ISSR 분석에 의한 구례지역 차나무 집단의 유전다양성과 구조

류재혁(JaiHyunk Ryu),문승옥(Seung Ok Mun),배창휴(Chang-Hyu Bae) 한국차학회 2011 한국차학회지 Vol.17 No.1

In this study, ISSR markers were applied to assess genetic diversity and structure analysis of four tea (Camellia sinensis) populations at Gurye region in Korea. Out of 141 loci detected overall, 135 were identified to be polymorphic with a rate of 95.7% in the 60 individuals. The mean of Nei’s gene diversity (h) and Shannon’s information index (S.I) were 0.230 and 0.350, respectively. AMOVA indicated that 20.4% of the variation was due to the between population and the remaining 79.6% to the within population. The values of total genetic diversity (H_t), average gene diversity within population (H_s), and proportion of total genetic diversity partitioned among populations (G_ST) by Nei’s (1978) method were 0.284, 0.229, and 0.194, respectively. Interpopulation gene flow (Nm) of 2.06 (Nm〉1.0) indicates that genetic exchange occurred among the populations. The average genetic distances among the populations were 0.113 Hwaeomsa, 0.089 Piagol, 0.083 Jirisanplaza and 0.074 Choneunsa, respectively. Based on the cluster analysis of UPGMA, genetic relationship among the four populations was not coincide with the pattern of geographic distribution.

재배지 및 야생지에서 국내 차나무(Camellia sinesis L. O. Kuntze)의 유전적 다양성

배창휴(Chang-Hyu Bae),김길자(Kil Ja Kim),문승옥(Seung Ok Mun),류재혁(JaiHyunk Ryu) 한국차학회 2015 한국차학회지 Vol.21 No.2

In this study, ISSR markers were applied to assess the genetic diversity and structure analysis of tea (Camellia sinesis L. O. Kuntze) among the 3 cultivated populations, a wild population and single variety population (cv. Yabukita) as a control in Korea. Out of 129 loci detected overall, 120 were identified as being polymorphic with a rate of 93.02% in the 50 individuals from the populations. The polymorphic rate of the within population was over (more than) 80% in the four populations, but 16% in the single variety population. The mean of Nei's gene diversity (h) and Shannon‘s information index (S.I.) of the cultivated populations were lower than that of the wild populations. The high values for the total gene diversity, effective and Nei’s genetic diversity indicated substantial variations among the cultivated population. In addition, the insights into the relative gene diversity among and within population of Jeonggeum-ri would be useful in breeding and for the development of strategies for tea genetic population. Based on the cluster analysis of UPGMA, the genetic relationship among the all populations was coincided with the pattern of cultural environment.

한국 돌연변이육종 연구의 역사와 주요 성과 및 전망

강시용(Si-Yong Kang),김상훈(Sang Hoon Kim),류재혁(Jaihyunk Ryu),김진백(Jin-Baek Kim) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Research on mutation breeding started in the early 1960s by researchers at the Atomic Energy Research Institute, Rural Development Administration (RDA) and several universities in Korea. The Radiation Agriculture Research Institute (RARI) was established in 1966, and studies of mutation breeding using radiation were actively conducted for a while. RARI was merged into the Korea Atomic Energy Research Institute (KAERI) and RDA in 1973, and radiation breeding research was neglected by the two agencies. In the 1980s, the relevant research department was lost, which resulted in a recession period of radiation breeding research. The Advanced Radiation Research Institute (ARTI), under the KAERI, was established to promote radiation research and the industry in 2005, which led to the activation of radiation breeding research. Then, the Radiation Breeding Research Center (RBRC) at the ARTI was established with support of the Ministry of Agriculture, Food and Rural Affairs in 2013. Recently, the importance of seed and genetic resources has been emphasized in Korea, and many institutes, companies and private breeders are interested in mutation breeding. The RBRC is trying to develop advanced radiation breeding techniques and new genetic resources using mutation techniques combined with bio-tech. This is to deal with the loss of biodiversity due to global climate change and environmental degradation, growing global demand for food and bio-energy, and to strengthen the protection for new plant varieties. Approximately 180 new mutant varieties were developed and registered officially in Korea. Recently, new mutant varieties, especially of flowers and ornamental plants, have quickly increased and are being commercialized, mainly by private company and breeders.

수수 및 단수수 [Sorghum bicolor (L.) Moench] 수집 유전자원의 내염성 비교 평가

김정민(Jung Min Kim),유재일(Jae Il Lyu),류재혁(Jaihyunk Ryu),김동건(Dong-Gun Kim),이민규(Min-Kyu Lee),김진백(Jin-Baek Kim),하보근(Bo-Keun Ha),안준우(Joon-Woo Ahn),권순재(Soon-Jae Kwon) 한국육종학회 2020 한국육종학회지 Vol.52 No.1

In order to investigate salt-tolerant sorghum germplasms that can grow in saline soil from newly reclaimed land, we measured a well-established germination rate, and growth characteristics including leaf number, height, and root length in salt-treatment conditions (0.3, 0.6, 0.8, and 1.2%) and untreated control. The highly salt-tolerant sorghum line was confirmed using PCA (principal component analysis) analysis and fuzzy comprehensive evaluation method. Germination rate gradually decreased at doses higher than 0.3%, but the germination rates reached about 70% in IT124115, IS1041, Dansusu4ho, and Dansusu2ho germplasms. At 0.6% salt-treatment condition, the germination rates ranged from 35% to 100%. Only seven germplasms (IT103274, IT101381, IT104110, Dansusu4ho, IS20740, IS22720, and IS27887) had germination rates exceeding 50% at 0.8% salt-treatment. At 1.2% salt-treatment IT124115, IT028385, and IS1041 withered. The total number of leaves decreased similarly for both germplasms at salt levels below 0.6%, and sweet sorghum leaf count was more susceptible than grain sorghum at doses higher than 0.8%. In addition, the height of both germplasms was severely reduced even at low salt concentrations, whereas grain sorghum exhibited a greater sensitivity to salinity stress in terms of root length, while sweet sorghum had longer roots at low concentrations when compared with the untreated control. PCA analysis and fuzzy comprehensive evaluation showed that 29 sorghum accessions could be divided into 3 groups based on the germination rate and morphological traits. Especially, sweet sorghum accessions showed a different pattern of PCA plot when compared with the grain sorghum, and salt tolerance could be divided into 5 groups using MFV in terms of their traits. Taken together, the results from this work will contribute to the development of domestic agriculture utilizing marginal land such as reclaimed land by selecting elite sorghum germplasms that have a high salt tolerance and capacity.

국내 채종이 가능한 케나프(Hibiscus cannabinus L.) 신품종 ‘장대’

강시용(Si-Yong Kang),권순재(Soon-Jae Kwon),정상욱(Sang Wook Jeong),김진백(Jin-Baek Kim),김상훈(Sang Hoon Kim),류재혁(Jaihyunk Ryu) 한국육종학회 2016 한국육종학회지 Vol.48 No.3

Kenaf (Hibiscus cannabinus L.) is an annual herbaceous crop of the Malvaceae family. Recently, kenaf is being used in many diversified applications such as pulp, animal feed, bioplastics and cellulosic biofuel etc. A new cultivar Jangdae was developed by mutation breeding using irradiated with 300 Gy gamma-rays. Jangdae has a few distinguishable characteristics such as early flowering, high seed yield and palmate leaf, compared to wild type (Jinju). The fresh matter (FM) and dry matter (DM) yield of Jangdae are similar to those of Jinju, their seed yield (833.3 kg/ha) is approximately 4 times higher than that of Jinju. In addition, the FM and DM yield of Jangdae are 40% higher than those of early-maturing cultivar (C11). We performed the nutritive value of four kenaf cultivars (Jangdae, Jinju, C11 and Hongma300) at 100 day after seeding. The crude protein content of Jangdae, Jinju, C11 and Honma300 were 10.5, 11.0, 9.4 and 10.6%, respectively. The crude fiber, neutral detergent fiber and acid detergent fiber contents were no significant difference among the cultivars. Jangdae, which afforded both a high biomass and seed yield in South Korea, may be useful as potential source of feed and industrial materials.