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미세유체 디스크 칩에서 원심력을 이용한 극소 농도의 박테리아에 대한 대량의 초고속 항생제 감수성 검사 시스템
황순재(Sunjae Hwang),김승경(Seungkyoung Kim),최정일(Jungil Choi) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
Traditional AST technologies are time-consuming, so rapid AST technologies are being developed to compensate for these shortcomings. In particular, image-based rapid AST technologies are able to provide results more rapidly than conventional methods. However, these techniques require a high concentration of bacterial samples and take amount of time to obtain these samples. The method we have developed uses centrifugal force to concentrate bacterial samples at a low concentration in small volume chambers. The testing chamber has a micro-size well structure that prevents bacteria from escaping the observation area. Then, microscopic time-lapse observed the response of bacteria to antibiotics. For Escherichia. coli ATCC 25922 bacterial samples, about 16% concentration effect was confirmed at a concentration of 105 CFU/ml, and AST results were obtained in 2 hours.