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한국에서 분리한 agrobacterium tumefaciens T7의 특성과 biovar.결정
이윤,김창진,김성훈,유익동,민태익,Rhee, Y.,Kim, C. J.,Kim, S. H.,Yoo, I. D.,Mheen, T. I. 한국미생물학회 1987 미생물학회지 Vol.25 No.1
For the purpose of securing of strains which can be usefully utilized to study symbiosis between Rhizobium and legume plant, A. tumefaciens T7 was isolated and characterized and then subgroup biovar was determined. A. tumefaciens T7 induced smooth tumor like nopaline type one and did not grow at $37^{\circ}C$ and in the presence of 2% NaCl on yeast extract mannitol medium. The strain was able to grow on the New and Kerr selective media and utilize erythritol but not phenylalanine, tryptophan, and tartarate as a sole carbon source. Negative results were obtained from 3-keto-lactose production and oxidase test. The strain produced alkalifrom malonate and citrate and showed acid litmus milk reaction At least two large plasmids were detected in the cell lysate. According to all of these results, it could be concluded that subdivision of isolated strain was biovar 2.
대두근류 추출물의 첨가에 의한 rhizobium japonicum의 비공생적 질소고정
김성훈,이윤,김창진,유익동,민태익,Kim, S. H.,Rhee, Y.,Kim, C. J.,Yoo, I. D.,Mheen, T. I. 한국미생물학회 1986 미생물학회지 Vol.24 No.2
Soybean nodule extract was prepared and tested for the effectiveness in the induction of asymbiotic nitrogen fixation of R. japonicum P-168. A Asymbiotic nitrogenase activity was increased over twice when glutamate was replaced by nodule extract in the induction media. Independently of the induction media, the nitrogenase activity in the assay media was also enhanced by the addition of nodule extract ($100-400{\mu}g$ protein/ml). The amount of ethylene in the assay media reached the highest point after 8 days incubation of R-168 and was decreased thereafter. The growth of R. japonicum R-168 was sensitive to the concentration of nodule extract. As a while, the effect of soybean root extract was not detected both in the induction of nitrogenase activity and in the growth of R. japonicum R-168.