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콩 및 녹두에서 톱다리개미허리노린재[Riptortus clavatus (Thunberg)](노린재목: 호리허리노린재과)의 산란선호성
정진교,서보윤,문중경,박종호,Jung, Jin-Kyo,Seo, Bo-Yoon,Moon, Jung-Kyeong,Park, Jong-Ho 한국응용곤충학회 2008 한국응용곤충학회지 Vol.47 No.4
콩과 녹두을 대상으로 한 두과작물에 대한 톱다리개미허리노린재의 산란선호성을 검정하였다. 톱다리개미허리노린재는 식물체 상부와 잎의 뒷면에 주로 산란하였으며, 포장 내에서는 가장자리와 안쪽에 관계없이 균등한 산란비율을 나타냈고, 산란된 잎의 대부분에서는 한 잎에 한 개의 알이 관찰되었다. 또 콩의 개화전과 개화 후에 산란비율에 차이가 없었다. 한편 여러 유전자원에 대한 산란선호성도 다른 것으로 나타났다. Oviposition preference of the bean bug, Riptortus clavatus to sites on a plant and within a field, to plants at different developmental stages, and to different leguminous germplasms was observed. The insect layed its eggs mainly on the back surface of leaf in the upper half of plant in both observation from oviposition cage and soybean field. The egg number in fields were observed at a statistically-same rate in marginal and inside area, and at a median value of one egg per leaf in oviposited leaves. Full seed stage of soybean hardly affected oviposition preference of the insect. A statistically-significant difference in oviposition to different leguminous germplasms was observed.
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증기화광산란 검출기를 이용한 콩 함유 수용성 탄수화물의 분석
김경하(Gyeong-Ha Kim),황영선(Young-Sun Hwang),안경근(Kyung-Geun Ahn),김기쁨(Gi-Ppeum Kim),김민지(Min-Ji Kim),홍승범(Seung-Beom Hong),문중경(Jung-Kyeong Moon),정명근(Myoung-Gun Choung) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.7
In the present study, a new analytical method was devised for the simultaneous determination of soluble carbohydrates in soybean seeds using high performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD). The limit of quantification (LOQ) for soybean soluble carbohydrates ranged from 5.6~7.6 mg/kg using the HPLC/ELSD method and from 16.2~33.9 mg/kg using the high performance liquid chromatography/refractive index detection (HPLC/RID) method. Therefore, the HPLC/ELSD method was more sensitive than HPLC/RID. The precision values for retention time and peak area of the HPLC/ELSD method were evaluated by inter-day (n=5) and intra-day (n=10) assays using a standard solution. All precision values (CV<2.5%) for soybean soluble carbohydrates were acceptable and fulfilled international acceptance criteria. All linear calibration curves were obtained with a correlation coefficient of R<SUP>2</SUP>>0.999. The contents of soluble carbohydrates for the "Shingikong" (yellow soybean) and "Cheongjakong 3" (black soybean) samples were analyzed using the HPLC/RID and HPLC/ELSD methods. The difference in carbohydrate contents between the two detection methods was significant. Carbohydrate contents in the HPLC/ELSD method were higher than those in the HPLC/RID method. Overall, the HPLC/ELSD method showed satisfactory resolution with a favorable LOQ and reproducibility. Therefore, these results indicate that the HPLC/ELSD method may be applied to determine the contents of soluble carbohydrates in soybean seeds and related food stuffs.
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