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제주 재래마유 단백질에 관한 연구 1 . Casein 의 성장 ( 1 )
이현종,김호구 ( H . J . Lee,H . K . Kim ) 한국축산학회 1985 한국축산학회지 Vol.27 No.6
The properties of Cheju native mares` casein investigated and compared with Throughbred mares` casein by polyacrylamide gel electrophoresis (PAG), Sepharose 6B gel filtration and DEAE-cellulose column chromatography. Cheju native mares` casein separated in main three components, αs-, β-, k-casein by polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. On Sepharose 6B column, 3 fractions were obtained from acid casein: the first with k-, the second with αs-, β-, k-and the thud with mainly αs-, β-casein. Acid casein was fractionated into 5 fractions on DEAE-cellulose column with 0.01 M-Imidazole-HCl buffer (pH 7.0). The major components of the identified fractions are: fraction A, k-casein: B to C, mainly β-cassein: D to E, αs-casein.