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농법에 따른 메탄생성과 메탄생성 세균의 T-RFLP 패턴
김훈수,조주식,박경량,Kim, Hun-Soo,Cho, Ju-Sik,Park, Kyeong-Ryang 한국미생물학회 2009 미생물학회지 Vol.45 No.1
농법에 따른 토양의 토양 성분과, 메탄생성량, 메탄생성 세균의 수, 그리고 terminal restriction fragment length polymorphism (T-RFLP) 패턴을 계절별로 조사하였다. 조사기간 동안의 대부분 토양 성분은 큰 차이를 나타내지 않았고, 토양내 물 함량은 5월 시료 보다 10월 시료가 높게 나타났다. 그리고 most probable number (MPN) 방법을 이용한 메탄생성 세균 계수에서, 모든 논토양에 메탄생성 세균이 비교적 많이 존재하였으나 수소를 이용하는 메탄생성 세균과 포름산을 이용하는 메탄생성 세균에 비해 아세트산을 이용하는 메탄 세균수가 상대적으로 적은 것을 확인하였다. 또 포름산 이용 실험에서 대부분 토양이 1주부터 빠른 포름산 이용능을 보여, 4주에는 미량만이 검출되었으나, 아세트산을 첨가한 실험에서는 3주까지 아세트산이 증가된 후, 그 이후에야 아세트산이 이용되어 감소됨을 확인하였다. 그리고 수소를 첨가해준 모든 시료에서는 많은 양의 메탄생성이 있음을 확인하였다. 또 Sou96I을 처리한 mcrA 유전자의 T-RFLP 패턴을 분석한 결과, 농법에 따라 토양성분이 크게 차이가 없는 것과 같이 농법과 계절에 의해서도 뚜렷한 차이를 나타내지 않았지만 일부 통계분석 자료는 유의성이 있음을 확인하였다. 따라서 토양 미생물의 군집비교 기법을 미생물학적 지표로 활용할 수 있다고 판단된다. We studied soil components, methane production, the number of methanogens, and T-RFLP patterns dependent on agricultural methods with the change of seasons. There is no regular increase or decrease tendency of the most soil components followed by sampling period. And the water content in soil was higher in October than May. Also a lot of methanogens existed in soil, and acetotrophs were relatively of smaller number than hydogenotrophs and formate utilizing methanogens using MPN (most probable number) enumeration. In the experiment using the formate, it was used from the first week, and only a minute amount was detecte after four weeks. However in the acetate, it was increased until the third week, and after that was consumed. And there was higher methane production for all soil samples which administered with the hydrogen spike. The activity of methanogens was higher in the organic and low-agrichemical agricultural method samples, and the organic agricultural method had high methanogen activity among the other samples. A result of T-RFLP pattern of mcrA gene digested with Sau96I, methanogen community have a little relation with agricultural methods and seasons. This results also agreed to no critical difference the soil components dependent on agricultural methods, but some analytical data have a positive relationship with a agricultural methods. Therefor we could concluded that the comparison study of community for soil bacteria sufficiently could be useful for the microbiological indicator.
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뇌자기공명영상을 이용한 천막 절흔의 형태학적 분석 -임상연구-
김훈수 ( Hoon Soo Kim ),조성민 ( Sung Min Cho ),이호국 ( Ho Kook Lee ),변진수 ( Jhin Soo Pyen ),홍순기 ( Soon Ki Hong ),김헌주 ( Hun Joo Kim ) 대한뇌종양학회 2004 대한뇌종양학회지 Vol.3 No.2
Objective:This study was performed to measure anatomical basis for interpretation of the tentorial notch, oculomotor nerve and brainstem using MRI and to develop a classification system for the tentorial notch. Methods:From January 2002 to September 2002, we analized 100 patients with mild head injury who didn`t have space occupying lesion on the magnetic resonance imaging(MRI). The following measurements were done:1) anterior notch width(ANW);2) maximum notch width(MNW);3) notch length(NL);4) posterior tentorial length(PTL);5) interpedunculoclival( IC) distance;6) apicotectal(AT) distance;and 7) inter-third nerve angle. Quatile groups defined by NL were labeled long, short, and midrange, and those defined by MNW were labeled as wide, narrow, and midrange. Results:The patients were composed of 64 men and 36 women whose mean age was 29.6±15.7 year of age(range 2- 61). Mean values are presented as the means±standard deviations into following variations:1) ANW, 22.3±4.4mm; 2) MNW, 32.9±3.6mm;3) NL, 57.6±5.4mm;4) PTL, 30.4±3.0mm;5) AT distance, 25.2±4.8mm;6) IC distance, 18.0±4.9mm;7) inter-third nerve angle, 50.8±6.9°. The types of tentorial notch were wide(16%), narrow (11%), long(12%), short(12%), and typical(17%). Notches that were both wide and long were labeled large(8%), and those that were narrow and short were labeled small(12%). The category of mixed notches(12%) was assigned to tentorial notches that were either wide/short(4%) or narrow/long(8%). Conclusions:The use of MRI to identify the type of tentorial notch and regional anatomy may help neurosurgical decision making.
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