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염색공단폐수의 색도처리를 위한 염료분해 균주의 분리와 특성
김정태 ( Jung Tae Kim ),박근태 ( Guen Tae Park ),이건 ( Geon Lee ),강경환 ( Kyeong Hwan Kang ),김중균 ( Joong Kyun Kim ),이상준 ( Sang Joon Lee ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.1
To treat chromaticity contained in effluents of dyeing wastewater efficiently, potent dye-degrading microorganisms were isolated from influent water, aeration- tank sludge, recycle water and settling-tank sludge located in leather and dyeing treatment plant. Six potent strains were finally isolated and identified as Comamonas testosteroni, Methylobacteriaceae bacterium, Stenotrophomonas sp., Kluyveromyces fragilis, Ascomycetes sp. and Basidiomycetes sp. When Basidiomycetes sp. was inoculated into ME medium containing basal mixed-dyes, 93% of color was removed after 8 days incubation. In the same experiment, the 1:1 mixed culture of Basidiomycetes sp. and photosynthetic bacterium exhibited 88% of color removal; however, it showed better color removal for single-color dyes. The aeration-tank and settling-tank samples revealed higher color removal (95-96%) for black dyes. The settling-tank sample also revealed higher color removal on basal mixed-dyes, which resulted in 90% color removal after 6-h incubation. From the above results, it is expected to achieve a higher color removal using the mixed microorganisms that were isolated from aeration-tank and settling-tank samples.
염료 분해균 증대를 통한 Pilot Plant에서의 담체 내 미생물 생태와 색도처리에 미치는 영향
김정태 ( Jung Tae Kim ),이건 ( Geon Lee ),박도현 ( Do Hyeon Park ),강경환 ( Kyeong Hwan Kang ),김중균 ( Joong Kyun Kim ),이상준 ( Sang Joon Lee ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.4
In a pilot-scale dyeing wastewater treatment using two-type fluidizing media, each thickness of biofilm was 15 and 30 μm, respectively. The numbers of protozoa inhabited in small-size (PEMT A) and big-size (PEMT B) media were 7.5 x 10(4) and 1.25 x 10(5) cells/ml, respectively, and dominant species were Entosiphon sulcatus var sulcatus in PEMT A and Chlamydomonas reinhardtii in PEMT B, respectively. Flask experiments using the two media revealed that the percentages of color removal were 25.8% in PEMT A and 27.1% in PEMT B after 72-h cultivation, indicating the necessity of bioaugmentation. Experiments for bioaugmentation effect on color removal were carried out in the pilot-scale treatment for 75 d by three-step operation under the control of wastewater loading rate and microbial input rate. Dye degradation occurred mainly in the second reaction tank, and the attachment of augmented dye-degrading microorganisms to media took at least 35 d. Final value of chromaticity in effluent was 227, meeting the required standard. Therefore bioaugmentation onto media was good for color treatment. In summary, thickness of biofilm formed on the media depended upon the size of media, resulting in different ecosystem inside the media. Hence, this affected microbial community and color treatment further. Accordingly, the reduction of operation cost is expected by efficient color-treatment process using bioaugmented media.
빈정인,이병헌,김중균,권성현,감상규,이민규 한국환경과학회 2001 한국환경과학회지 Vol.10 No.4
A packed bed of volcanic rock was used as deodorizing material to remove hydrogen sulfide(H_2S) from air in a laboratory-scale column, and was inoculated with Thiobacillus sp. as H_2S oxidizer. The effects of volcanic rock particle size distribution on system pressure drop were examined. Various tests have been conducted to evaluate the effect of H_2S inlet concentration and EBCT(Empty Bed Contact Time) on H_2S elimination. The pressure drop for particles of size range from 5.6 to 10 ㎜ was 14 ㎜H_2O/m at a representative gas velocity of 0.25m/s. Biofilter using scoria and Thiobacillus sp. could get the stable removal efficiencies more than 99.9% under H_2S inlet concentrations in the range from 30 to 1,100ppm at a constant gas flow rate of 15.2 ℓ/min. H_2S removal efficiencies greater than 99% were observed as long as EBCT was longer than 8sec at the 250ppm of H_2S inlet concentration. When EBCT was reduced to 5.5 sec, H_2S removal efficiency decreased by about 12 percent. The maximum H_2S elimination capacity was determined to be 269g-H_2S/㎥·hr.
화산석을 담체로 한 Biofilter 에서 황화수소 제거 특성
이병헌,이민규,김중균,권성현,최혁,빈정인 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.3
생물학적 탈취법은 에너지절약과 아울러 유지관리가 용이하여 기존의 물리화학적 처리법에 대한 대체 방법으로 주목을 끌고 있다. 화산석과 Bacillus sp.가 충전된 바이오필터를 이용하여 H₂S 제거에 대한 유입농도와 공탑접촉시간(EBCT, Empty Bed Contact Time)의 영향을 평가하고 최대제거용량 산정을 위한 다양한 실험을 수행하였다. 화산석 입자 크기가 5.6-10 ㎜ 인 경우에 0.25m/s의 유속에 대해 13.4㎜H₂O/m의 압력손실이 측정되었다. 화산석과 Bacillus sp.를 사용한 바이오필터는 유입농도 250 ppmv에서 EBCT 8.2-60 sec의 범위에서 99% 이상의 안정된 제거효율을 유지하였다. 또한 체류시간을 5.5 sec로 감소시킨 결과 H₂S 제거효율은 약 10% 감소하였고 이때의 H₂S 제거용량은 254 g-H₂S/㎥/hr로 산정되었다. 일정한 유입 유량 15.2 L/min(선속도는 0.04 m/sec)의 조건에서 유입농도가 30-1,100 ppmv의 경우 H₂S 제거효율은 99.97% 이상이였다. Biological deordorization receives attracting attention as an alternative method to physical and chemical treatment, mainly because of its energy-saving and easy maintenance features. A biofilter packed with scoria, and inoculated with Bacillus sp. as H₂S oxidizer was used to remove hydrogen sulfide in the air stream. Various tests have been conducted to evaluate the effect of H₂S inlet concentration and EBCT (Empty Bed Contact Time) on H₂S elimination, and to determine the maximum elimination capacity. The pressure drop in the biofilter packed with particles of size range from 5.6 to 10 ㎜ was 13.4 ㎜H₂O/m at a representative gas velocity of 0.25 m/s. Biofilter showed the stable removal efficiencies of over 99% under the EBCT range from 8.2 to 60 sec at the 250 ppmv of H₂S inlet concentration. When the retention time was reduced to 5.5 sec, the H₂S removal efficiency decreased by about 10 percent and the H₂S elimination capacity was 254 g-H₂S/㎥/hr. The removal efficiencies of over 99.9% were observed in the range of inlet H₂S concentration from 30 to 1,100 ppmv at a constant gas flow rate of 15.2 L/min (linear velocity, 0.04 m/sec).