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CAE를 활용한 Hg<SUP>2+</SUP>의 초고감도 검출: 최적화된 DNA 및 Double coffee ring 기반 SERS sensor
채경환(Kyunghwan Chai),류준석(Juneseok You),박주형(Joohyung Park),김우창(Woochang Kim),김민우(Minwoo Kim),김치현(Chihyun Kim),박현준(Hyunjoon Park),김가영(Gayoung Kim),박대일(Daeil Park),박유진(Eugene Park),나성수(Sungsoo Na),박진성(Jin 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.11
박현준(Hyunjun Park),박찬호(Chanho Park),류준석(Juneseok You),나성수(Sungsoo Na) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
The global pandemic of the Covid-19 virus has further raised the importance of diagnosing virus infections. The more contagious, the earlier it should be diagnosed. Since only a small amount of viral nucleic acid is initially present, a highly sensitive detection method is required. In this study, we studied a CRISPR based rolling circle amplification(RCA) for highly sensitive detection of nucleic acids. We used the feature that CRISPR combines with a particular nucleic acid sequence and cuts it, while also cutting the surrounding ssDNA. It is designed to cause secondary amplification only when CRISPR is activated by attaching a C3 blocker to the 3 terminal of reverse primer that binds to the RCA product and causes secondary amplification. As a result, it was confirmed that if the target nucleic acid is present, the amplification is higher than that of the conventional RCA after two stages of amplification, and it is also distinct from the negative control.