Prednisolone 투여가 원발성 아메바성 뇌수막염의 실험적 발생에 미치는 영향

김옥용,임경일 대한기생충학회 1985 The Korean Journal of Parasitology Vol.23 No.1

Naegleria fowleri의 감염에 의한 생쥐의 원발성 아메바성 뇌수막염의 발생에 prednisolone투여가 어떠한 영향을 주는가를 관찰한 바 다음과 같은 결론을 얻었다. 1. 각 실험군 별로 $1{\times}10^5$의 N. fowleri를 감염시킨 후 생존기간을 관찰하였더니, prednisolone을 투여한 제1군의 평균 생존기간은 10.2일, 제2군은 10.6일, 제3군은은 11.3일로써 prednisolon을 투여한 군이 비투여군에 비하여 유의하게 생존기간이 단축됨을 알 수 있었으며, prednisolon투여군인 제1, 2, 3군 사이에 유의한 차이는 없었으나 전투여군인 제1군에서 생존시간이 단축되는 경향을 나타내었다. 2. 각 실험군 별로 감염 이후 20일까지 체중의 변화를 관찰한 결과, prenisolone 투여군에서는 서로 비슷한 양상을 나타내었는데 투여하는 시기부터 어느 정도 감소하다가 일정한 시간이 지나면서 회복현상을 나타내었다. 또한 prednisolon만 투여한 제 IV군에 비하여 제1, 2, 3군에서 체중이 더욱 심하게 감소하는 현상을 나타내었다. 그리고 대조군은 감염 8일 후부터 감소하기는 하였으나 큰 차이는 없는 것으로 보아 체중감소현상은 주로 prednisolone에 의해 영향을 받는 것으로 보인다. 3. Prednisolone 투여한 감염군은 대조군에 비하여 뇌와 폐조직에서 비교적 심한 병변이 나타났음을 알 수 있었다. 4. 감염된 생쥐의 임상증세는 감염 후 며칠이 지나면 머리를 비비고 고개를 떨구며, 식욕부진이 나타나 먹이를 먹지 않다가 발작이 오고, 빈사상태가 되어 사망하게 된다. 대부분의 생쥐에서 이러한 증세가 나타나는데, prednisolone 투여군에서는 감염 5일 이후부터 증세가 나타나는 경향을 보였고, 대조군은 감염 8일 이후에야 나타나 10일경에 사망하였다. 이상의 결과를 종합항 보면 prednisolone을 투여하여 인위적으로 면역반응을 억제시켜 줄 경우 N. fowleri에 감염된 생쥐의 생존기간은 단축되고 병변도 더욱 심하게 나타나며, 감염전에 투여해 주는 것이 가장 치명적인 영향을 줄 수 있음을 알 수 있었다. Present study aimed to elucidate the immunosuppressive effect of prednisolone on Naegleria fowlsri infection in mice. N. fowleri was cultured in CGVS medium (Willert and Le Ray, 1973) . White female mice, weighing about 18g, used for experiments were divided into five groups; untreated control group, prednisolone treated groups (before, during and after infection), and only prednisolone treated group. In the prednisolone treated group, the hormone was injected intramuscularly 5 doses of 10 mg/kg every other day. According to designated time of treatment, each mouse was challenged with $1{\times}10^5$ N. fowleri intranasally. Changes of body weights, clinical manifestations and number of dead mouse were observed. Brain and lung tissues of dead mice were cultured in the non-nutrient agar (Kasprzak and Mazur, 1972), or stained with hematoxylin.rosin for the examination of histopathological changes. Results of the experiment are summarized as follows: Mortality among the prednisolone treated groups was higher than that in untreated control group, and among the treated groups, the pretreated group showed shorter survival time. Body weights among untreated control mice showed no significant increase, however, treated groups of mice showed the decrease during the administration and recovery of the weights were observed at 2 to 3 days after the completion of treatment. In the treated control groups, the infected mice began to show the pathologic findings 5 days after infection while, the untreated mice began to show the findings 8 days after infection. Tissue damages in brain and lung occurred due to virulence of amoeba were more severe among treated mice than that in untreated control group. The above mentioned results suggest that the treatment with prednisolone weaken the resistance of mice against N. fowleri infection, and probably induce more severe primary amoebic meningoencephalitis. Especially severe pathological findings were shown in pre-treated group, compared with untreated group.

복통환자에 있어 진경제(tiropramide hydrochloride)의 진통효과에 대한 연구

김옥용,박태진,김병성 대한가정의학회 1994 Korean Journal of Family Medicine Vol.15 No.8

Prednisolone 투여가 원발성 아메바성 뇌수막염의 실험적 발생에 미치는 영향(抄)

김옥용,임경일,이근태,최임순 임상약학사 1992 臨床藥學 Vol.12 No.7

흰쥐 신피질 세포막과 소포체 막의 calcium 수송에 미치는 cadmium 및 metallothionein의 영향에 관한 연구

최임순,김옥용,박용빈,Choe, Im-Sun,Kim, Ok-Yong,Park, Yong-Bin 한국통합생물학회 1993 동물학회지 Vol.36 No.4

흰쥐의 신피질 세포막과 소포체 막을 분리하여 카드를 및 Metallothioneln(MT)을 투여하였을 때 세포막에 존재하는 Ca-ATPase에 미치는 영향을 측정하여 다음과 같은 결과를 얻었다. 전기영동상에서 분리된 MT가 분자량 12KD 정도의 위치에 band가 나타났으며, 분리한 각 세포의 막에 카드윰을 농도별로 처리하였을 때 고농도일수록 Ca-ATPase의 활성도가 감소하였으나, MT를 처리한 경우 신피질 세포막은 거의 대조군과 유사한 결과를 나타냈고, 소포체 막에 MT를 처리한 경우는 20mg/ml의 카드윰을 처리한 경우와 유사하였다. 이와같은 결과로 보아 카드윰은 세포막의 Ca-ATPase의 활성을 저하시켜 세포내 칼슘 항상성에 영향을 미치는 MT는 Ca-ATPase의 활성을 회복시켜 카드뮴에 의한 세포독성의 방어작용에 부분적으로 작용한 것으로 생각된다.

Trichloroethylene , Tetrachloroethylene 및 1,1,1-Trichloroethane 이 흰쥐에 미치는 영향에 관한 조직학적 관찰

노명희,김옥용 ( Myung Hee Rho,Ok Yong Kim ) 한국환경생물학회 1990 환경생물 : 환경생물학회지 Vol.8 No.1

Effects of trichloroethylene (TrCE), tetrachloroethylene (TtCE) and 1, 1, 1-trichloroethane (1-TrCE) on the ultrastructural changes occuring in the liver, lung and spleen of rats were observed. Mitochondria were swollen and had dense aggregated matrix. Rough endoplasmic reticulums were vesiculated and free ribosomes were increased in cytoplasms of hepatocyte and splenic reticulocyte after treatment of these chemicals. These ultrastructural changes in TrCE treated group were more severe than those of TtCE or 1-TrCE treated group. When TtCE was treated, pathologic changes of lung cells were observed conspicuously comparing to the other cells, especially lysosomes were increased.

카드뮴에 의하여 유도되는 Metallothionein 의 흰쥐 간세포내 분포에 관한 연구

최임순,김옥용 ( Rim Soon Choe,Ok Yong Kim ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.1

This study was aimed to see the intracellular localization of Metallothionein which is induced in the hepatocytes of rats against the toxicity of cadmium. The Cd-MT made in the rat hepatocytes by injecting CdCl_2 was then given to a rabbit which produced metallothionein antibody. The avidin-biotin peroxidase complex method was applied to check the existance and induction of metallothionein in the hepatocytes. The serum of the non-immune rabbit did not show any response, while the control group rats showed uniform and weak response through the cytoplasm. But hardly any change was detected around the nucleus and part of the cells which showed a strong response close to their membranes. In the case of the cadmium treated cells, both the cytoplasm and nucleus showed a high sensitivity to the MT. Cells in which blebs were formed particularly sensitive in these blebs. It suggests that in the native state a small quantity of MT exists in the cytoplasm in the form of the compound with Zn. When the cytoplasm is exposed to Cd, MT production is induced. The reason for bleb formation can be Cd triggers impaired Ca^++ transport which increase the cytosolic Ca^++ level. The strong sensitivity of the blebs explain that MT is closely related with blebs, self-defense mechanism against the Cd toxicity.

열충격 및 ${\alpha}$-amanitin과 cycloheximide의 처리를 통한 초기 계배의 체절 형성 기작에 대한 발생학적 연구

최임순,박용빈,김옥용,Choe, Rim-Soon,Park, Yong-Bin,Kim, Ok-Yong 한국현미경학회 1991 Applied microscopy Vol.21 No.2

In order to investigate the factors of the control mechanism of somitogenesis, early chick embryos (H-H stage $8{\sim}13$) were treated with heat shock, ${\alpha}$-amanitin and cycloheximide and morphological changes of somite were examined by light microscopy, transmission and scanning electron microscopy. In normal chick embryo, somites were formed from the somitomere which preexisted in segmental plate. Somites were wrapped with extracellular collagen fibrils and connected with neural tube, notochord and ectoderm. And then, somites were differentiated to sclerotome, dermatome and myotome by the interaction of nervous tissue. Abnormal somites were observed after formation of six or seven so mites in heat shock treated group. Amounts of collagen fibrils were obviously decreased in this group. In cycloheximide treated group, most so mites were smaller and neural tube formation was incomplete. Chromatins were condenced and formed several heterochromatins in the nucleus of somite cells. Lipid like cytoplasmic dense mass and lipid droplets were also observed. Segmentation of somites seemed to be normal progress in ${\alpha}$-amanitin treated group. Center of somite, however, hollowed in longitudinal sectioned samples. These results suggested that so mites were already existed in the segmental plate as the form of somitomere. Segmented somites were contacted with neural tube or notochord and the somites were tightly connected with each other by the extracellular collagen fibrils which were secreted from neuroepithelium and somite cells. Somites are thought to differentiate into sclerotome, dermatome and myotome by these interactions.

일부 도시지역 주민의 가정의학과 인지도 및 가족기능도 지수

송애경,하장헌,김옥용,정수남,김병성 대한가정의학회 1992 Korean Journal of Family Medicine Vol.13 No.7

흰쥐 간조직에서의 비소처리 영향 및 비소 전처리 효과

노미경,손성향,부문종,김옥용,Ro, Mi-Kyeong,Sohn, Seong-Hyang,Boo, Moon-Jong,Kim, Ok-Yong 한국현미경학회 1994 Applied microscopy Vol.24 No.4

Sodium arsenite ($NaAsO_2$) was injected to the rat subcutaneously for the study of the acute toxicity of arsenite on hepatocytes, and the effects of pretreatment of arsenite and glutathione on the lethalty of the arsenite treated rats. Arsenite treated rat hepatocytes showed vacuolated cytosol and shrinked nuclear and expanded perinuclear space and cytoplasmic membrane whirl. Rats pretreated with BSO (L-Buthionine-SR-Sulfoximine), less survived than arsenite treated alone. It means that glutathione acts as a protecting agent against the arsenite. Subcutaneous sublethal dose (10mg/kg body weight) treatment was showed the protecting activity to lethality of lethal dose (15mg/kg body weight) treated rat. 10mg/kg body weight sublethal dose effects appeared in six hours intervals of between treatments.

위내시경 유소견자와 가족의 결속력·적응력과의 관계

하장헌,송애경,정수남,김옥용,김병성 대한가정의학회 1992 Korean Journal of Family Medicine Vol.13 No.10