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류영욱(Young Wook Ryoo),정재봉(Jae Bong Jung),최윤애(Yoon Ae Choi),노용지(Young Ji Rho),이규석(Kyu Suk Lee),송준영(Joon Young Song) 대한피부과학회 1993 대한피부과학회지 Vol.31 No.5
Background : Vitiligo is a relatively common acquired depigmentating disorder that affects at least 1 % of the population. The mode of transmission has not been clearly,stablished, but either polygenic or autosomal dominant with incomplete penetrance and variable expresion has been proposed. Objective : Our purpose was to evaluate the causative factor, variable clinical features and current teratment of generalized vitiligo patients. Method : This clinical in vestigation was made with 225 outpatiens of generalized type vitiligo who had visited the Department of Dermatology, Keirnyung Universitv Hospital from January 1987 till December 1991. Results & Conclusion : 1. There were 125 female, (55.5%) and 100 males(44.4%). 2. The mean age of onset was 21.4 years(male . 24.3, female: 19.1). 3. The mean age at the first visit was 27.5 years (male : 28.8, female : 26.5). 4. The mean duration of the disease was 10.7 years(male : 9.9, female : 10.9). 5. The mean interval between onset and visiting was 6.1 years (male : 4.5, female : 7.4). 6. The most common sites of involvernent at the first visit was tie face 11.7% (male : 11.4%, female : 11.8%). 7. The most common precipitating factor was trauma(8.8%). 8. The most common previous treatment was sunlight irradiaion after application of topical oxoralene(33.2%). 9. The most common degree of depigmented lesion was 5-10%(64.4%). 10. The most common occupations of the patients were students mals, and housewife females. (Kor J Dermatol 1993;31(6):684-671)
류영욱(Young Wook Ryoo),이규석(Kyu Suk Lee),송준영(Joon Young Song) 대한피부과학회 1993 대한피부과학회지 Vol.31 No.5
Background : Recent clinial observations have suggested that colchicine, which is in frequent use in gout can affect the conneciive tissue metabolism in skin and other ti.ues. Objective : This study suggest that further development of colchiine might provide a novel means of modulating collagen gen expression in patients with fibrotic disease. Method : We examed the effect of colchicine on the expres, on of collagen, fibronectin and collagenase gene by skin filroblast culture hy Nort.hern and dot-blot iybridization. Result : The rate of transcription of genomic DNA corresponding o type I collagen and libvonectin was reduced in colchicine-treated cultures but collagenase was not. reduced. Canclusion : The microti.ikile disruptive agent, colchicine, reduced the expression of type I collagen and fibronectin in a dose-(lependent manner. This st.udy suppor that colchicine is one of the promising antifibrogenic drugs curvently being tested as a treatment, of hun an fibrotic disease. (Kor J Dermatol 1993;31(5):696-701)
배양된 각질형성세포에서 Interferon - Υ 가 수포성 유천포창 항원 유전자 발현에 미치는 영향
류영욱(Young Wook Ryoo),신문석(Moon Seok Shin),이규석(Kyu Suk Lee) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.3
N/A Background: Interferon- Υ (IFN- Υ) has been shown to regulate epidermal keratinocyte growth and differentiation and can be isolated from subepidermal vesicles in bullous pemphigoid (BP) patients. In general, the IFN- γ effects ate up-regulatory, and the corresponding transcriptional mechanisms have been elucidated in the case of several genes. One of the markers of the mitotic basal cell phenotype is the expression of BP antigens (BFAG), two hemidesmosomal proteins that were initially recognized as autoantigens in BP. Objective: Since down-regulation of BPA gene expression is one of the earliest events during epidermal differentiation and BPAG is served as the target of autoantibodies, in this study, we examined the effect of IFN-γ on the expression of the gene encoding the BPAG1 and 2. Methods and Results : Northern analysis revealed a dose dependent suppression of BPAG expression by IFN-γ in cultured human skin keratinocytes from 3 different donors, and incubation of the cells with IFN-γ in the presence of cycloheximide demonstrated that this effect reqiured ongoing protein synthesis. Transient transfections of cultured keratinocytes with BPAG1 promoter-chloramphenicol acetyltransferase reporter gene plasmids indicated marked suppression of the promoter activity by IFN-γ. Conclusion : This data, which inactivation of transcription of a basal keratinocyte-specific gene(BPAGl) by IFN-γ, pravides new insight into the mechanisms of IFN-γ mediated keratinocyte gene regulation and epidermal differentitation in inflammatory and blistering skin disease. (Korean J Dermatol 2002;40(3): 235~243)
배양된 피부 각질형성세포에서 nuclear factor kB ( NFkB ) 와 AP - 1 의 활성도에 대한 자외선 B의 효과
구본식,류영욱,이규석 ( Bon Sik Koo,Young Wook Ryoo,Kyu Suk Lee ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.3
Background: Ultraviolet B(UVB) light, which can cause severe damage like induction and promotion of cancer, cutaneous inflammation and immunosuppression, represents one of the most important environmental impacts for humans. Keratinocytes are natural target cells of UVB in humans. NF-kB plays a role in the cell of the immune system, where it controls the expression of various cytokines and the major histocompatibility complex gene. Objective : The purpose of this study was to examine the effect of UVB on the NF-kB and AP-1 activity in cultured human keratinocytes and fibroblasts. Methods : Keratinocyte and fibroblast cultures were produced with DMEM medium. Cells were irradiated for 100J/m, 200J/m, 300 J/m. and Nuclear proteins were extracted. AP-1 and NF-kB activities were measured by the gel shift mobility assay. Results : Gel shift mobility assay. 1. The NFkB activity was increased upon UVB in a dose dependent manner in the keratinocyte. 2. Enhanced levels of AP-1 binding activity in the radiated extracts frorn human skin keratinocytes were detected. 3. The levels of GRE(glucocorticoid responsive element) binding activity were similar in both radiated and unradiated extracts from fibroblasts and keratinocytes. Conclusions : The activities of NF-aB and AP-1 are increased following stimulation of a cell with UVB irradiation. Therefore, UV induced skin tumors, abnormal cell proliferation, cutaneous inflammation and immunosuppression, may be due to these transcriptional factors. (Kor J Dermatol 1997;35(3): 411-417)
인간 피부섬유아세포에서 자외선 B 조사에 대한 Epigallocatechin Gallate 의 영향
서병욱(Byung Wook Seo),서성일(Sung Il Suh),백원기(Won Ki Baek),서민호(Min Ho Suh),김상표(Sang Pyo Kim),정태영(Tae young Jung),조재위(Jae We Cho),김병천(Byung Chun Kim),이규석(Kyu Suk Lee),류영욱(Young Wook Ryoo) 대한피부과학회 2001 대한피부과학회지 Vol.39 No.5
N/A Background : The main polyphenol components in green tee are (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate and (-)-epigallocatechin gallate (EGCG). It is well known that flavonoids such as catechins can be protective against inflammatory and cancer and cardiovascular diseases. These protective effects are largely due to their inhibition of some enzymes and antioxidative activities by scavenging free radicals. Ultraviolet(UV) exposure of the skin, particulary UVB (290-320nm), causes adverse biological effects, including alterations in cutaneous immune cells, photoaging and photocarcinogenesis. Several studies have shown that EGCG afforded protection against UVB-induced inflammatory responses and photocarcinogenesis in murine models. Objective & Methods : In this study, we investigated the effects of EGCG on UVB irradiated human skin fibroblasts using viability test, thiobarbituric acid assay, propidium iodide(PI) stain, and western blot analyses and RT-PCR. Results : Cell survival curves after UVB irradiation showed dose dependent decrement pattern by trypan blue exclusion assay. Only 42% of dermal fibroblasts survived at 150 mJ/cm2 UVB irradiation. The damage was associated with cell membrane lipid peroxidation, as shown by accumulation malondialdehyde(MDA). By pre-cultivation with EGCG (50nmol), a significant preventive effect was noted on the increase in the absolute number of surviving cells(up to 81.5% of cells survived) and the levels of MDA markedly decreased, Morphological changes associated with apoptotic cell death were easily distinguished by PI stain. Bases on our finding, we investgated the regulation of p53, p21, bax, bc1-2, cyclin Dl, E, Cdk2, and PARP proteins by western blot analyses. The expression p53 protein was elevated by following UVB exposure which was inhibited by EGCG treatment. Using RT-PCR, the transcription of p53, fas and jun gene showed similar results which obtained by western blot analyses. Conclusion : EGCG, which have newly accepted as a potential UV protection properties, is effective membrane peroxidation inhibitor and prevent apoptotic changes when present in relevant concentration at the site of action beginning and during UVB irradiation. And the protective mechanism of EGCG against UVB-induced cell damage maybe, at least in part, related with p53, fas and jun pathway.
적자색의 미만성 반점 및 반의 임상을 보인 피부 전이 유방암 1예
김현욱 ( Hyun-wook Kim ),김원오 ( Won-oh Kim ),류영욱 ( Young-wook Ryoo ),김성애 ( Sung-ae Kim ) 대한피부과학회 2021 大韓皮膚科學會誌 Vol.59 No.2
Cutaneous and subcutaneous metastases from the neoplasm of visceral organs are uncommon and have been estimated to occur in only 0.7%∼9% of patients. Among these cases, breast cancer is the most frequently observed malignancy. The incidence of cutaneous metastasis of breast cancer is 23.9%. The most common clinical manifestation of cutaneous metastasis of breast cancer is non-fixed painless erythematous nodules on the anterior chest. However, cutaneous metastasis of breast cancer shows a wide range of clinical manifestations and can mimic benign dermatologic lesions such as erythema annulare centrifugum, contact dermatitis, cellulitis, and erysipelas. We report a 52-year-old woman who presented with diffuse purpuric macules and patches on the right trunk. Finally, based on the clinical and pathologic findings, a diagnosis of cutaneous metastasis of breast cancer was made, and the patient expired 1 year later. (Korean J Dermatol 2021;59(2):140∼143)