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컬러감자 홍영, 자영으로부터 항산화 화합물의 정량 및 간세포 보호 효과 탐색
김상민 ( Sang Min Kim ),강경수 ( Kyungsu Kang ),하인수 ( In Su Ha ),노주원 ( Chu Won Nho ),남정환 ( Jung Hwan Nam ),조지홍 ( Ji Hong Cho ),권오근 ( Oh Keun Kwon ),정진철 ( Jin Cheol Jeong ),이재권 ( Jae Kwon Lee ),판철호 ( Cheol H 충북대학교 과학교육연구소 2014 과학교육연구논총 Vol.29 No.1
The colored Potatoes, ‘Hongyoung’ and ‘Jayoung’ cultivars bred in Rural Development Administration (Korea) contain antioxidant compounds such as chlorogenic acids and anthocyanin compounds in the tuber. In this study, main antioxidant compounds in both cultivars were identified by LC-MS analysis and quantified by HPLC analysis. The result showed that three compounds (petunidin-3-O-coumaroyl-rutinoside-5-O-glucoside, pelargonidin-3-Ocoumaroyl- rutinoside-5-O-glucoside and peonidin-3-O-coumaroyl-rutinoside -5-O-glucoside) were identified as main anthocyanins in ‘Jayoung’ cultivar while only pelargonidin-3-O-coumaroyl-rutinoside-5-O-glucoside was presented as a major anthocyanin in ‘Hongyoung’ cultivar. Among chlorogenic acids, 5-O-caffeoylquinic acid was the most abundant in both cultivars. In order to evaluate hepatoprotective activity of these potatoes, the methanol extracts and their serial solvent fractions were prepared by solvent partition and XAD-7 column chromatography method and treated on hepatoma HepG2 cells of which cytotoxicity was induced by tert-butyl hydroperoxide. Among the tested samples, anthocyanin-rich fractions from both cultivars contained the strongest hepatoprotective effect on HepG2 cells. This result indicates that hepatoprotective effect of these fractions can be occurred from anthocyanin.
김영훈 ( Young Hoon Kim ),박은규 ( Eun Gyu Park ),( Dulamjav Batsuren ),( Jamsranjav Ganbaatar ),노주원 ( Chu Won Nho ),판철호 ( Cheol Ho Pan ),이재권 ( Jae Kwon Lee ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.4
본 연구에서는 아직 까지 연구되지 않은 말굽버섯의 면역증강 효과를 설치류 대식세포에서 검증하였다. 연구결과에 따르면 말굽버섯 메탄올 추출물(FFE)의 처리에 의해 대식세포의 산화질소의 생성이 농도 의존적으로 증가되었다. 산화질소의 생성이 증가된 이유는 산호질소의 생성을 유도하는 효소인 iNOS의 발현이 FFE에 의해 증가되었기 때문이었다. FFE는 면역반응에 중요한 cytokine인 TNF-α, IL-1β, IL-6의 생성도 농도 의존적으로 증가 시켰다. 이 같은 FFE의 면역증강 활성은 면역활성을 중계하는 세포 신호전달분자 중 NF-κB와 MAP Kinases의 활성증가에 의한 것임을 확인 할 수 있었다. 본 연구 결과는 말굽버섯의 임상적 적용에 중요한 자료로 활용될 것이며, 만일 말굽버섯으로부터 분리한 단일성분에서 면역증강활성을 검증 하게 된다면 새로운 식·의약소재로서의 가치를 인정 받을 수 있을 것으로 생각된다. In this study, we demonstrated whether the extract of Fomes fomentarius (FFE; FF extract) could be used to stimulate macrophages (RAW 264.7 cells). All four doses of FFE (5, 10, 20, and 40 μg/mL) had no significant cytotoxicity during the entire experimental period. FFE potently increased the production of nitric oxide (NO). Consistent with these observations, inducible NO synthase levels were increased by FFE in a dose-dependent manner. Moreover, FFE increased the production of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in the same cells. These stimulating effects of FFE were found to be caused by the stimulation of phosphorylation of IκBα and MAP kinases (p38,ERK, and JNK). These results suggest that FFE may be used as new agents for wide application in the immune study of mushroom.