으로 질소고정활성(窒素固定活性)이 우수(優秀)한 균주(菌株)가 열등한 균주(菌株)보다 숙주친화성(宿主親和性) 범위가 넓은 것으로 판단되었다. In order to select suitable strains for utilization as soybean inoculants, the affinity between Rhizobium japonicum strains isolated from the domestic field and the several soybean varieties being distributed as recommended species was investigated by cross-inoculation experiments. The results obtained are as follows: 1. Rhizobium japonicum R-214 and R-138 showed broad affinity range to varies soybean species in terms of the nodule forming ability, the nitrogen fixing activity and the amount of total nitrogen absorption. In contrast, Rhizobium japonicum R-224 showed very low affinity to the same soybean groups. 2. The affinity of Hwang-Keum Kong and Jang-Paeck Kong to the rhizobium strains was the most signigicant, and that of Jang-Yeop Kong, Dong-Buk Tae, Paeck-Woon Kong and Dan-Yeop Kong was also significant. On the other hand, Pang-Sa Kong showed low affinity to the strains. 3. In general, the strains having high nitrogen-fixing activity seemed to possess the broad affinity range to the soybean hosts.