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Lactobacillus acidophilus에 의한 콜레스테롤의 흡착
노동욱 ( Dong Ouk Noh ) 한국유가공기술과학회 1996 Journal of Dairy Science and Biotechnology (JMSB) Vol.14 No.2
Cholesterol assimilated by Lactobacillus acidophilus ATCC 43121 was not metabolically degraded in that most of it was recovered with the cells. Cells grown in the presence of cholesterol micelles and bile salts were more resistant to Iysis by sonication than those grown in their absence, suggesting a possible alteration of cellular membranes. Cholesterol assimilation occurred during growth at pH 6.0, the amount of which was more than that by cells grown without pH control. Cholesterol assimilated by cells was recovered in the membrane fractions of cells both grown at pH 6.0 and without pH control. The effect of unsaturated fatty acids on cholesterol assimilation was not clear, since there was no significant (P> 0.05) difference in the amount taken up from micelles prepared using L- -phosphatidylcholine, dioleoyl or L- -phosphatidylcholine, distearoyl. Without Tween 80, little, if any, cell growth or cholesterol uptake was observed. In the presence of 0.05% Tween 80, cholesterol uptake increased dramatically as did growth. However, as the amount of Tween 80 increased beyond 0.05%, cholesterol uptake decreased while the amount of growth remained the same.
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배기연(Gi Yeon Bae),노동욱(Dong Ouk Noh),서형주(Hyung Joo Suh) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.4
본 연구를 통해 스피루리나로부터 피코시아닌 추출효율의 향상을 위해 적합한 효소 선정과 함께 최적의 가수분해 조건을 확립하고자 하였다. 추출에 적합한 효소의 선정을 위해 세포벽 분해효소(cytolase, rapidase, ultraflo L, viscozyme L) 및 단백질 분해효소(collupulin, flavourzyme, protamex)를 이용했으며, 효소 선정 후 최적의 추출조건을 확립하기 위해 효소 첨가량, 기질 농도, 반응온도 및 반응시간의 조건하에서 피코시아닌 추출을 수행하였다. 스피루리나 효소 추출물의 피코시아닌의 함량은 최대흡광도 값을 이용하여 C-phycocyanin(CPC), allophycocyanin(APC) 함량의 합으로 측정하였다. 피코시아닌 추출 시 세포벽 분해효소와 단백질 분해효소를 이용한 가수분해물에서 피코시아닌의 추출효율이 증가했으나, 특히 단백질 분해효소를 이용한 가수분해 시 추출효율이 향상되는 것을 확인했다. 단백질 분해효소 중 collupulin과 protamex에서 추출효율이 높게 나타나 이 두 가지 효소를 비율별로 혼합하여 추출에 사용함으로써 추출효율의 향상을 기대했으나, collupulin을 단독으로 이용하였을 때 총 피코시아닌(CPC+APC)의 함량이 3.97mg/g of dry weight로 가장 높은 추출효율을 나타내었다. 따라서 collupulin을 이용하여 총 피코시아닌 함량을 기준으로 다양한 효소 첨가량, 반응온도와 시간 및 기질 농도 조건하에서 최적의 추출조건을 확인하였다. 그 결과 스피루리나로부터 피코시아닌을 추출하기 위한 최적 가수분해 조건은 5.0% 스피루리나를 기질 대비 3.0%의 collupulin으로 50°C에서 4시간 반응 시 총 피코시아닌의 함량이 유의적으로 증가하는 것을 확인하였다. Collupulin을 이용한 스피루리나 가수분해에 의해 활성성분이 피코시아닌의 추출이 가능해졌으며, 이는 새로운 활성소재로의 활용이 가능할 것이다. This study established the suitable enzymes and optimal hydrolysis conditions for improving the extraction efficiency of phycocyanin from spirulina. Cell wall lysis enzymes (cytolase, rapidase, ultraflo L, and viscozyme L) and proteolytic enzymes (collupulin, flavourzyme, and protamex) were used to select suitable enzymes for extraction. In order to establish the optimal extraction conditions, the changes in the phycocyanin extraction amount were measured under various conditions. The amount of phycocyanin was measured by the sum of the amounts of C-phcocyanin (CPC) and allophycocyanin (APC). The extraction efficiency of phycocyanin increased in the hydrolysates using cell wall lysis enzymes and proteases. Among the hydrolysates, the extraction efficiency was improved when spirulina was hydrolyzed by proteases, collupulin and protamex. It was expected that a collupulin-protamex mixture would improve the extraction efficiency, but the total phycocyanin (CPC+APC) content was the highest at 3.97 mg/g of dry weight when collupulin alone was used. Thus, the study established the optimal conditions for spirulina hydrolysis by collupulin. As a result, it was confirmed that the total phycocyanin content significantly increased under optimum hydrolysis conditions (5% spirulina and 3% addition of collupulin against spirulina) for extracting phycocyanin from spirulina for 4 hours at 50°C. Spirulina hydrolysis with collupulin enabled the extraction of spirulina as an active ingredient, and spirulina could be utilized as a new nutraceutical ingredient.
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담즙이 불가리커스 유산균의 유당분해효소인 β-galactosidase의 활성에 미치는 영향
노동욱 가야대학교 1999 가야대학교 논문집 Vol.8 No.-
In the presence of 0.15% oxgall, the β-galactosidase activity of whole cells of Lactobacillus bulgaricus was increased. However, higher concentrations of oxgall decreased the enzyme activity. In the absence of oxgall, little or no β-galactosidase activity was exhibited by the whole cells. When the cells were lysed to obtain cell-free extracts prior to assay, there was some decrease in the enzyme activity in the presence of 0.15% oxgall. Increasing the concentrations of oxgall did not further reduce the activity. Apparently, the oxgall increases the permeability of the cells up to a certain concentration(0.15%) to allow more substrate to enter, resulting in more hydrolysis of the substrate. However, at the concentrations higher than that, the enzyme activity was inhibited by the oxgall
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Effect of Bile on β-Galactosidase Activity of Streptococcus thermophilus
NOH, DONG-OUK 가야대학교 2001 가야대학교 논문집 Vol.10 No.-
In the presence of 0.15% oxgall. the β-galactosidase activity of Streptococcus thermophilus increased. When the cells were incubated in PMN broth, only one strain (HC11) grew almost twice as much as other 3 strains, meanwhile there was no difference among the strains when they grew in MRS broth. When they were incubated either in MRS broth or in PMN broth with oxgall, the enzyme activity increased for all strains of S. thermophilus tested. Especially, the growth in PMN broth resulted in more increase of enzyme activity than that in MRS broth for all strains tested.
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Influence of Bile on Cellular Integrity and β-Galactosidase Activity of Lactobacillus acidophilus
Noh, Dong-Ouk 가야대학교 2000 가야대학교 논문집 Vol.9 No.-
In the presence of oxgall, the β-galactosidase activities of all strains of Lactobacillus acidophilus was increased. Among them, strain 606 showed to have the highest enzyme activity. Meanwhile, strain 107 did not show any change in the enzyme activity. As for the bile tolerance, all tested strains with the exception of strains NCFM and 606 were tolerant to the presence of oxgall, and among them, strain 4356 was the most bile-tolerant. There was no change in cellular integrity for all strains tested. This means there was no lysis of the cells, but a change in the permeability of cellular membranes of the cells, resulting in letting more lactose into the cells and increasing the enzyme activity.
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